|
| Status |
Public on Oct 03, 2012 |
| Title |
Clone A, 4T1 cells transduced with lentivirus encoding shRNAs to mouse Vav2 and Vav3 transcripts, biological rep1, Exp 1 |
| Sample type |
RNA |
| |
|
| Source name |
breast cancer cell line
|
| Organism |
Mus musculus |
| Characteristics |
cell line: 4T1
transducer: lentivirus encoding shRNAs to mouse Vav2 and Vav3 transcripts
|
| Treatment protocol |
Exponentially growing conditions
|
| Growth protocol |
All cells were incubated at 37ºC with 5% CO2. 4T1 cells were cultured in Roswell Park Memorial Institute (RPMI) media supplemented with 10% fetal calf serum (FCS), penicillin (10 μg/ml), and streptomycin (100 μg/ml).
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total cellular RNA was extracted from three independent exponential cultures of the appropriate cell lines using the RNAeasy kit (Qiagen)
|
| Label |
biotin
|
| Label protocol |
Total RNA was quantified using 6000 Nano Chips (Agilent Technologies, Santa Clara, CA) and used (2.3 μg/sample) to generated labeled cRNA probes according to the manufacturer’s instructions (Affymetrix).
|
| |
|
| Hybridization protocol |
Mouse Gene 1.0 ST arrays (Affymetrix) under standard conditions
|
| Scan protocol |
Affymetrix system
|
| Data processing |
Upon microarray hybridization, the raw data was normalized, filtered and analyzed with the Bioconductor software (www.bioconductor.com) using the Affy and Siggenes applications.
|
| |
|
| Submission date |
Oct 31, 2011 |
| Last update date |
Oct 03, 2012 |
| Contact name |
Ramon Garcia-Escudero |
| E-mail(s) |
ramon.garcia@ciemat.es, rgescudero@gmail.com
|
| Organization name |
CIEMAT
|
| Department |
Biomedical Innovation
|
| Lab |
Molecular and Traslational Oncology
|
| Street address |
Avda Complutense 40, Ed 70A
|
| City |
Madrid |
| State/province |
Madrid |
| ZIP/Postal code |
28040 |
| Country |
Spain |
| |
|
| Platform ID |
GPL6246 |
| Series (1) |
| GSE33348 |
The Rho Exchange Factors Vav2 and Vav3 Control a Lung Metastasis–Specific Transcriptional Program in Breast Cancer Cells |
|
