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Status |
Public on Oct 03, 2012 |
Title |
Clone A, 4T1 cells transduced with lentivirus encoding shRNAs to mouse Vav2 and Vav3 transcripts, biological rep4, Exp 2 |
Sample type |
RNA |
|
|
Source name |
breast cancer cell line
|
Organism |
Mus musculus |
Characteristics |
cell line: 4T1 transducer: lentivirus encoding shRNAs to mouse Vav2 and Vav3 transcripts
|
Treatment protocol |
Exponentially growing conditions
|
Growth protocol |
All cells were incubated at 37ºC with 5% CO2. 4T1 cells were cultured in Roswell Park Memorial Institute (RPMI) media supplemented with 10% fetal calf serum (FCS), penicillin (10 μg/ml), and streptomycin (100 μg/ml).
|
Extracted molecule |
total RNA |
Extraction protocol |
Total cellular RNA was extracted from three independent exponential cultures of the appropriate cell lines using the RNAeasy kit (Qiagen)
|
Label |
biotin
|
Label protocol |
Total RNA was quantified using 6000 Nano Chips (Agilent Technologies, Santa Clara, CA) and used (2.3 μg/sample) to generated labeled cRNA probes according to the manufacturer’s instructions (Affymetrix).
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|
|
Hybridization protocol |
Mouse Gene 1.0 ST arrays (Affymetrix) under standard conditions
|
Scan protocol |
Affymetrix system
|
Data processing |
Upon microarray hybridization, the raw data was normalized, filtered and analyzed with the Bioconductor software (www.bioconductor.com) using the Affy and Siggenes applications.
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|
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Submission date |
Oct 31, 2011 |
Last update date |
Oct 03, 2012 |
Contact name |
Ramon Garcia-Escudero |
E-mail(s) |
ramon.garcia@ciemat.es, rgescudero@gmail.com
|
Organization name |
CIEMAT
|
Department |
Biomedical Innovation
|
Lab |
Molecular and Traslational Oncology
|
Street address |
Avda Complutense 40, Ed 70A
|
City |
Madrid |
State/province |
Madrid |
ZIP/Postal code |
28040 |
Country |
Spain |
|
|
Platform ID |
GPL6246 |
Series (1) |
GSE33348 |
The Rho Exchange Factors Vav2 and Vav3 Control a Lung Metastasis–Specific Transcriptional Program in Breast Cancer Cells |
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