|
| Status |
Public on Nov 08, 2011 |
| Title |
wild-type, passage 6 MEFs from mixed BL background 1 |
| Sample type |
RNA |
| |
|
| Source name |
wild-type, passage 6 MEFs from mixed BL background
|
| Organism |
Mus musculus |
| Characteristics |
passage: early
genetic background: mixed 129SVJ/NIH Black Swiss (BL)
genotype: wild-type
cell type: embryonic fibroblast
|
| Treatment protocol |
not applicable
|
| Growth protocol |
MEFs derived from day 11.5 mouse embryos, culturing in Dulbecco’s MEM supplemented with 10% fetal bovine serum and 100 units/ml penicillin-streptomycin.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was isolated from MEFs with the RNeasy Mini Kit (Qiagen), according to the manufacturer’s instructions.
|
| Label |
biotin
|
| Label protocol |
Samples were enzymatically fragmented and biotinylated using the WT Terminal Labeling Kit (Affymetrix)
|
| |
|
| Hybridization protocol |
Samples were hybridized with GeneChip Mouse Exon 1.0 ST Arrays (Affymetrix) and scanned at the NCI-Frederick Microarray Core Facility.
|
| Scan protocol |
Array scanning was performed according to the manufacturer's instruction (Affymetrix)
|
| Description |
MEFs from a mix of 2 E14.5 embryos
|
| Data processing |
Data were processed using GeneSpringGX 11.5 software with guided workflow. Gene level expression were summarized after quantile normalized.
MoEx-1_0-st-v1.r2.pgf
MoEx-1_0-st-v1.r2.dt1.mm8.mps
Notes : Created from guided workflow step : Filter on Probesets
Entity List :All Entities
Interpretation :All Samples
Experiment :Smurf2MEF
Percentile cut-off :20.0
Entities retained in which atleast 1 out of 8 sample values above cut-off
Technology : Affymetrix.ExonExprChip.MoGene-1_0-st-v1_na30_1_mm9_2009-12-08
|
| |
|
| Submission date |
Nov 06, 2011 |
| Last update date |
Nov 08, 2011 |
| Contact name |
Ying E Zhang |
| E-mail(s) |
zhangyin@mail.nih.gov
|
| Organization name |
National Cancer Institute, NIH
|
| Department |
LCMB/CCR
|
| Lab |
Ying E Zhang
|
| Street address |
37 Convent Dr., Bldg.37, Rm.2056
|
| City |
Bethesda |
| State/province |
Maryland |
| ZIP/Postal code |
20892 |
| Country |
USA |
| |
|
| Platform ID |
GPL6246 |
| Series (1) |
| GSE33502 |
Gene Level Expression Profiling of MEFs derived from wild type and Smurf2-/- embryos |
|
