|
| Status |
Public on Jun 10, 2024 |
| Title |
Q146A-A |
| Sample type |
SRA |
| |
|
| Source name |
hyperphosphorylated mutant
|
| Organism |
Streptococcus pyogenes |
| Characteristics |
strain: hyperphosphorylated mutant
replicate: A
growth condition: in vitro
chip antibody: custom, rabbit anti-LiaR
|
| Treatment protocol |
No treatment
|
| Growth protocol |
GAS cells were grown in rich medium (Todd-Hewitt, 0.2% yeast extract) to an OD of 0.5 (mid-exponential phase)
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
Proteins crosslinked to DNA with 1% formaldehyde, cells harvested by centrifugation, extraction using sonication as described in methods
Libraries constructed by Genewiz (Azenta Life Sciences)
|
| |
|
| Library strategy |
ChIP-Seq |
| Library source |
genomic |
| Library selection |
ChIP |
| Instrument model |
Illumina NovaSeq 6000 |
| |
|
| Description |
Q146A_peaks_05-30-2024.xlsx
|
| Data processing |
Base calls onboard Illumina HiSeq
Raw data files processed using CLC Genomics Workbench v23
Reads mapped to reference genome MGAS10870 (CP067090.1)
Peaks called using Transcription Factor ChIP-seq module (CLC Genomics Workbench v23)
Assembly: MGAS10870 (CP067090.1)
Supplementary files format and content: Spreadsheet, peak score
|
| |
|
| Submission date |
Jun 02, 2024 |
| Last update date |
Jun 10, 2024 |
| Contact name |
Anthony Flores |
| E-mail(s) |
anthony.r.flores@uth.tmc.edu
|
| Phone |
7135006626
|
| Organization name |
UTHSC/McGovern Medical School
|
| Street address |
6431 Fannin St., MSB 3.130
|
| City |
Houston |
| State/province |
TX |
| ZIP/Postal code |
77030 |
| Country |
USA |
| |
|
| Platform ID |
GPL28678 |
| Series (1) |
| GSE268862 |
LiaR-dependent gene expression contributes to antimicrobial responses in group A Streptococcus [ChIP-seq] |
|
| Relations |
| BioSample |
SAMN41642012 |
| SRA |
SRX24776303 |
