|
| Status |
Public on Nov 16, 2011 |
| Title |
Podocyte cells RPMI Replicate 1 |
| Sample type |
RNA |
| |
|
| Source name |
Podocyte cells RPMI
|
| Organism |
Mus musculus |
| Characteristics |
cell type: Primary podocyte cell
media: RPMI
genetic background: mixed 75% Sv129
|
| Treatment protocol |
Changing the medium 1-2 times weekly
|
| Growth protocol |
Cultivated capsulated or decapsulated glomeruli for 7-14 days in EGM-MV Medium
|
| Extracted molecule |
total RNA |
| Extraction protocol |
trypsinisation the cells, resuspending the cells in RPMI
|
| Label |
biotin
|
| Label protocol |
Treating the suspended cells for 1 minute at 37°C with 2mM fluorescein di-ß-Galactopyranoside
|
| |
|
| Hybridization protocol |
Samples were diluted 10-fold with RPMI-1640, incubate for 30-60 minutes on ice
|
| Scan protocol |
Affymetrix standard scan conditions
|
| Description |
FACs sorted cells from transgenic mice
|
| Data processing |
JMP Genomics 4.0, RMA background correction, quantile normalization
|
| |
|
| Submission date |
Nov 15, 2011 |
| Last update date |
Nov 29, 2011 |
| Contact name |
Gregor Warsow |
| Organization name |
University of Rostock
|
| Department |
Medical Department
|
| Lab |
IBIMA
|
| Street address |
Ernst-Heydemann-Str. 8
|
| City |
Rostock |
| ZIP/Postal code |
18057 |
| Country |
Germany |
| |
|
| Platform ID |
GPL14866 |
| Series (1) |
| GSE33714 |
Primary cultures of glomerular parietal epithelial cells or podocytes with proven origin |
|
