|
| Status |
Public on Nov 01, 2012 |
| Title |
breast tumor part_IDC_rep1_4411 |
| Sample type |
RNA |
| |
|
| Channel 1 |
| Source name |
Infiltrating Ductal Carcinoma (IDC), Buffer 1, ER(-), PR(-), HER2(-).
|
| Organism |
Homo sapiens |
| Characteristics |
tissue type: breast tumor
cell type: Infiltrating Ductal Carcinoma (IDC)
array id: 4411
er ihc: 0
pr ihc: 0
her-2/neu ihc: 0
age: 58
ajcc stage: IIB
lymph node metastasis: 1
lymphovascular invasion: 1
grade: 3
mitotic count: 3
nuclear pleomorphism: 3
tubule formation: 2
tumor size: 2
distant metastasis-free (years): 2.11
event of distant metastasis: 0
event of death: 0
|
| Treatment protocol |
The breast tumor samples were excised, collected, and snap-frozen in liquid nitrogen immediately before RNA extraction.
|
| Growth protocol |
The breast tumor samples were immediately frozen by liquid nitrogen without any additional growth procedure before RNA extraction.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol reagent combined with RNAeasy mini kit
|
| Label |
Cy5
|
| Label protocol |
Agilent Low RNA Input Fluorescent Linear Amplification Kit, protocol version 2.0
|
| |
|
| Channel 2 |
| Source name |
Stratagene's human common reference RNA
|
| Organism |
Homo sapiens |
| Characteristics |
sample type: Commercially-available reference
|
| Treatment protocol |
The breast tumor samples were excised, collected, and snap-frozen in liquid nitrogen immediately before RNA extraction.
|
| Growth protocol |
The breast tumor samples were immediately frozen by liquid nitrogen without any additional growth procedure before RNA extraction.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol reagent combined with RNAeasy mini kit
|
| Label |
Cy3
|
| Label protocol |
Agilent Low RNA Input Fluorescent Linear Amplification Kit, protocol version 2.0
|
| |
|
| |
| Hybridization protocol |
Agilent Oligo Microarray Kit, hybridization protocol version 5.0
|
| Scan protocol |
Agilent scanner and Feature Extraction 6.0.
|
| Description |
NTUH_TN_35
|
| Data processing |
The log2-ratio gene expression data in the microarray dataset were normalized by quantile normalization.
|
| |
|
| Submission date |
Nov 23, 2011 |
| Last update date |
Jan 02, 2014 |
| Contact name |
King-Jen Chang |
| Organization name |
National Taiwan University Hospital
|
| Department |
Department of Surgery
|
| Street address |
No.1, Changde St., Zhongzheng Dist.
|
| City |
Taipei |
| ZIP/Postal code |
100 |
| Country |
Taiwan |
| |
|
| Platform ID |
GPL7264 |
| Series (2) |
| GSE33926 |
Molecular characteristics and metastasis predictor genes of triple-negative breast cancer |
| GSE53752 |
Molecular characteristics and metastasis predictor genes of triple-negative breast cancer (II) |
|
