|
| Status |
Public on Oct 22, 2013 |
| Title |
pCR+npCR mamma breast cancer BC_5342587014_E |
| Sample type |
RNA |
| |
|
| Source name |
14G core needle breast tumor biopsy
|
| Organism |
Homo sapiens |
| Characteristics |
response breast: pCR+npCR mamma [pathological complete response + near pathological complete response mamma]
response: CR+kl [(pathological) complete response breast, positive lymph nodes]
ihc er percentage: 0
ihc pr percentage: 0
intrinsic molecular subtype: Her2
treatment: PTC
clinical subtype: HER2
tissue: breast cancer tumor
|
| Extracted molecule |
total RNA |
| Extraction protocol |
From the frozen biopsies approximately 30 sections of 30-μm thickness were used for total RNA isolation with RNABee (Campro Scientific, Amersfoort, The Netherlands). Isolated total RNA was subsequently DNase-treated by using the Qiagen RNase-free DNase kit and RNeasy spin columns (Qiagen, West Sussex, U.K.) and dissolved in RNase-free H2O.
|
| Label |
Biotin
|
| Label protocol |
Total RNA was amplified and biotin labeled using the Illumina® TotalPrep™-96 RNA kit. All steps were performed according to standard protocol.
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol
|
| Scan protocol |
Standard Illumina scanning protocol
|
| Description |
BC_5342587014_E
|
| Data processing |
The data were between-array normalised using simple scaling (SSN) subsequently log2 transformed
|
| |
|
| Submission date |
Dec 05, 2011 |
| Last update date |
Oct 22, 2013 |
| Contact name |
Jelle Johannes ten Hoeve |
| E-mail(s) |
j.t.hoeve@nki.nl
|
| Organization name |
Netherlands Cancer Institute
|
| Department |
Molecular Biology
|
| Lab |
Bioinformatics and Statistics
|
| Street address |
Plesmanlaan 121
|
| City |
Amsterdam |
| ZIP/Postal code |
1066 CX |
| Country |
Netherlands |
| |
|
| Platform ID |
GPL6884 |
| Series (1) |
| GSE41656 |
Gene expression analysis of a series of HER2+ of breast tumors |
|
