|
| Status |
Public on Dec 13, 2011 |
| Title |
MNT3 |
| Sample type |
RNA |
| |
|
| Source name |
non-treated REC, 24 hr
|
| Organism |
Homo sapiens |
| Characteristics |
cell type: retinal endothelial cells
treatment: none
|
| Treatment protocol |
4 dishes remained untreated. 4 dishes treated with 4ug/ml melphalan for 24 hours. All cells collected into Trizol.
|
| Growth protocol |
Human retinal endothelial cells were grown in M131 medium until 90% confluence.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Extraction of total RNA was performed using RNA Stat-60, according to the manufacturer's instructions. RNA purity was checked by OD260/280 readings, and RNA integrity was checked using Agilent Bioanalyzer capillary electrophoresis profiles.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cDNA were prepared from 200 ng total RNA according to the standard whole transcript labeling protocol for Affymetrix ST arrays (Ambion WT Expression Kit Manual, 2010, Ambion; GeneChip Whole Transcript Sense Target Labeling Kit Manual, 2007, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 15 ug of cDNA were hybridized for 16 hr at 45C on GeneChip Human Gene 1.0 ST arrays. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
| Scan protocol |
GeneChips were scanned using the Affymetrix GCS 3000 7G.
|
| Description |
cell culture extract
|
| Data processing |
The data were analyzed in Expression Console using Affymetrix default analysis settings for RMA quantile normalization.
|
| |
|
| Submission date |
Dec 12, 2011 |
| Last update date |
Sep 01, 2016 |
| Contact name |
Jena J Steinle |
| E-mail(s) |
jsteinl1@uthsc.edu
|
| Organization name |
University of Tennessee Health Science Center
|
| Department |
Ophthalmology
|
| Lab |
Steinle Lab
|
| Street address |
930 Madison Ave, Suite 768
|
| City |
Memphis |
| State/province |
TN |
| ZIP/Postal code |
38163 |
| Country |
USA |
| |
|
| Platform ID |
GPL6244 |
| Series (2) |
| GSE34381 |
Melphalan-treated human retinal endothelial cells |
| GSE34389 |
Cell leukostasis during organ-targeted chemotherapy in human retinal endothelial cells and rhesus macaques endothelial cells |
|
| Relations |
| Reanalyzed by |
GSE86357 |
