|
| Status |
Public on Aug 15, 2012 |
| Title |
Down syndrome with Atrial Septal Defect 02-247 |
| Sample type |
RNA |
| |
|
| Source name |
lymphoblastoid cell line
|
| Organism |
Homo sapiens |
| Characteristics |
gender: Male
age (y): 5
karyotype: Trisomic 21
cell type: lymphoblastoid cell line
phenotype: Down syndrome Down syndrome with Atrial Septal Defect
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted with Macherey-Nagel RNAII mini-kit in accordance with the prescribed protocol provided with the kit. Quality control was performed with Agilent Bioanalyser.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared with the Ambion MessageAmp kit for Illumina arrays
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol
|
| Scan protocol |
Standard Illumina scanning protocol
|
| Description |
replicate 3
|
| Data processing |
The data were normalised using quantile normalisation with IlluminaGUI in R
|
| |
|
| Submission date |
Dec 14, 2011 |
| Last update date |
Aug 15, 2012 |
| Contact name |
Luce Dauphinot |
| E-mail(s) |
luce.dauphinot@upmc.fr
|
| Phone |
33 1 57274518
|
| Organization name |
INSERM U1127-CNRS UMR7225-UPMC
|
| Department |
ICM
|
| Lab |
Alzheimer and Prions Disease team
|
| Street address |
47 boulevard de l'hôpital
|
| City |
Paris |
| ZIP/Postal code |
75013 |
| Country |
France |
| |
|
| Platform ID |
GPL6102 |
| Series (2) |
| GSE34457 |
Molecular Signatures of cardiac defects in Down syndrome lymphoblastoid cell lines (congenital heart disease) |
| GSE34459 |
Molecular Signatures of cardiac defects in Down syndrome lymphoblastoid cell lines |
|
