|
| Status |
Public on Dec 30, 2011 |
| Title |
Cardiomyocytes-Ad-β-catenin-GFP, biological rep2 |
| Sample type |
RNA |
| |
|
| Source name |
Neonatal rat cardiomyocytes infected with Ad-β-catenin-GFP
|
| Organism |
Rattus norvegicus |
| Characteristics |
tissue: ventricular myocardium
strain: Sprague-Dawley
age: 1- to 2-day old
infection: Ad-β-catenin-GFP
|
| Treatment protocol |
Cardiomyocytes were infected with GFP control or β-catenin adenovirus and cultured with serum-free DMEM/F12 for 24 hours.
|
| Growth protocol |
Primary cardiomyocytes were prepared by enzymatic disassociation of 1- to 2-day old Sprague-Dawley rats, the isolated cardiomyocytes resuspended in fresh DMEM/F12 (HyClone) containing 10% FBS and 1% penicillin-streptomycin were plated into the Laminin (Sigma) precoated dishes (Corning) and incubated for 24 h at 37 ℃.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Extraction of total RNA was performed using TRIzol according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5ug total RNA (Expression Analysis Technical Manual, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45°C on GeneChip rat 230 2.0 Genome Array in the Affymetrix Hybridization Oven 640. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
| Scan protocol |
GeneChips were scanned using the affymetrix scanner 3000 7G.The scanned images were first assessed by visual inspection then analyzed to generate raw data files saved as CEL files using the default setting of AGCC.
|
| Description |
Gene expression data from neonatal rat cardiomyocytes infected with Ad-β-catenin-GFP
|
| Data processing |
The data were analyzed with GeneSpring GX 11.0 Software using RMA algorithm.
|
| |
|
| Submission date |
Dec 29, 2011 |
| Last update date |
Dec 30, 2011 |
| Contact name |
junjie li |
| Organization name |
PUMC
|
| Street address |
#5 Dong Dan San Tiao
|
| City |
Beijing |
| ZIP/Postal code |
100005 |
| Country |
China |
| |
|
| Platform ID |
GPL1355 |
| Series (1) |
| GSE34772 |
Expression data from Ad-β-catenin-GFP or Ad-GFP infected cardiomyocytes |
|
