|
| Status |
Public on Jan 10, 2012 |
| Title |
B6g7 DN2-3 replicate 1 |
| Sample type |
RNA |
| |
|
| Source name |
B6g7 DN2-3
|
| Organism |
Mus musculus |
| Characteristics |
strain background: B6g7
genotype/variation: non-Tg
gender: female
age: 4 weeks
isolation/cell type: CD4 and CD8 complement-mediated depletion followed by FACS sorting of CD4-CD8-Lin-CD25+ thymocytes (DN2-3)
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA by Trizol method
|
| Label |
biotin
|
| Label protocol |
standard Affymetrix protocol
|
| |
|
| Hybridization protocol |
standard Affymetrix protocol
|
| Scan protocol |
standard Affymetrix protocol
|
| Description |
DN2-3.B6g7#1
|
| Data processing |
The datasets was pre-filtered to keep only those probesets for which a gene symbol could be found in the Affymetrix annotation. CEL files were normalized using Affymetrix Power Tools on the predefined probeset id list mentioned above, and using the standard RMA workflow (background adjustment, quantile normalization, median polish probeset summarization).
|
| |
|
| Submission date |
Jan 09, 2012 |
| Last update date |
Jan 10, 2012 |
| Contact name |
CBDM Lab |
| E-mail(s) |
cbdm@hms.harvard.edu
|
| Phone |
617-432-7747
|
| Organization name |
Harvard Medical School
|
| Department |
Microbiology and Immunobiology
|
| Lab |
CBDM
|
| Street address |
77 Avenue Louis Pasteur
|
| City |
Boston |
| State/province |
MA |
| ZIP/Postal code |
02215 |
| Country |
USA |
| |
|
| Platform ID |
GPL6246 |
| Series (2) |
| GSE34935 |
Expression data from BDC2.5 TCR Tg thymocytes on B6g7 and NOD background |
| GSE34936 |
NOD genetic variation influences ab/gd lineage decisions when TCRa is prematurely expressed, but not the process of negative selection. |
|
