NCBI Logo
GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
          Go
Sample GSM86437 Query DataSets for GSM86437
Status Public on Dec 09, 2005
Title mMSC CD34+ 1, chip MG_U74Cv2
Sample type RNA
 
Source name Bone marrow stromal cell clone, Bone marrow
Organism Mus musculus
Characteristics Strain: C57Bl/6
Gender: Female
Defining markers: CD34+, CD117-, Tie-2-,FLK-1-, FLT-1-, CD44-
Growth protocol Growth medium: DMEM
Media supplements: 10% fetal bovine serum
Culture conditions: 37C, 5% CO2
Extracted molecule total RNA
Extraction protocol RNA prep method: RNeasy
Cell purification method: Single cell colony isolation
Contaminating cell type: Other bone marrow cells
Method for estimating purity: FACS analysis with CD34+ marker
Label Biotin
 
Description This sample was analyzed as part of the Stem Cell Genomics Project (http://www.scgp.ca:8080/StemBase/). The biological material was submitted to the Ontario Genomics Innovation Centre (http://www.ottawagenomecenter.ca/) by Dr. Jacques Galipeau (jgalipea@lab.jgh.mcgill.ca; Sir Mortimer B. Davis-Jewish General Hospital; 3755 Cote Ste-Catherine Road) for analysis.
Stembase Experiment ID: E3
Stembase Experiment ID link: http://www.scgp.ca:8080/StemBase/?path=/browse/experiment&id=3
SCGP Sample ID: S4
SCGP Sample ID link: http://www.scgp.ca:8080/StemBase/?path=/browse/experiment&id=3#SAMPLE_3
Short description: CD34+ cells isolated from adult bone marrow. Subsequently, pure colonies were selected and characterized.
Analysis requested: Compare to control/normal marrow stromal cells previously analyzed for J. Galipeau - Murine genome U74A-C
Estimated purity: 100%
RNA concentration: 0.573 ug/ul
Num cells for RNA prep: 4x10e6
Sample volume: 26.1 ul
Data processing Calculated using the MAS5 algorithm where sc=1500, tau=0.015, alpha1=0.04, and alpla2=0.06
 
Submission date Dec 07, 2005
Last update date Jan 19, 2006
Organization Ottawa Hospital Research Institute
Phone (613) 737-8899 -73255
Department Cellular and Molecular Medicine
Lab Ottawa Bioinformatics Core Facility
Street address 501 Smyth Rd.
City Ottawa
State/province ON
ZIP/Postal code K1H 8L6
Country Canada
 
Platform ID GPL83
Series (1)
GSE3764 Expression Profile of Homogenous Bone Marrow Stromal Stem Cell Clones

Data table header descriptions
ID_REF
VALUE 'signal' a measure of the abundance of a transcript. Calculated using the MAS5 algorithm where sc=1500, tau=0.015, alpha1=0.04, and alpla2=0.06.
ABS_CALL the call in an absolute analysis that indicates if the transcript was present (P), absent (A), marginal (M), or no call (NC)
DETECTION P-VALUE 'detection p-value', p-value that indicates the significance level of the detection call

Data table
ID_REF VALUE ABS_CALL DETECTION P-VALUE
AFFX-MurIL2_at 754.619 A 0.672921
AFFX-MurIL10_at 2257.61 A 0.41138
AFFX-MurIL4_at 1044.42 A 0.0956669
AFFX-MurFAS_at 1011.06 A 0.41138
AFFX-BioB-5_at 9484.93 P 0.00255552
AFFX-BioB-M_at 21282.9 P 0.000445901
AFFX-BioB-3_at 14581 P 0.000662269
AFFX-BioC-5_at 26191.7 P 0.000296708
AFFX-BioC-3_at 22126.8 P 5.16732e-05
AFFX-BioDn-5_at 27532.3 P 0.000169227
AFFX-BioDn-3_at 154610 P 8.14279e-05
AFFX-CreX-5_at 314046 P 4.42873e-05
AFFX-CreX-3_at 480258 P 4.42873e-05
AFFX-BioB-5_st 1238.01 A 0.39692
AFFX-BioB-M_st 2247.22 A 0.51489
AFFX-BioB-3_st 687.623 A 0.814869
AFFX-BioC-5_st 433.917 A 0.883887
AFFX-BioC-3_st 139.052 A 0.804734
AFFX-BioDn-5_st 712.538 A 0.39692
AFFX-BioDn-3_st 4901.58 A 0.0726999

Total number of rows: 11934

Table truncated, full table size 345 Kbytes.




Supplementary file Size Download File type/resource
GSM86437.CEL.gz 2.6 Mb (ftp)(http) CEL
GSM86437.EXP.gz 523 b (ftp)(http) EXP

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap