|
| Status |
Public on Dec 01, 2012 |
| Title |
ARCaP-E 5-aza 2 |
| Sample type |
RNA |
| |
|
| Source name |
ARCaP-E prostate cancer cells, 5-aza-deoxycytidine
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: ARCaP-E
cell type: prostate cancer
treatment: 5-aza-deoxycytidine
|
| Treatment protocol |
RNA was isolated from three independent experiments in which ARCaP-E and ARCaP-M cells were treated with DMSO control, 20 µM genistein, or 1 µM 5-aza-deoxycytidine for 6 days, 1µM vorinostat for 48 hrs, or a combination of 1µM vorinostat (48 hrs) plus 20 µM genistein (6 days).
|
| Growth protocol |
ARCaP-E and ARCaP-M cells were purchased from Novicure Biotechnology and propagated in MCaP-Medium (Novicure Biotechnology, Inc, Birmingham, AL), supplemented with 5% FBS, 200mM L-glutamine, and pen-strep.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated using RNeasy kits (QIAGEN, Valencia, CA) according to manufacturer instructions.
|
| Label |
Biotin
|
| Label protocol |
PCR product was prepared with standard Illumina protocol for arrays.
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol.
|
| Scan protocol |
BeadChips were scanned using an Illumina BeadArray Scanner.
|
| Data processing |
The data were normalized using quantile-normalization with GenomeStudio V2010.2.
|
| |
|
| Submission date |
Jan 20, 2012 |
| Last update date |
Dec 01, 2012 |
| Contact name |
Khanjan Gandhi |
| E-mail(s) |
khanjan.gandhi@emory.edu
|
| Organization name |
Winship Cancer Institute, Emory University.
|
| Street address |
1365-B Clifton Rd NE, Room 4107
|
| City |
Atlanta |
| State/province |
Georgia |
| ZIP/Postal code |
30363 |
| Country |
USA |
| |
|
| Platform ID |
GPL10558 |
| Series (2) |
| GSE35242 |
Genistein Cooperates with the Histone Deacetylase Inhibitor Vorinostat to Induce Cell Death in Prostate Cancer Cells (expression data) |
| GSE35246 |
Genistein Cooperates with the Histone Deacetylase Inhibitor Vorinostat to Induce Cell Death in Prostate Cancer Cells |
|
