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Status |
Public on Dec 01, 2012 |
Title |
ARCaP-M DMSO 1R |
Sample type |
RNA |
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|
Source name |
ARCaP-M prostate cancer cells, DMSO
|
Organism |
Homo sapiens |
Characteristics |
cell line: ARCaP-M cell type: prostate cancer treatment: DMSO
|
Treatment protocol |
RNA was isolated from three independent experiments in which ARCaP-E and ARCaP-M cells were treated with DMSO control, 20 µM genistein, or 1 µM 5-aza-deoxycytidine for 6 days, 1µM vorinostat for 48 hrs, or a combination of 1µM vorinostat (48 hrs) plus 20 µM genistein (6 days).
|
Growth protocol |
ARCaP-E and ARCaP-M cells were purchased from Novicure Biotechnology and propagated in MCaP-Medium (Novicure Biotechnology, Inc, Birmingham, AL), supplemented with 5% FBS, 200mM L-glutamine, and pen-strep.
|
Extracted molecule |
total RNA |
Extraction protocol |
Total RNA was isolated using RNeasy kits (QIAGEN, Valencia, CA) according to manufacturer instructions.
|
Label |
Biotin
|
Label protocol |
PCR product was prepared with standard Illumina protocol for arrays.
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|
|
Hybridization protocol |
Standard Illumina hybridization protocol.
|
Scan protocol |
BeadChips were scanned using an Illumina BeadArray Scanner.
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Data processing |
The data were normalized using quantile-normalization with GenomeStudio V2010.2.
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Submission date |
Jan 20, 2012 |
Last update date |
Dec 01, 2012 |
Contact name |
Khanjan Gandhi |
E-mail(s) |
khanjan.gandhi@emory.edu
|
Organization name |
Winship Cancer Institute, Emory University.
|
Street address |
1365-B Clifton Rd NE, Room 4107
|
City |
Atlanta |
State/province |
Georgia |
ZIP/Postal code |
30363 |
Country |
USA |
|
|
Platform ID |
GPL10558 |
Series (2) |
GSE35242 |
Genistein Cooperates with the Histone Deacetylase Inhibitor Vorinostat to Induce Cell Death in Prostate Cancer Cells (expression data) |
GSE35246 |
Genistein Cooperates with the Histone Deacetylase Inhibitor Vorinostat to Induce Cell Death in Prostate Cancer Cells |
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