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GEO help: Mouse over screen elements for information. |
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| Status |
Public on Dec 09, 2005 |
| Title |
C2C12 (2), chip MG_U74Cv2 |
| Sample type |
RNA |
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| Source name |
C2C12, Skeletal muscle
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| Organism |
Mus musculus |
| Characteristics |
Strain: C3H
Defining markers: Myosin heavy chain, myogenin
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| Biomaterial provider |
Contact details for sample vendor: ATCC
Vendor catalogue number: CRL-1772
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| Growth protocol |
Growth medium: DMEM
Media supplements: 2% horse serum, 1X penicillin/streptomycin
Culture conditions: 37C
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| Extracted molecule |
total RNA |
| Extraction protocol |
RNA prep method: RNeasy
Cell purification method: Established cell line
Method for estimating purity: O.D., formaldehyde gel
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| Label |
Biotin
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| Description |
This sample was analyzed as part of the Stem Cell Genomics Project (http://www.scgp.ca:8080/StemBase/). The biological material was submitted to the Ontario Genomics Innovation Centre (http://www.ottawagenomecenter.ca/) by Dr. Lynn Megeney (lmegeney@ohri.ca; 501 Smyth Road, Room 4E102) for analysis.
Stembase Experiment ID: E1
Stembase Experiment ID link: http://www.scgp.ca:8080/StemBase/?path=/browse/experiment&id=1
SCGP Sample ID: S2
SCGP Sample ID link: http://www.scgp.ca:8080/StemBase/?path=/browse/experiment&id=1#SAMPLE_1
Short description: RNA extracted from C2C12 mouse skeletal muscle cells following 5 days of culture in differentiation medium.
Estimated purity: 100%
RNA concentration: 2.0 ug/ul
Num cells for RNA prep: 2x10e7
Sample volume: 10.0 ul
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| Data processing |
Calculated using the MAS5 algorithm where sc=1500, tau=0.015, alpha1=0.04, and alpla2=0.06
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| Submission date |
Dec 08, 2005 |
| Last update date |
Jan 19, 2006 |
| Organization |
Ottawa Hospital Research Institute |
| Phone |
(613) 737-8899 -73255
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| Department |
Cellular and Molecular Medicine
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| Lab |
Ottawa Bioinformatics Core Facility
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| Street address |
501 Smyth Rd.
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| City |
Ottawa |
| State/province |
ON |
| ZIP/Postal code |
K1H 8L6 |
| Country |
Canada |
| |
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| Platform ID |
GPL83 |
| Series (1) |
| GSE3787 |
C2C12 Mouse Skeletal Muscle Cells Grown in Differentiation Medium for 5 Days. |
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| Data table header descriptions |
| ID_REF |
|
| VALUE |
'signal' a measure of the abundance of a transcript. Calculated using the MAS5 algorithm where sc=1500, tau=0.015, alpha1=0.04, and alpla2=0.06. |
| ABS_CALL |
the call in an absolute analysis that indicates if the transcript was present (P), absent (A), marginal (M), or no call (NC) |
| DETECTION P-VALUE |
'detection p-value', p-value that indicates the significance level of the detection call |
| Data table |
| ID_REF |
VALUE |
ABS_CALL |
DETECTION P-VALUE |
| AFFX-MurIL2_at |
308.141 |
A |
0.783476 |
| AFFX-MurIL10_at |
418.134 |
A |
0.544587 |
| AFFX-MurIL4_at |
610.683 |
A |
0.275146 |
| AFFX-MurFAS_at |
2072.85 |
P |
0.0125468 |
| AFFX-BioB-5_at |
5943.65 |
P |
0.0125468 |
| AFFX-BioB-M_at |
13318.7 |
P |
0.000662269 |
| AFFX-BioB-3_at |
8202.16 |
P |
0.00159257 |
| AFFX-BioC-5_at |
18708.4 |
P |
0.000581214 |
| AFFX-BioC-3_at |
13972 |
P |
5.16732e-05 |
| AFFX-BioDn-5_at |
22250.9 |
P |
0.000146581 |
| AFFX-BioDn-3_at |
114901 |
P |
0.000146581 |
| AFFX-CreX-5_at |
229292 |
P |
4.42873e-05 |
| AFFX-CreX-3_at |
301530 |
P |
4.42873e-05 |
| AFFX-BioB-5_st |
1670.45 |
A |
0.195266 |
| AFFX-BioB-M_st |
433.106 |
A |
0.659339 |
| AFFX-BioB-3_st |
522.899 |
A |
0.794268 |
| AFFX-BioC-5_st |
313.648 |
A |
0.843268 |
| AFFX-BioC-3_st |
572.95 |
A |
0.58862 |
| AFFX-BioDn-5_st |
2241.11 |
A |
0.250796 |
| AFFX-BioDn-3_st |
2789.69 |
A |
0.147939 |
Total number of rows: 11934
Table truncated, full table size 346 Kbytes.
| Supplementary file |
Size |
Download |
File type/resource |
| GSM86775.CEL.gz |
2.6 Mb |
(ftp)(http) |
CEL |
| GSM86775.EXP.gz |
520 b |
(ftp)(http) |
EXP |
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