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Sample GSM868304 Query DataSets for GSM868304
Status Public on Sep 29, 2012
Title Biogel M2
Sample type RNA
Source name biogel-elicited murine peritoneal macrophages, incubated in OptiMEM-10 + IL-4 (supernatant from IL-4 producing cell line) for 24 hrs on tissue culture plastic
Organism Mus musculus
Characteristics strain: C57BL/6J
replicate: 3
cell type: biogel-elicited murine peritoneal macrophages
treatment: incubated in OptiMEM-10 + IL-4 (supernatant from IL-4 producing cell line) for 24 hrs on tissue culture plastic
Treatment protocol Cells were treated for 24 hours with IL-4 (supernatant of mIL-4 cDNA transfected X63Ag8-653 plasmcytoma cell line).
Growth protocol Mouse thioglycollate and Biogel elicited macrophages were isolated by adhesion and cultured on Pemanox or Tissue culture plastic, in RPMI 1640 .
Extracted molecule total RNA
Extraction protocol RNA was extracted with QIAGEN RNeasy mini kit in accordance with the prescribed protocol provided with the kit. Quality control was performed with Agilent Bioanalyser.
Label biotin
Label protocol Affymetrix standard protocol
Hybridization protocol Affymetrix standard protocol
Scan protocol Affymetrix standard protocol
Description Replicate 3
Data processing RMA in Affy R bioconductor
Submission date Jan 30, 2012
Last update date Apr 12, 2018
Contact name Fernando O Martinez Estrada
Organization name University of Surrey
Department Faculty of Health & Medical Sciences
Lab Innate Immunity
Street address Stag Hill
City Guildford
State/province Surrey
ZIP/Postal code GU2 7XH
Country United Kingdom
Platform ID GPL1261
Series (2)
GSE35436 Genome-wide analysis of mouse macrophages stimulated with IL-4 (Biogel and thioglycollate macrophages) (Affymetrix)
GSE35495 Genome-wide analysis of human and mouse macrophages, resting and activated

Data table header descriptions

Data table
1415670_at 9.752227616
1415671_at 12.39930374
1415672_at 12.33160122
1415673_at 9.207086237
1415674_a_at 10.1967209
1415675_at 10.1373169
1415676_a_at 12.45790043
1415677_at 10.11306458
1415678_at 10.99434124
1415679_at 11.84619353
1415680_at 10.18849114
1415681_at 10.68218355
1415682_at 9.191055025
1415683_at 11.62783979
1415684_at 10.20041496
1415685_at 9.725003692
1415686_at 11.3750726
1415687_a_at 14.88096348
1415688_at 10.61340313
1415689_s_at 8.951170469

Total number of rows: 45101

Table truncated, full table size 1026 Kbytes.

Supplementary file Size Download File type/resource
GSM868304.CEL.gz 4.3 Mb (ftp)(http) CEL
Raw data provided as supplementary file
Processed data included within Sample table

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