|
| Status |
Public on Aug 17, 2012 |
| Title |
Human embryonic stem cell line T3ES, biological rep2 |
| Sample type |
RNA |
| |
|
| Source name |
GSM239825 from GSE9440
|
| Organism |
Homo sapiens |
| Characteristics |
cell type: ESC
|
| Growth protocol |
PMID: 21169409 PMID: 18612434
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip HG-U133_Plus_2 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450 (EukGE-WS2v5_450).
|
| Scan protocol |
GeneChips were scanned using the Scanner GSC3000 M10 type.
|
| Description |
Gene expression data of human embryonic stem cell
|
| Data processing |
justRMA( ) from and quantile normalization; standardized using 'Mfuzz'
|
| |
|
| Submission date |
Feb 07, 2012 |
| Last update date |
Aug 17, 2012 |
| Contact name |
YAU-HUA YU |
| E-mail(s) |
yauhuayu@gmail.com
|
| Organization name |
Tufts Univ. School of Dental Medicine
|
| Street address |
1 Kneeland Street
|
| City |
Boston |
| State/province |
Massachusetts |
| ZIP/Postal code |
02111 |
| Country |
USA |
| |
|
| Platform ID |
GPL570 |
| Series (1) |
| GSE35603 |
Network Biology of Tumor Stem-like Cells Identified a Regulatory Role of CBX5 in Lung Cancer |
|
| Relations |
| Reanalysis of |
GSM239825 |
