|
| Status |
Public on Apr 25, 2012 |
| Title |
STAT5 double knocked in IL-2 6 h rep 4 |
| Sample type |
RNA |
| |
|
| Source name |
T cell
|
| Organism |
Mus musculus |
| Characteristics |
genotype: STAT5 double knocked in
cell type: T cell
treatment: IL-2
time: 6 h
|
| Treatment protocol |
Not treated or treated with 100 U/ml of IL-2 for 2, 6, or 17 hr
|
| Growth protocol |
Pre-activated splenic T cells (5x1e+6) were rested overnight
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted from cultured EPC with an RNAqueous micro RNA isolation kit (Ambion, Austin, TX). Cells were lysed in buffer containing guanidinium thiocyanate.
|
| Label |
biotin
|
| Label protocol |
Biotin labeled with Affymetrix's IVT labeling kits (Affymetrix, Santa Clara, CA).
|
| |
|
| Hybridization protocol |
Fragmented RNA was assessed for relative length on Agilent 2100 bioanalyzer and hybridized to Affymetrix Mouse 430 2.0 chips for 16 hours.
|
| Scan protocol |
Affymetrix GeneChip scanner
|
| Description |
6h, double knocked in
STAT5 double knocked in
|
| Data processing |
Intensities were pre-processed with MAS5.0, and further processed with variance stablization transformation (S10)
|
| |
|
| Submission date |
Mar 28, 2012 |
| Last update date |
Apr 25, 2012 |
| Contact name |
Peng Li |
| E-mail(s) |
peng.li@nih.gov
|
| Organization name |
NIH
|
| Department |
NHLBI
|
| Lab |
LMI
|
| Street address |
9000 Rockville Pike
|
| City |
Bethesda |
| State/province |
MD |
| ZIP/Postal code |
20892 |
| Country |
USA |
| |
|
| Platform ID |
GPL1261 |
| Series (2) |
| GSE36888 |
Critical Role of STAT5 Transcription Factor Tetramerization for Cytokine Responses and Normal Immune Function (RNA) |
| GSE36890 |
Critical Role of STAT5 Transcription Factor Tetramerization for Cytokine Responses and Normal Immune Function |
|
