|
Status |
Public on Apr 25, 2012 |
Title |
STAT5 double knocked in IL-2 17 h rep 4 |
Sample type |
RNA |
|
|
Source name |
T cell
|
Organism |
Mus musculus |
Characteristics |
genotype: STAT5 double knocked in cell type: T cell treatment: IL-2 time: 17 h
|
Treatment protocol |
Not treated or treated with 100 U/ml of IL-2 for 2, 6, or 17 hr
|
Growth protocol |
Pre-activated splenic T cells (5x1e+6) were rested overnight
|
Extracted molecule |
total RNA |
Extraction protocol |
Total RNA was extracted from cultured EPC with an RNAqueous micro RNA isolation kit (Ambion, Austin, TX). Cells were lysed in buffer containing guanidinium thiocyanate.
|
Label |
biotin
|
Label protocol |
Biotin labeled with Affymetrix's IVT labeling kits (Affymetrix, Santa Clara, CA).
|
|
|
Hybridization protocol |
Fragmented RNA was assessed for relative length on Agilent 2100 bioanalyzer and hybridized to Affymetrix Mouse 430 2.0 chips for 16 hours.
|
Scan protocol |
Affymetrix GeneChip scanner
|
Description |
17h, double knocked in STAT5 double knocked in
|
Data processing |
Intensities were pre-processed with MAS5.0, and further processed with variance stablization transformation (S10)
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|
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Submission date |
Mar 28, 2012 |
Last update date |
Apr 25, 2012 |
Contact name |
Peng Li |
E-mail(s) |
peng.li@nih.gov
|
Organization name |
NIH
|
Department |
NHLBI
|
Lab |
LMI
|
Street address |
9000 Rockville Pike
|
City |
Bethesda |
State/province |
MD |
ZIP/Postal code |
20892 |
Country |
USA |
|
|
Platform ID |
GPL1261 |
Series (2) |
GSE36888 |
Critical Role of STAT5 Transcription Factor Tetramerization for Cytokine Responses and Normal Immune Function (RNA) |
GSE36890 |
Critical Role of STAT5 Transcription Factor Tetramerization for Cytokine Responses and Normal Immune Function |
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