|
Status |
Public on Oct 04, 2012 |
Title |
Fetal-day0-B |
Sample type |
RNA |
|
|
Source name |
Fetal CD34+ HSPCs
|
Organism |
Homo sapiens |
Characteristics |
tissue: primary HSPCs isolated from 2-trimester fetal liver developmental stage: 2-trimester fetal liver
|
Growth protocol |
Primary maturing fetal or adult erythroblasts were generated ex vivo using a serum-free two-phase liquid culture system.
|
Extracted molecule |
total RNA |
Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
Label |
biotin
|
Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol.
|
|
|
Hybridization protocol |
Standard Affymetrix protocol.
|
Scan protocol |
Standard Affymetrix protocol.
|
Description |
Ex vivo erythroid differentiation of magnetically-sorted CD34+ HSPCs
|
Data processing |
We RMA-normalized the arrays using the affy package from Bioconductor in R, and further eliminated batch effects using ComBat.
|
|
|
Submission date |
Apr 02, 2012 |
Last update date |
Oct 04, 2012 |
Contact name |
Jian Xu |
E-mail(s) |
Jian.Xu@UTSouthwestern.edu
|
Phone |
214-648-6125
|
Organization name |
UT Southwestern Medical Center
|
Department |
Children's Research Institute
|
Street address |
6000 Harry Hines Blvd. NL12.138B
|
City |
Dallas |
State/province |
TX |
ZIP/Postal code |
75235 |
Country |
USA |
|
|
Platform ID |
GPL15394 |
Series (2) |
GSE36984 |
Expression Profiling of Primary Human Fetal and Adult Hematopoietic Stem/Progenitor Cells (HSPCs) and Differentiating Proerythroblasts (ProEs) |
GSE36994 |
Comparative profiling of human fetal and adult erythropoiesis |
|