|
| Status |
Public on May 08, 2014 |
| Title |
WTp53, exp 4 |
| Sample type |
RNA |
| |
|
| Source name |
H1299_WTp53
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: NCI-H1299
cell type: Null p53, non-small cell lung carcinoma cells
transfected with: WTp53 in GFP vectors (pIRES-2EGFP)
|
| Treatment protocol |
ΔNp53:WTp53 or WTp53 in GFP vectors with Lipofectamin 2000 were used for transient transfection.
|
| Growth protocol |
Null p53 cell lines NCI-H1299 was cultured with 10% FBS supplemented RPMI-1640 till 95% confluence
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted with RNAeasy kit from trypsinized cells 40 hours after transfection.
|
| Label |
Biotin
|
| Label protocol |
cDNA/cRNA/cDNA was generated with the Ambion WT Expression kit and biotin labelled with GeneChip WT Terminal Labeling Kit
|
| |
|
| Hybridization protocol |
each sample was hybridized to Affymetrix GeneChip Human Gene 1.0 ST array
|
| Scan protocol |
examination with an Affymetrix GeneChip Scanner 3000
|
| Description |
Taatjes_WTG_4th
Gene expression data from transfected cell, 40hours after transfection
|
| Data processing |
Normalized RMA sketch were calculated by Expression Console (64bit, v1.1)
|
| |
|
| Submission date |
Apr 13, 2012 |
| Last update date |
May 08, 2014 |
| Contact name |
Shih-chieh Lin |
| Organization name |
University of Colorado at Boulder
|
| Department |
Chemistry and Biochemistry
|
| Lab |
Dr. Dylan Taatjes
|
| Street address |
215 UCB
|
| City |
Boulder |
| State/province |
CO |
| ZIP/Postal code |
80309 |
| Country |
USA |
| |
|
| Platform ID |
GPL6244 |
| Series (1) |
| GSE37271 |
Metabolic and gene expression changes induced by the naturally occurring ΔNp53 isoform link mTOR pathway and mitochondrial alterations to the progeroid phenotype. |
|
