|
| Status |
Public on Apr 28, 2012 |
| Title |
CTRL(F) |
| Sample type |
RNA |
| |
|
| Source name |
Trophoblast cells from human first-trimester placenta
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: HTR-8/Svneo
tranfected: control siRNA
|
| Treatment protocol |
HTR-8/SVneo cells were transfected with SPARC siRNA or a 25-nucleotide universal negative control siRNA using Lipofectamine 2000 (Invitrogen) according to the manufacturer’s protocol. Seventy-two hours after transfection, cells were harvested.
|
| Growth protocol |
HTR-8/SVneo cells were routinely maintained in RPMI1640 medium containing 10% fetal bovine serum (FBS), 1 mM sodium pyruvate, 2 mM L-glutamine, 100 μg/ml streptomycin, and 100 U/ml penicillin.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted with Trizol reagent, followed by clean-up and DNase I treatment with QIAGEN RNeasy mini kit in accordance with the prescribed protocol provided with the kit. Quality control was performed with Agilent Bioanalyser.
|
| Label |
Biotin
|
| Label protocol |
Biotinylated cRNA were prepared with the Ambion MessageAmp kit for Illumina arrays
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol
|
| Scan protocol |
Standard Illumina scanning protocol
|
| Description |
Cells transfected with control siRNA
|
| Data processing |
Total RNA extracted from HTR-8/SVneo cells was genotyped using Human WG-6 v3 BeadChips (Illumina).
|
| |
|
| Submission date |
Apr 27, 2012 |
| Last update date |
Apr 28, 2012 |
| Contact name |
Yahong Jiang |
| E-mail(s) |
yahongjiang@gmail.com
|
| Phone |
86-21-64437629
|
| Organization name |
Shanghai Institute of Planned Parenthood Research
|
| Street address |
2140 Xietu Road
|
| City |
Shanghai |
| ZIP/Postal code |
200032 |
| Country |
China |
| |
|
| Platform ID |
GPL6884 |
| Series (1) |
| GSE37639 |
Genome-wide analysis of SPARC(secreted protein acidic and rich in cysteine)-responsive gene expression in HTR-8/SVneo cells |
|
