|
| Status |
Public on Dec 30, 2012 |
| Title |
Curcumin_2 / Control_2 biol. Rep. 2; tech. Rep. 1 |
| Sample type |
RNA |
| |
|
| Channel 1 |
| Source name |
Curcumin_Treated_Adult_worms_sample_2
|
| Organism |
Schistosoma mansoni |
| Characteristics |
tissue: Adult worms
treatment: culture with Curcumin (15 μM ) for 24 h
|
| Treatment protocol |
Adult worms were kept in culture for 24 h with Curcumin (15uM) or the vehicle solution (DMSO)
|
| Growth protocol |
Adult worms were obtained by portal perfusion of 56-days infected hamsters
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA from all samples was extract using Trizol reagent (Invitrogen) followed by DNase treatment using RNeasy kit (QIAGEN) according to manufacturer´s instructions.
|
| Label |
Cy3
|
| Label protocol |
All samples were linearly amplified and labeled using Agilent protocol with Low input RNA amplification kit
|
| |
|
| Channel 2 |
| Source name |
Control_Adult_worms_sample_2
|
| Organism |
Schistosoma mansoni |
| Characteristics |
tissue: Adult worms
treatment: culture with DMSO (Non-treated) for 24 h
|
| Treatment protocol |
Adult worms were kept in culture for 24 h with Curcumin (15uM) or the vehicle solution (DMSO)
|
| Growth protocol |
Adult worms were obtained by portal perfusion of 56-days infected hamsters
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA from all samples was extract using Trizol reagent (Invitrogen) followed by DNase treatment using RNeasy kit (QIAGEN) according to manufacturer´s instructions.
|
| Label |
Cy5
|
| Label protocol |
All samples were linearly amplified and labeled using Agilent protocol with Low input RNA amplification kit
|
| |
|
| |
| Hybridization protocol |
Samples were combined and hybridized to a custom oligoarray with 4 x 44k elements manufactured by Agilent Technologies (GPL8606). Hybridization and washing steps were performed according to Agilent instructions.
|
| Scan protocol |
The washed slides were scanned using GenePix4000B (Molecular Devices) scanner.
|
| Data processing |
Data was extracted using Feature Extraction software (Agilent Technologies), the background subtracted intensities (gProcessedSignal and rProcessedSignal) were LOWESS normalized. Log2 ratio (Treated/Control) intensities was calculated.
|
| |
|
| Submission date |
Apr 29, 2012 |
| Last update date |
Dec 30, 2012 |
| Contact name |
Katia Cristina Pereira Oliveira |
| Organization name |
Instituto Adolfo Lutz
|
| Department |
Centro de Parasitologia e Micologia
|
| Lab |
Nucleo de Enterparasitas
|
| Street address |
Av Prof lineu prestes 748
|
| City |
São Paulo |
| State/province |
O |
| ZIP/Postal code |
05508000 |
| Country |
Brazil |
| |
|
| Platform ID |
GPL8606 |
| Series (1) |
| GSE37647 |
Effect of Curcumin on expression profile of Schistosoma mansoni adult worms |
|
