|
| Status |
Public on Dec 26, 2012 |
| Title |
Partially ligated left carotid artery Replicate 1 |
| Sample type |
RNA |
| |
|
| Source name |
Mouse
|
| Organism |
Mus musculus |
| Characteristics |
strain: apoE-/-
age: 6-8 weeks
Sex: Female
tissue: Left carotid artery
|
| Treatment protocol |
Partial Ligation: the left external carotid, the internal carotid and the occipital artery were ligated allowing blood flow only via the superior thyroid artery.
|
| Growth protocol |
6 weeks on high fat diet (21% crude fat, 0.15% cholesterol and 19.5% casein)
|
| Extracted molecule |
total RNA |
| Extraction protocol |
mirVana miRNA Isolation Kit, see http://www.ambion.com/catalog/CatNum.php?1560
|
| Label |
N/A
|
| Label protocol |
N/A
|
| |
|
| Hybridization protocol |
N/A
|
| Scan protocol |
N/A
|
| Description |
Biological replicate 1 of 6. Atherosclerotic left carotid artery, partially ligated.
|
| Data processing |
Taqman low density arrays were run on the 7900 HT Sequence Detection system. The ABI TaqMan SDS v2.3 software was utilized to obtain raw CT values. To review results, the raw CT data (SDS file format) were exported from the Plate Centric View into the ABI TaqMan RQ manager software. Automatic baseline and manual CTs were calculated with the RQ Manager Software for all samples. Statistical analysis: The global data set of miRs from card A and B was used for analysis. Data were calculated using Real Time StatMiner Tools [Integromics. http://www.integromics.com/StatMiner.php]. Input data for analysis consisted of all samples. CT values were normalized to the most stable endogenous controls (snoRNA135 and snoRNA202 for Card A and B) according to the Genorm algorithm.
|
| |
|
| Submission date |
May 15, 2012 |
| Last update date |
Dec 26, 2012 |
| Contact name |
Andreas Schober |
| E-mail(s) |
aschober@med.lmu.de
|
| Organization name |
Ludwig-Maximilians-University
|
| Department |
Institute for Cardiovascular Prevention
|
| Street address |
Pettenkoferstr. 9
|
| City |
Munich |
| State/province |
Bavaria |
| ZIP/Postal code |
80336 |
| Country |
Germany |
| |
|
| Platform ID |
GPL11419 |
| Series (2) |
| GSE26555 |
Changes in miR expression profile carotid plaques induced by acutely induced flow disturbances in apoe-/- mice on a high fat diet. |
| GSE26731 |
MicroRNA signature in murine atherosclerosis |
|
