tissue: pancreas cell type: Beta cells sample type: Test
Extracted molecule
total RNA
Extraction protocol
Total RNA was isolated using the miRVana microRNA isolation kit (Ambion).
Label
FAM
Label protocol
n/a
Hybridization protocol
n/a
Scan protocol
n/a
Description
Test
Data processing
Data was processed using the software SDS 2.3 and RQ manager 1.2 from Applied Biosystems. Cut off value for Ct was set in 32 cycles taking into account that these samples were preamplified. Therefore, undetermined expression and Ct values higher than 32 cycles were substituted by 32 for RQ calculation. If Cts in alpha and beta cell samples were > 32, the miRNA was considered as non-expressed. Ct for cycle threshold meaning the cycle in which the specific miRNA starts to be detected. ddCt for delta delta Ct, defined by the formula (Ct of the specific miRNA in the target sample-Ct of the endogenous control RNU48 in the target sample)-(Ct of the specific miRNA in the control sample-Ct of the endogenous control RNU48 in the control sample) for each human pancreas preparation. RQ for relative quantification, defined by the formula 2^-ddCt . Small nuclear RNA (RNU48) was used as endogenous control.
