|
| Status |
Public on Jun 03, 2012 |
| Title |
CbfA mutant_rep3 |
| Sample type |
SRA |
| |
|
| Source name |
CbfA mutant
|
| Organism |
Dictyostelium discoideum |
| Characteristics |
strain: JH.D
genotype/variation: CbfA mutant
|
| Treatment protocol |
Cells stored as dry pellets frozen at -80°C until RNA preparation
|
| Growth protocol |
Cells grown in liquid medium (HL-5)
|
| Extracted molecule |
polyA RNA |
| Extraction protocol |
Samples were processed using Illumina’s TruSeq™ RNA Sample Prep Kit v2 following the manufacturer’s instructions (RS-122-2001/2002)
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina HiSeq 2000 |
| |
|
| Description |
JH.D_rep3
|
| Data processing |
HCS v1.5.15.1
Reads were mapped to the Dictyostelium discoideum reference transcriptome downloaded from dictyBase (http://dictybase.org/; version: 2012-Jan-10) using the ELANDv2e (CASAVA v1.8)
RPKM values were calculated based on ELAND mapping output
Genome_build: http://dictybase.org/; version: 2012-Jan-10
Supplementary_files_format_and_content: txt format, RPKMs of all samples
|
| |
|
| Submission date |
Jun 01, 2012 |
| Last update date |
May 15, 2019 |
| Contact name |
Marco Groth |
| E-mail(s) |
dnaseq@leibniz-fli.de
|
| Organization name |
Leibniz Institute on Aging - FLI
|
| Department |
Core Facility - Next Generation Sequencing
|
| Street address |
Beutenbergstraße 11
|
| City |
Jena |
| ZIP/Postal code |
07747 |
| Country |
Germany |
| |
|
| Platform ID |
GPL15643 |
| Series (1) |
| GSE38418 |
Deeply conserved gene-regulatory functions of carboxy-terminal domains in dictyostelid C-module-binding factors |
|
| Relations |
| SRA |
SRX151301 |
| BioSample |
SAMN01036565 |
