|
Status |
Public on Jun 03, 2012 |
Title |
CbfA mutant expressing DdCbfA-CTD_rep3 |
Sample type |
SRA |
|
|
Source name |
CbfA mutant expressing DdCbfA-CTD
|
Organism |
Dictyostelium discoideum |
Characteristics |
strain: JH.D genotype/variation: CbfA mutant expressing D. discoideum CbfA-CTD
|
Treatment protocol |
Cells stored as dry pellets frozen at -80°C until RNA preparation
|
Growth protocol |
Cells grown in liquid medium (HL-5)
|
Extracted molecule |
polyA RNA |
Extraction protocol |
Samples were processed using Illumina’s TruSeq™ RNA Sample Prep Kit v2 following the manufacturer’s instructions (RS-122-2001/2002)
|
|
|
Library strategy |
RNA-Seq |
Library source |
transcriptomic |
Library selection |
cDNA |
Instrument model |
Illumina HiSeq 2000 |
|
|
Description |
JH.D[DdCTD]_rep3
|
Data processing |
HCS v1.5.15.1 Reads were mapped to the Dictyostelium discoideum reference transcriptome downloaded from dictyBase (http://dictybase.org/; version: 2012-Jan-10) using the ELANDv2e (CASAVA v1.8) RPKM values were calculated based on ELAND mapping output Genome_build: http://dictybase.org/; version: 2012-Jan-10 Supplementary_files_format_and_content: txt format, RPKMs of all samples
|
|
|
Submission date |
Jun 01, 2012 |
Last update date |
May 15, 2019 |
Contact name |
Marco Groth |
E-mail(s) |
dnaseq@leibniz-fli.de
|
Organization name |
Leibniz Institute on Aging - FLI
|
Department |
Core Facility - Next Generation Sequencing
|
Street address |
Beutenbergstraße 11
|
City |
Jena |
ZIP/Postal code |
07747 |
Country |
Germany |
|
|
Platform ID |
GPL15643 |
Series (1) |
GSE38418 |
Deeply conserved gene-regulatory functions of carboxy-terminal domains in dictyostelid C-module-binding factors |
|
Relations |
SRA |
SRX151304 |
BioSample |
SAMN01036568 |