|
| Status |
Public on Jan 29, 2013 |
| Title |
mock 1 |
| Sample type |
RNA |
| |
|
| Source name |
HEK293T cells
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: HEK293T
transfection molecule: mock
pull-down: none
|
| Treatment protocol |
200pmols of synthetic biotin-labeled miRNA duplexes were transfected into HEK293T cells using HiPerFect Transfection Reagent (Qiagen).
|
| Growth protocol |
HEK293T cells were grown in DMEM according to ATCC recommendations.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Cells were harvested 24 hours post transfection, and the miRNAs and their bound targets were isolated using streptavidin myOne C1 Dynabeads. Beads were washed before RNA extraction using an RNeasy Kit (Qiagen) according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared with the Ambion MessageAmp kit for Illumina arrays
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol
|
| Scan protocol |
Standard Illumina scanning protocol
|
| Description |
replicate 1
|
| Data processing |
The data were preprocessed by Bioconductor lumi package (version 2.2.1). It was background adjusted, vst transformed and rsn normalized. Probes that did not have a detection p-value < 0.01 in all of the mock-transfected replicates were excluded from further analysis.
|
| |
|
| Submission date |
Jun 08, 2012 |
| Last update date |
Jan 29, 2013 |
| Contact name |
Nicole Cloonan |
| E-mail(s) |
nicole.cloonan@qimrberghofer.edu.au
|
| Organization name |
QIMR Berghofer Medical Research Institute
|
| Lab |
Genomic Biology
|
| Street address |
300 Herston Road
|
| City |
Herston |
| State/province |
Queensland |
| ZIP/Postal code |
4006 |
| Country |
Australia |
| |
|
| Platform ID |
GPL6947 |
| Series (1) |
| GSE38593 |
MicroRNA-182-5p targets a network of genes involved in DNA repair |
|
