|
| Status |
Public on Jul 30, 2012 |
| Title |
TLR3p_IL1b_2h |
| Sample type |
RNA |
| |
|
| Source name |
Fibroblasts from patient with partial TLR3 deficiency
|
| Organism |
Homo sapiens |
| Characteristics |
gender: female
stimulation: IL1b
concentration: 20ng/ml
timepoints: 2h
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Cells were lysed in RLT + 1% β-Mercaptoethanol, RNA were isolated using Qiagen Rneasy mini kit
|
| Label |
Biotin
|
| Label protocol |
Biotinylated cRNA were prepared with the Ambion MessageAmp kit for Illumina arrays
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol
|
| Scan protocol |
Standard Illumina scanning protocol
|
| Description |
6142069027_D
|
| Data processing |
The data were normalised using quantile normalisation with in R. PALO (p<0.05 present in at least one) were used to filter the data.
|
| |
|
| Submission date |
Jun 12, 2012 |
| Last update date |
Jan 12, 2017 |
| Contact name |
Elisabeth Israelsson |
| E-mail(s) |
elisabeth.israelsson@astrazeneca.com
|
| Organization name |
AstraZeneca, IMED RIA
|
| Department |
Translational Biology
|
| Street address |
Pepparedsleden 1
|
| City |
Molndal |
| ZIP/Postal code |
SE-431 83 |
| Country |
Sweden |
| |
|
| Platform ID |
GPL10558 |
| Series (1) |
| GSE38652 |
In vitro responses of fibroblasts from patients with TBK1 deficiency after TLR3 dependent and independent stimuli |
|
