|
| Status |
Public on Jul 03, 2013 |
| Title |
volar epidermis from Sole rep1 |
| Sample type |
RNA |
| |
|
| Source name |
Epidermis from punch biopsy
|
| Organism |
Homo sapiens |
| Characteristics |
volar or non-volar: volar
upper or lower extremity: lower
tissue: volar epidermis from Sole
|
| Treatment protocol |
no treatments
|
| Growth protocol |
after biopsy, skin was treated with dipsase to isolate epidermis. Epidermal samples were stored in RNAlater prior to RNA extraction. No growth.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Qiagen Fibrous RNA kit
|
| Label |
biotin
|
| Label protocol |
One round amplification protocol for total RNA following Affymetrix’ specifications using T7 promoter engineered random primers for cDNA synthesis, and Ambion® WT Expression Kit (www.affymetrix.com).
|
| |
|
| Hybridization protocol |
16hrs at 45° C with rotation (60rpm) as described by Affymetrix in their GeneChip® Expression Wash, Stain and Scan User Manual (www.affymetrix.com).
|
| Scan protocol |
Using Affymetrix’ GeneChip Scanner 3000 7G and default parameters described by the manufacturer in their GeneChip Expression Analysis Technical Manual
|
| Data processing |
Image analysis used the Affymetrix Command Console version 3 (AGCC v3.0) software, processed with the Expression Console PLIER algorithm and the manufacturer’s specifications.
HuEx-1_0-st-v2.r2.pgf
HuEx-1_0-st-v2.na31.hg19.probeset_extended.mps
|
| |
|
| Submission date |
Jul 11, 2012 |
| Last update date |
Jul 03, 2013 |
| Contact name |
Luis Andres Garza |
| E-mail(s) |
LAG@jhmi.edu
|
| Phone |
410-955-8662
|
| Organization name |
Johns Hopkins School of Medicine
|
| Department |
Dermatology
|
| Lab |
Garza lab
|
| Street address |
1550 Orleans St. Suite 204 CRBII Koch
|
| City |
Baltimore |
| State/province |
MD |
| ZIP/Postal code |
21287 |
| Country |
USA |
| |
|
| Platform ID |
GPL5175 |
| Series (1) |
| GSE39267 |
Stump project -- mRNA epidermis; Volar versus non-volar acral skin gene expression |
|
