|
| Status |
Public on Dec 20, 2012 |
| Title |
ieSCs (from MEFs)-1 (YB2) |
| Sample type |
RNA |
| |
|
| Source name |
ieSCs (from MEFs)-1
|
| Organism |
Mus musculus |
| Characteristics |
background strain: Congenic, homo R26-M2rtTA strain
cell type: embryonic Sertoli-like cells
transgene: Nr5a1, Wt1, Dmrt1, Gata4, Sox9
|
| Growth protocol |
Cells were grown 2 weeks in the absent of dox in F12/DMEM medium in 20% O2 and 5% CO2
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Qiagen Rneasy kit Cat-74106
|
| Label |
Cy3
|
| Label protocol |
Standard Agilent Protocol
|
| |
|
| Hybridization protocol |
Standard Agilent Protocol
|
| Scan protocol |
Standard Agilent Protocol
|
| Description |
gene expression after 2 weeks of dox removal
YB2
|
| Data processing |
Quantile Normalization using limma R package
|
| |
|
| Submission date |
Jul 25, 2012 |
| Last update date |
Dec 20, 2012 |
| Contact name |
Albert W Cheng |
| E-mail(s) |
awcheng@mit.edu
|
| Organization name |
Whitehead Institute
|
| Street address |
Room 453, 9 Cambridge Center
|
| City |
Cambridge |
| State/province |
MA |
| ZIP/Postal code |
02139 |
| Country |
USA |
| |
|
| Platform ID |
GPL13912 |
| Series (2) |
| GSE35653 |
Direct reprogramming of fibroblasts into embryonic Sertoli-like cells by defined factors |
| GSE39650 |
Comparison of gene expression between MEFs and induced embryonic Sertoli-like cells |
|
