|
| Status |
Public on Sep 06, 2012 |
| Title |
Mek\NestinCre mutant dorsal cortex_E18.5_rep2 |
| Sample type |
RNA |
| |
|
| Source name |
E18.5 dorsal cortex
|
| Organism |
Mus musculus |
| Characteristics |
tissue: dorsal cortex
genotype/variation: Mek1fl/fl, Mek2-/- ,NestinCre
age: E18.5
|
| Treatment protocol |
no treatment were applied before extraction of RNA
|
| Growth protocol |
Tissue were directly taken from animals
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted using Trizol (Invitrogen) and purified using RNAeasy plus mini kit (QIAGEN).
|
| Label |
biotin
|
| Label protocol |
RNA were labeled using standard GeneChip®WT Terminal labeling protocal from Affymetrix
|
| |
|
| Hybridization protocol |
Hybridization were carried out according to GeneChip®WT hybridization mannual from Affymetrix
|
| Scan protocol |
The arrays were scanned with the Affymetrix GeneChip Scanner 3000 7G Plus
|
| Data processing |
The data were analyzed with Partex Genomics Suite 6.6. The gene level RMA-sketch algorithm was used to generate the intensity data for the Mouse Gene 1.0 ST arrays. Differences in gene expression were expressed as fold changes (down-regulated genes are expressed as -fold value). Fold-change was calculated based on log2 ratio. Transcripts down-regulated >-2 fold were considered interesting candidates and some were further validated with IHC and western blotting.
|
| |
|
| Submission date |
Sep 06, 2012 |
| Last update date |
Sep 06, 2012 |
| Contact name |
xiaoyan li |
| E-mail(s) |
xiaoyan_li@med.unc.edu
|
| Organization name |
University of North Carolina
|
| Department |
Neuroscience Center
|
| Lab |
Dr. William Snider lab
|
| Street address |
115 Mason Farm Road
|
| City |
Chapel Hill |
| State/province |
NC |
| ZIP/Postal code |
27599 |
| Country |
USA |
| |
|
| Platform ID |
GPL6246 |
| Series (1) |
| GSE40675 |
Expression data from E18.5 mouse dorsal telencephalon |
|
