Mcf2l MCF.2 cell line derived transforming sequence-like [Rattus norvegicus (Norway rat)] - Gene

Summary

Symbol: Mcf2lprovided by RGD
Full Name: MCF.2 cell line derived transforming sequence-likeprovided by RGD
Primary source: RGD:619782
See related: Ensembl:ENSRNOG00000028426 AllianceGenome:RGD:619782
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Rattus norvegicus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Rattus
Also known as: Ost; mcf 2l
Summary: Enables guanyl-nucleotide exchange factor activity. Involved in positive regulation of Rho protein signal transduction. Located in cytoplasm. Is extrinsic component of membrane. Orthologous to human MCF2L (MCF.2 cell line derived transforming sequence like). [provided by Alliance of Genome Resources, Apr 2022]
Expression: Biased expression in Brain (RPKM 86.9), Kidney (RPKM 46.2) and 8 other tissues See more
Orthologs: human mouse all
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Genomic context

Location:: 16q12.5

Exon count:: 38

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCr8 (GCF_036323735.1)	16	NC_086034.1 (83209277..83355683, complement)
RS_2023_06	previous assembly	mRatBN7.2 (GCF_015227675.2)	16	NC_051351.1 (76507133..76652893, complement)
106	previous assembly	Rnor_6.0 (GCF_000001895.5)	16	NC_005115.4 (81839686..81986007, complement)

Chromosome 16 - NC_086034.1 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: A rat RNA-Seq transcriptomic BodyMap across 11 organs and 4 developmental stages
Description: 320 RNA samples isolated from 11 organs (adrenal gland, brain, heart, kidney, liver, lung, muscle, spleen, thymus, and testes or uterus) from both sexes of Fischer 344 rats across four developmental stages (2-, 6-, 21-, and 104-weeks-old)
BioProject: PRJNA238328
Publication: PMID 24510058
Analysis date: Mon Jun 6 17:44:12 2016

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

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General gene information

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Gene Ontology Provided by RGD

Function	Evidence Code	Pubs
enables 1-phosphatidylinositol binding	ISO Inferred from Sequence Orthology more info
enables guanyl-nucleotide exchange factor activity	IBA Inferred from Biological aspect of Ancestor more info
enables guanyl-nucleotide exchange factor activity	IDA Inferred from Direct Assay more info	PubMed
enables guanyl-nucleotide exchange factor activity	IEA Inferred from Electronic Annotation more info
enables guanyl-nucleotide exchange factor activity	ISO Inferred from Sequence Orthology more info
enables phosphatidylinositol binding	IEA Inferred from Electronic Annotation more info
enables phosphatidylinositol binding	ISO Inferred from Sequence Orthology more info

Process	Evidence Code	Pubs
acts_upstream_of_or_within Rho protein signal transduction	ISO Inferred from Sequence Orthology more info
involved_in intracellular signal transduction	IDA Inferred from Direct Assay more info	PubMed
involved_in intracellular signal transduction	IEA Inferred from Electronic Annotation more info
involved_in positive regulation of Rho protein signal transduction	IBA Inferred from Biological aspect of Ancestor more info
involved_in positive regulation of Rho protein signal transduction	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within positive regulation of transcription by RNA polymerase II	ISO Inferred from Sequence Orthology more info

Component	Evidence Code	Pubs
is_active_in cytoplasm	IBA Inferred from Biological aspect of Ancestor more info
located_in cytoplasm	IDA Inferred from Direct Assay more info	PubMed
located_in cytoplasm	IEA Inferred from Electronic Annotation more info
located_in cytoplasm	ISO Inferred from Sequence Orthology more info
located_in endomembrane system	ISO Inferred from Sequence Orthology more info
located_in extrinsic component of cytoplasmic side of plasma membrane	ISO Inferred from Sequence Orthology more info
is_active_in extrinsic component of membrane	IBA Inferred from Biological aspect of Ancestor more info
located_in lamellipodium	ISO Inferred from Sequence Orthology more info
located_in membrane	ISO Inferred from Sequence Orthology more info
located_in plasma membrane	IEA Inferred from Electronic Annotation more info

General protein information

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Preferred Names: guanine nucleotide exchange factor DBS

Names: DBL's big sister; MCF2-transforming sequence-like protein; OST oncogene; mcf.2 transforming sequence-like

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_001413769.1 → NP_001400698.1 guanine nucleotide exchange factor DBS isoform 2

Status: VALIDATED

Source sequence(s)

JAXUCZ010000016
NM_053951.2 → NP_446403.2 guanine nucleotide exchange factor DBS isoform 1

See identical proteins and their annotated locations for NP_446403.2

Status: VALIDATED

Source sequence(s)

CH473970

UniProtKB/TrEMBL

A6IWK6, D4A7F3

Related

ENSRNOP00000023352.6, ENSRNOT00000023352.7

Conserved Domains (6) summary

cd11857
Location:1082 → 1134

SH3_DBS; Src homology 3 domain of DBL's Big Sister (DBS), a guanine nucleotide exchange factor

smart00516
Location:46 → 191

SEC14; Domain in homologues of a S. cerevisiae phosphatidylinositol transfer protein (Sec14p)

cd00160
Location:603 → 780

RhoGEF; Guanine nucleotide exchange factor for Rho/Rac/Cdc42-like GTPases; Also called Dbl-homologous (DH) domain. It appears that PH domains invariably occur C-terminal to RhoGEF/DH domains.

cd00176
Location:322 → 524

SPEC; Spectrin repeats, found in several proteins involved in cytoskeletal structure; family members include spectrin, alpha-actinin and dystrophin; the spectrin repeat forms a three helix bundle with the second helix interrupted by proline in some sequences; ...

cd01227
Location:787 → 918

PH_Dbs; DBL's big sister protein pleckstrin homology (PH) domain

pfam00169
Location:801 → 915

PH; PH domain

RefSeqs of Annotated Genomes: GCF_036323735.1-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCr8

Genomic

NC_086034.1 Reference GRCr8

Range

83209277..83355683 complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_039094165.2 → XP_038950093.1 guanine nucleotide exchange factor DBS isoform X8

UniProtKB/TrEMBL

A0A8I5Y6G8

Conserved Domains (5) summary

cd11857
Location:1115 → 1167

SH3_DBS; Src homology 3 domain of DBL's Big Sister (DBS), a guanine nucleotide exchange factor

smart00516
Location:76 → 221

SEC14; Domain in homologues of a S. cerevisiae phosphatidylinositol transfer protein (Sec14p)

cd00160
Location:633 → 810

RhoGEF; Guanine nucleotide exchange factor for Rho/Rac/Cdc42-like GTPases; Also called Dbl-homologous (DH) domain. It appears that PH domains invariably occur C-terminal to RhoGEF/DH domains.

cd00176
Location:352 → 554

SPEC; Spectrin repeats, found in several proteins involved in cytoskeletal structure; family members include spectrin, alpha-actinin and dystrophin; the spectrin repeat forms a three helix bundle with the second helix interrupted by proline in some sequences; ...

cd01227
Location:817 → 948

PH_Dbs; DBL's big sister protein pleckstrin homology (PH) domain
XM_039094166.2 → XP_038950094.1 guanine nucleotide exchange factor DBS isoform X12

UniProtKB/Swiss-Prot

Q63406

UniProtKB/TrEMBL

D4A7F3

Related

ENSRNOP00000068993.2

Conserved Domains (5) summary

cd11857
Location:1059 → 1111

SH3_DBS; Src homology 3 domain of DBL's Big Sister (DBS), a guanine nucleotide exchange factor

smart00516
Location:76 → 221

SEC14; Domain in homologues of a S. cerevisiae phosphatidylinositol transfer protein (Sec14p)

cd00160
Location:633 → 810

RhoGEF; Guanine nucleotide exchange factor for Rho/Rac/Cdc42-like GTPases; Also called Dbl-homologous (DH) domain. It appears that PH domains invariably occur C-terminal to RhoGEF/DH domains.

cd00176
Location:352 → 554

SPEC; Spectrin repeats, found in several proteins involved in cytoskeletal structure; family members include spectrin, alpha-actinin and dystrophin; the spectrin repeat forms a three helix bundle with the second helix interrupted by proline in some sequences; ...

cd01227
Location:817 → 948

PH_Dbs; DBL's big sister protein pleckstrin homology (PH) domain
XM_063275044.1 → XP_063131114.1 guanine nucleotide exchange factor DBS isoform X16
XM_017599979.3 → XP_017455468.1 guanine nucleotide exchange factor DBS isoform X11

UniProtKB/TrEMBL

A0A8I5Y6G8
XM_006253421.5 → XP_006253483.1 guanine nucleotide exchange factor DBS isoform X6

UniProtKB/TrEMBL

A0A8I5ZZZ7, D4A7F3

Related

ENSRNOP00000085354.1

Conserved Domains (6) summary

cd11857
Location:1128 → 1180

SH3_DBS; Src homology 3 domain of DBL's Big Sister (DBS), a guanine nucleotide exchange factor

smart00516
Location:145 → 290

SEC14; Domain in homologues of a S. cerevisiae phosphatidylinositol transfer protein (Sec14p)

cd00160
Location:702 → 879

RhoGEF; Guanine nucleotide exchange factor for Rho/Rac/Cdc42-like GTPases; Also called Dbl-homologous (DH) domain. It appears that PH domains invariably occur C-terminal to RhoGEF/DH domains.

cd00176
Location:421 → 623

SPEC; Spectrin repeats, found in several proteins involved in cytoskeletal structure; family members include spectrin, alpha-actinin and dystrophin; the spectrin repeat forms a three helix bundle with the second helix interrupted by proline in some sequences; ...

cd01227
Location:886 → 1017

PH_Dbs; DBL's big sister protein pleckstrin homology (PH) domain

pfam00169
Location:900 → 1014

PH; PH domain
XM_039094163.2 → XP_038950091.1 guanine nucleotide exchange factor DBS isoform X7

UniProtKB/TrEMBL

D4A7F3

Conserved Domains (5) summary

cd11857
Location:1128 → 1180

SH3_DBS; Src homology 3 domain of DBL's Big Sister (DBS), a guanine nucleotide exchange factor

smart00516
Location:145 → 290

SEC14; Domain in homologues of a S. cerevisiae phosphatidylinositol transfer protein (Sec14p)

cd00160
Location:702 → 879

RhoGEF; Guanine nucleotide exchange factor for Rho/Rac/Cdc42-like GTPases; Also called Dbl-homologous (DH) domain. It appears that PH domains invariably occur C-terminal to RhoGEF/DH domains.

cd00176
Location:421 → 623

SPEC; Spectrin repeats, found in several proteins involved in cytoskeletal structure; family members include spectrin, alpha-actinin and dystrophin; the spectrin repeat forms a three helix bundle with the second helix interrupted by proline in some sequences; ...

cd01227
Location:886 → 1017

PH_Dbs; DBL's big sister protein pleckstrin homology (PH) domain
XM_039094162.2 → XP_038950090.1 guanine nucleotide exchange factor DBS isoform X4

UniProtKB/TrEMBL

A0A8I5Y6G8, A0A8I5ZWU6

Related

ENSRNOP00000083374.1

Conserved Domains (5) summary

cd11857
Location:1153 → 1205

SH3_DBS; Src homology 3 domain of DBL's Big Sister (DBS), a guanine nucleotide exchange factor

smart00516
Location:145 → 290

SEC14; Domain in homologues of a S. cerevisiae phosphatidylinositol transfer protein (Sec14p)

cd00160
Location:702 → 879

RhoGEF; Guanine nucleotide exchange factor for Rho/Rac/Cdc42-like GTPases; Also called Dbl-homologous (DH) domain. It appears that PH domains invariably occur C-terminal to RhoGEF/DH domains.

cd00176
Location:421 → 623

SPEC; Spectrin repeats, found in several proteins involved in cytoskeletal structure; family members include spectrin, alpha-actinin and dystrophin; the spectrin repeat forms a three helix bundle with the second helix interrupted by proline in some sequences; ...

cd01227
Location:886 → 1017

PH_Dbs; DBL's big sister protein pleckstrin homology (PH) domain
XM_039094160.2 → XP_038950088.1 guanine nucleotide exchange factor DBS isoform X3

UniProtKB/TrEMBL

A0A8I5Y6G8

Conserved Domains (5) summary

cd11857
Location:1181 → 1233

SH3_DBS; Src homology 3 domain of DBL's Big Sister (DBS), a guanine nucleotide exchange factor

smart00516
Location:145 → 290

SEC14; Domain in homologues of a S. cerevisiae phosphatidylinositol transfer protein (Sec14p)

cd00160
Location:702 → 879

RhoGEF; Guanine nucleotide exchange factor for Rho/Rac/Cdc42-like GTPases; Also called Dbl-homologous (DH) domain. It appears that PH domains invariably occur C-terminal to RhoGEF/DH domains.

cd00176
Location:421 → 623

SPEC; Spectrin repeats, found in several proteins involved in cytoskeletal structure; family members include spectrin, alpha-actinin and dystrophin; the spectrin repeat forms a three helix bundle with the second helix interrupted by proline in some sequences; ...

cd01227
Location:886 → 1017

PH_Dbs; DBL's big sister protein pleckstrin homology (PH) domain
XM_006253416.5 → XP_006253478.1 guanine nucleotide exchange factor DBS isoform X1

UniProtKB/TrEMBL

A0A8I5Y6G8

Conserved Domains (6) summary

cd11857
Location:1184 → 1236

SH3_DBS; Src homology 3 domain of DBL's Big Sister (DBS), a guanine nucleotide exchange factor

smart00516
Location:145 → 290

SEC14; Domain in homologues of a S. cerevisiae phosphatidylinositol transfer protein (Sec14p)

cd00160
Location:702 → 879

RhoGEF; Guanine nucleotide exchange factor for Rho/Rac/Cdc42-like GTPases; Also called Dbl-homologous (DH) domain. It appears that PH domains invariably occur C-terminal to RhoGEF/DH domains.

cd00176
Location:421 → 623

SPEC; Spectrin repeats, found in several proteins involved in cytoskeletal structure; family members include spectrin, alpha-actinin and dystrophin; the spectrin repeat forms a three helix bundle with the second helix interrupted by proline in some sequences; ...

cd01227
Location:886 → 1017

PH_Dbs; DBL's big sister protein pleckstrin homology (PH) domain

pfam00169
Location:900 → 1014

PH; PH domain
XM_039094159.2 → XP_038950087.1 guanine nucleotide exchange factor DBS isoform X2

UniProtKB/TrEMBL

A0A8I5Y6G8

Conserved Domains (5) summary

cd11857
Location:1184 → 1236

SH3_DBS; Src homology 3 domain of DBL's Big Sister (DBS), a guanine nucleotide exchange factor

smart00516
Location:145 → 290

SEC14; Domain in homologues of a S. cerevisiae phosphatidylinositol transfer protein (Sec14p)

cd00160
Location:702 → 879

RhoGEF; Guanine nucleotide exchange factor for Rho/Rac/Cdc42-like GTPases; Also called Dbl-homologous (DH) domain. It appears that PH domains invariably occur C-terminal to RhoGEF/DH domains.

cd00176
Location:421 → 623

SPEC; Spectrin repeats, found in several proteins involved in cytoskeletal structure; family members include spectrin, alpha-actinin and dystrophin; the spectrin repeat forms a three helix bundle with the second helix interrupted by proline in some sequences; ...

cd01227
Location:886 → 1017

PH_Dbs; DBL's big sister protein pleckstrin homology (PH) domain
XM_006253423.5 → XP_006253485.1 guanine nucleotide exchange factor DBS isoform X9

UniProtKB/TrEMBL

A0A8I5Y6G8

Conserved Domains (6) summary

cd11857
Location:1089 → 1141

SH3_DBS; Src homology 3 domain of DBL's Big Sister (DBS), a guanine nucleotide exchange factor

smart00516
Location:50 → 195

SEC14; Domain in homologues of a S. cerevisiae phosphatidylinositol transfer protein (Sec14p)

cd00160
Location:607 → 784

RhoGEF; Guanine nucleotide exchange factor for Rho/Rac/Cdc42-like GTPases; Also called Dbl-homologous (DH) domain. It appears that PH domains invariably occur C-terminal to RhoGEF/DH domains.

cd00176
Location:326 → 528

SPEC; Spectrin repeats, found in several proteins involved in cytoskeletal structure; family members include spectrin, alpha-actinin and dystrophin; the spectrin repeat forms a three helix bundle with the second helix interrupted by proline in some sequences; ...

cd01227
Location:791 → 922

PH_Dbs; DBL's big sister protein pleckstrin homology (PH) domain

pfam00169
Location:805 → 919

PH; PH domain
XM_039094167.2 → XP_038950095.1 guanine nucleotide exchange factor DBS isoform X13

UniProtKB/TrEMBL

A0A8L2QMZ3, D4A7F3

Related

ENSRNOP00000047024.5

Conserved Domains (5) summary

cd11857
Location:1033 → 1085

SH3_DBS; Src homology 3 domain of DBL's Big Sister (DBS), a guanine nucleotide exchange factor

smart00516
Location:50 → 195

SEC14; Domain in homologues of a S. cerevisiae phosphatidylinositol transfer protein (Sec14p)

cd00160
Location:607 → 784

RhoGEF; Guanine nucleotide exchange factor for Rho/Rac/Cdc42-like GTPases; Also called Dbl-homologous (DH) domain. It appears that PH domains invariably occur C-terminal to RhoGEF/DH domains.

cd00176
Location:326 → 528

SPEC; Spectrin repeats, found in several proteins involved in cytoskeletal structure; family members include spectrin, alpha-actinin and dystrophin; the spectrin repeat forms a three helix bundle with the second helix interrupted by proline in some sequences; ...

cd01227
Location:791 → 922

PH_Dbs; DBL's big sister protein pleckstrin homology (PH) domain
XM_063275040.1 → XP_063131110.1 guanine nucleotide exchange factor DBS isoform X5
XM_006253424.5 → XP_006253486.1 guanine nucleotide exchange factor DBS isoform X10

UniProtKB/TrEMBL

A0A0G2KA20, A0A8I5Y6G8

Related

ENSRNOP00000075224.1

Conserved Domains (6) summary

cd11857
Location:1083 → 1135

SH3_DBS; Src homology 3 domain of DBL's Big Sister (DBS), a guanine nucleotide exchange factor

smart00516
Location:44 → 189

SEC14; Domain in homologues of a S. cerevisiae phosphatidylinositol transfer protein (Sec14p)

cd00160
Location:601 → 778

RhoGEF; Guanine nucleotide exchange factor for Rho/Rac/Cdc42-like GTPases; Also called Dbl-homologous (DH) domain. It appears that PH domains invariably occur C-terminal to RhoGEF/DH domains.

cd00176
Location:320 → 522

SPEC; Spectrin repeats, found in several proteins involved in cytoskeletal structure; family members include spectrin, alpha-actinin and dystrophin; the spectrin repeat forms a three helix bundle with the second helix interrupted by proline in some sequences; ...

cd01227
Location:785 → 916

PH_Dbs; DBL's big sister protein pleckstrin homology (PH) domain

pfam00169
Location:799 → 913

PH; PH domain
XM_063275042.1 → XP_063131112.1 guanine nucleotide exchange factor DBS isoform X15
XM_063275041.1 → XP_063131111.1 guanine nucleotide exchange factor DBS isoform X14
XM_039094169.2 → XP_038950097.1 guanine nucleotide exchange factor DBS isoform X17

UniProtKB/TrEMBL

A0A8I5Y6G8

Conserved Domains (5) summary

cd11857
Location:926 → 978

SH3_DBS; Src homology 3 domain of DBL's Big Sister (DBS), a guanine nucleotide exchange factor

cd00160
Location:444 → 621

RhoGEF; Guanine nucleotide exchange factor for Rho/Rac/Cdc42-like GTPases; Also called Dbl-homologous (DH) domain. It appears that PH domains invariably occur C-terminal to RhoGEF/DH domains.

cd00176
Location:163 → 365

SPEC; Spectrin repeats, found in several proteins involved in cytoskeletal structure; family members include spectrin, alpha-actinin and dystrophin; the spectrin repeat forms a three helix bundle with the second helix interrupted by proline in some sequences; ...

cd01227
Location:628 → 759

PH_Dbs; DBL's big sister protein pleckstrin homology (PH) domain

cl15787
Location:5 → 35

SEC14; Sec14p-like lipid-binding domain. Found in secretory proteins, such as S. cerevisiae phosphatidylinositol transfer protein (Sec14p), and in lipid regulated proteins such as RhoGAPs, RhoGEFs and neurofibromin (NF1). SEC14 domain of Dbl is known to ...