Aak1 AP2 associated kinase 1 [Rattus norvegicus (Norway rat)] - Gene

Summary

Official Symbol: Aak1provided by RGD
Official Full Name: AP2 associated kinase 1provided by RGD
Primary source: RGD:1305520
See related: Ensembl:ENSRNOG00000018317 AllianceGenome:RGD:1305520
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Rattus norvegicus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Rattus
Also known as: RGD1563580
Summary: Enables AP-2 adaptor complex binding activity and protein serine/threonine kinase activity. Involved in protein autophosphorylation and regulation of clathrin-dependent endocytosis. Located in cell leading edge and terminal bouton. Part of clathrin complex. Is active in presynapse. Orthologous to human AAK1 (AP2 associated kinase 1). [provided by Alliance of Genome Resources, Apr 2022]
Expression: Biased expression in Brain (RPKM 315.7), Adrenal (RPKM 93.7) and 9 other tissues See more
Orthologs: human mouse all
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Genomic context

Location:: 4q34

Exon count:: 23

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCr8 (GCF_036323735.1)	4	NC_086022.1 (120845012..121009170)
RS_2023_06	previous assembly	mRatBN7.2 (GCF_015227675.2)	4	NC_051339.1 (119300128..119451834)
106	previous assembly	Rnor_6.0 (GCF_000001895.5)	4	NC_005103.4 (118653851..118806796)

Chromosome 4 - NC_086022.1 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: A rat RNA-Seq transcriptomic BodyMap across 11 organs and 4 developmental stages
Description: 320 RNA samples isolated from 11 organs (adrenal gland, brain, heart, kidney, liver, lung, muscle, spleen, thymus, and testes or uterus) from both sexes of Fischer 344 rats across four developmental stages (2-, 6-, 21-, and 104-weeks-old)
BioProject: PRJNA238328
Publication: PMID 24510058
Analysis date: Mon Jun 6 17:44:12 2016

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

miRNA-384-3p alleviates sevoflurane-induced nerve injury by inhibiting Aak1 kinase in neonatal rats.
Title: miRNA-384-3p alleviates sevoflurane-induced nerve injury by inhibiting Aak1 kinase in neonatal rats.
This study demonistrated that AAK1 contributes to dendrite growth regulation.
Title: Chemical genetic identification of NDR1/2 kinase substrates AAK1 and Rabin8 Uncovers their roles in dendrite arborization and spine development.
AAK1 is a relevant target of K252a, that the loss of AAK1 alters ErbB4 trafficking and expression levels, providing evidence for a previously unrecognized role for AAK1 in Nrg1-mediated neurotrophic factor signaling.
Title: AAK1 identified as an inhibitor of neuregulin-1/ErbB4-dependent neurotrophic factor signaling using integrative chemical genomics and proteomics.

Submit: New GeneRIF Correction

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

RH132363 (e-PCR)
- UniSTS:215646

RH132368 (e-PCR)
- UniSTS:215651

RH143910 (e-PCR)
- UniSTS:233156

Gene Ontology Provided by RGD

Function	Evidence Code	Pubs
enables AP-2 adaptor complex binding	IBA Inferred from Biological aspect of Ancestor more info
enables AP-2 adaptor complex binding	IDA Inferred from Direct Assay more info	PubMed
enables AP-2 adaptor complex binding	IEA Inferred from Electronic Annotation more info
enables AP-2 adaptor complex binding	ISO Inferred from Sequence Orthology more info
enables ATP binding	IEA Inferred from Electronic Annotation more info
enables Notch binding	IEA Inferred from Electronic Annotation more info
enables Notch binding	ISO Inferred from Sequence Orthology more info
enables Notch binding	ISS Inferred from Sequence or Structural Similarity more info
enables protein kinase activity	IEA Inferred from Electronic Annotation more info
enables protein serine kinase activity	IEA Inferred from Electronic Annotation more info
enables protein serine/threonine kinase activity	IBA Inferred from Biological aspect of Ancestor more info
enables protein serine/threonine kinase activity	IDA Inferred from Direct Assay more info	PubMed

Process	Evidence Code	Pubs
involved_in positive regulation of Notch signaling pathway	IEA Inferred from Electronic Annotation more info
involved_in positive regulation of Notch signaling pathway	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of Notch signaling pathway	ISS Inferred from Sequence or Structural Similarity more info
involved_in presynaptic endocytosis	EXP Inferred from Experiment more info	PubMed
involved_in presynaptic endocytosis	IDA Inferred from Direct Assay more info	PubMed
involved_in presynaptic endocytosis	ISO Inferred from Sequence Orthology more info
involved_in protein phosphorylation	IBA Inferred from Biological aspect of Ancestor more info
involved_in protein stabilization	IEA Inferred from Electronic Annotation more info
involved_in protein stabilization	ISO Inferred from Sequence Orthology more info
involved_in protein stabilization	ISS Inferred from Sequence or Structural Similarity more info
involved_in regulation of clathrin-dependent endocytosis	IBA Inferred from Biological aspect of Ancestor more info
involved_in regulation of clathrin-dependent endocytosis	IDA Inferred from Direct Assay more info	PubMed
involved_in regulation of clathrin-dependent endocytosis	IEA Inferred from Electronic Annotation more info
involved_in regulation of clathrin-dependent endocytosis	ISO Inferred from Sequence Orthology more info
involved_in regulation of protein localization	IEA Inferred from Electronic Annotation more info
involved_in regulation of protein localization	ISO Inferred from Sequence Orthology more info
involved_in regulation of protein localization	ISS Inferred from Sequence or Structural Similarity more info

Component	Evidence Code	Pubs
is_active_in calyx of Held	EXP Inferred from Experiment more info	PubMed
is_active_in calyx of Held	IDA Inferred from Direct Assay more info	PubMed
located_in cell leading edge	IDA Inferred from Direct Assay more info	PubMed
part_of clathrin complex	IDA Inferred from Direct Assay more info	PubMed
located_in clathrin-coated pit	ISS Inferred from Sequence or Structural Similarity more info
located_in clathrin-coated vesicle	ISO Inferred from Sequence Orthology more info
located_in cytosol	IEA Inferred from Electronic Annotation more info
located_in cytosol	ISO Inferred from Sequence Orthology more info
located_in intracellular membrane-bounded organelle	IEA Inferred from Electronic Annotation more info
located_in intracellular membrane-bounded organelle	ISO Inferred from Sequence Orthology more info
located_in plasma membrane	IEA Inferred from Electronic Annotation more info
located_in plasma membrane	ISO Inferred from Sequence Orthology more info
is_active_in presynapse	IBA Inferred from Biological aspect of Ancestor more info
is_active_in presynapse	IDA Inferred from Direct Assay more info	PubMed
located_in presynapse	ISO Inferred from Sequence Orthology more info
located_in synapse	ISO Inferred from Sequence Orthology more info
located_in terminal bouton	IDA Inferred from Direct Assay more info	PubMed

General protein information

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Preferred Names: AP2-associated protein kinase 1

Names: adaptor-associated kinase 1

NP_001166921.1

EC 2.7.11.1

XP_006236895.1

EC 2.7.11.1

XP_006236896.1

EC 2.7.11.1

XP_017448306.1

EC 2.7.11.1

XP_017448307.1

EC 2.7.11.1

XP_017448308.1

EC 2.7.11.1

XP_017448309.1

EC 2.7.11.1

XP_017448310.1

EC 2.7.11.1

XP_017448311.1

EC 2.7.11.1

XP_038964087.1

EC 2.7.11.1

XP_038964088.1

EC 2.7.11.1

XP_038964089.1

EC 2.7.11.1

XP_038964090.1

EC 2.7.11.1

XP_063142600.1

EC 2.7.11.1

XP_063142601.1

EC 2.7.11.1

XP_063142602.1

EC 2.7.11.1

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_001173450.1 → NP_001166921.1 AP2-associated protein kinase 1

See identical proteins and their annotated locations for NP_001166921.1

Status: VALIDATED

Source sequence(s)

JAXUCZ010000004

UniProtKB/Swiss-Prot

P0C1X8

UniProtKB/TrEMBL

F1LRI7

Related

ENSRNOP00000044665.4, ENSRNOT00000043082.5

Conserved Domains (2) summary

smart00220
Location:47 → 307

S_TKc; Serine/Threonine protein kinases, catalytic domain

cd14037
Location:42 → 316

STKc_NAK_like; Catalytic domain of Numb-Associated Kinase (NAK)-like Serine/Threonine kinases

RefSeqs of Annotated Genomes: GCF_036323735.1-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCr8

Genomic

NC_086022.1 Reference GRCr8

Range

120845012..121009170

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_017592818.3 → XP_017448307.1 AP2-associated protein kinase 1 isoform X1
XM_017592822.3 → XP_017448311.1 AP2-associated protein kinase 1 isoform X10

UniProtKB/TrEMBL

A0A8I6A256

Related

ENSRNOP00000084865.1
XM_006236833.5 → XP_006236895.1 AP2-associated protein kinase 1 isoform X12

See identical proteins and their annotated locations for XP_006236895.1

UniProtKB/Swiss-Prot

P0C1X8

UniProtKB/TrEMBL

F1LRI7

Conserved Domains (2) summary

smart00220
Location:47 → 307

S_TKc; Serine/Threonine protein kinases, catalytic domain

cd14037
Location:42 → 316

STKc_NAK_like; Catalytic domain of Numb-Associated Kinase (NAK)-like Serine/Threonine kinases
XM_017592817.3 → XP_017448306.1 AP2-associated protein kinase 1 isoform X1
XM_017592820.3 → XP_017448309.1 AP2-associated protein kinase 1 isoform X4
XM_039108160.2 → XP_038964088.1 AP2-associated protein kinase 1 isoform X5

Conserved Domains (3) summary

cd14037
Location:42 → 316

STKc_NAK_like; Catalytic domain of Numb-Associated Kinase (NAK)-like Serine/Threonine kinases

pfam05109
Location:320 → 423

Herpes_BLLF1; Herpes virus major outer envelope glycoprotein (BLLF1)

pfam15282
Location:982 → 1193

BMP2K_C; BMP-2-inducible protein kinase C-terminus
XM_039108159.2 → XP_038964087.1 AP2-associated protein kinase 1 isoform X3

Conserved Domains (3) summary

cd14037
Location:42 → 316

STKc_NAK_like; Catalytic domain of Numb-Associated Kinase (NAK)-like Serine/Threonine kinases

pfam05109
Location:320 → 423

Herpes_BLLF1; Herpes virus major outer envelope glycoprotein (BLLF1)

pfam15282
Location:1058 → 1269

BMP2K_C; BMP-2-inducible protein kinase C-terminus
XM_039108161.2 → XP_038964089.1 AP2-associated protein kinase 1 isoform X7

Conserved Domains (3) summary

cd14037
Location:42 → 316

STKc_NAK_like; Catalytic domain of Numb-Associated Kinase (NAK)-like Serine/Threonine kinases

pfam05109
Location:320 → 423

Herpes_BLLF1; Herpes virus major outer envelope glycoprotein (BLLF1)

pfam15282
Location:950 → 1161

BMP2K_C; BMP-2-inducible protein kinase C-terminus
XM_017592819.3 → XP_017448308.1 AP2-associated protein kinase 1 isoform X2
XM_063286530.1 → XP_063142600.1 AP2-associated protein kinase 1 isoform X6
XM_017592821.3 → XP_017448310.1 AP2-associated protein kinase 1 isoform X8

UniProtKB/TrEMBL

A0A8I6ALS6

Related

ENSRNOP00000094963.1
XM_063286531.1 → XP_063142601.1 AP2-associated protein kinase 1 isoform X9
XM_039108162.2 → XP_038964090.1 AP2-associated protein kinase 1 isoform X11

Conserved Domains (3) summary

cd14037
Location:42 → 316

STKc_NAK_like; Catalytic domain of Numb-Associated Kinase (NAK)-like Serine/Threonine kinases

pfam05109
Location:320 → 423

Herpes_BLLF1; Herpes virus major outer envelope glycoprotein (BLLF1)

pfam15282
Location:915 → 1126

BMP2K_C; BMP-2-inducible protein kinase C-terminus
XM_063286532.1 → XP_063142602.1 AP2-associated protein kinase 1 isoform X14
XM_006236834.5 → XP_006236896.1 AP2-associated protein kinase 1 isoform X13

UniProtKB/Swiss-Prot

P0C1X8

Conserved Domains (2) summary

smart00220
Location:47 → 307

S_TKc; Serine/Threonine protein kinases, catalytic domain

cd14037
Location:42 → 316

STKc_NAK_like; Catalytic domain of Numb-Associated Kinase (NAK)-like Serine/Threonine kinases