GSTA3 glutathione S-transferase alpha 3 [Homo sapiens (human)] - Gene

Summary

Official Symbol: GSTA3provided by HGNC
Official Full Name: glutathione S-transferase alpha 3provided by HGNC
Primary source: HGNC:HGNC:4628
See related: Ensembl:ENSG00000174156 MIM:605449; AllianceGenome:HGNC:4628
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Homo sapiens
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo
Also known as: GTA3; GSTA3-3
Summary: Cytosolic and membrane-bound forms of glutathione S-transferase are encoded by two distinct supergene families. These enzymes are involved in cellular defense against toxic, carcinogenic, and pharmacologically active electrophilic compounds. At present, eight distinct classes of the soluble cytoplasmic mammalian glutathione S-transferases have been identified: alpha, kappa, mu, omega, pi, sigma, theta and zeta. This gene encodes a glutathione S-tranferase belonging to the alpha class genes that are located in a cluster mapped to chromosome 6. Genes of the alpha class are highly related and encode enzymes with glutathione peroxidase activity. However, during evolution, this alpha class gene diverged accumulating mutations in the active site that resulted in differences in substrate specificity and catalytic activity. The enzyme encoded by this gene catalyzes the double bond isomerization of precursors for progesterone and testosterone during the biosynthesis of steroid hormones. An additional transcript variant has been identified, but its full length sequence has not been determined. [provided by RefSeq, Jul 2008]
Expression: Biased expression in adrenal (RPKM 10.7), placenta (RPKM 5.5) and 2 other tissues See more
Orthologs: all
NEW: Try the new Gene table
Try the new Transcript table

Genomic context

Location:: 6p12.2

Exon count:: 9

Annotation release	Status	Assembly	Chr	Location
RS_2023_10	current	GRCh38.p14 (GCF_000001405.40)	6	NC_000006.12 (52896646..52909698, complement)
RS_2023_10	current	T2T-CHM13v2.0 (GCF_009914755.1)	6	NC_060930.1 (52736167..52749210, complement)
105.20220307	previous assembly	GRCh37.p13 (GCF_000001405.25)	6	NC_000006.11 (52761444..52774496, complement)

Chromosome 6 - NC_000006.12 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

Go to the top of the page Help

Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

Go to the top of the page Help

See details

Project title: HPA RNA-seq normal tissues
Description: RNA-seq was performed of tissue samples from 95 human individuals representing 27 different tissues in order to determine tissue-specificity of all protein-coding genes
BioProject: PRJEB4337
Publication: PMID 24309898
Analysis date: Wed Apr 4 07:08:55 2018

Bibliography

Go to the top of the page Help

GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Inhibitory effect of glutathione S-transferase A3 in the progression of cutaneous squamous cell carcinoma.
Title: Inhibitory effect of glutathione S-transferase A3 in the progression of cutaneous squamous cell carcinoma.
Certain core genes, including COL12A1, glutathione Stransferase alpha3 (GSTA3), fibrinogen alpha chain (FGA) and fibrinogen gamma chain (FGG), were the first reported to be associated with Gastric Cancer. Survival analysis suggested that these four genes, COL12A1 (P=0.002), GSTA3 (P=3.4x106), FGA (P=0.00075) and FGG (P=1.4x105), were significant poor prognostic factors of gastric cancer.
Title: Novel prognostic biomarkers of gastric cancer based on gene expression microarray: COL12A1, GSTA3, FGA and FGG.
GSTA3 expression is significantly downregulated in human masticatory mucosa during wound healing
Title: Human gingiva transcriptome during wound healing.
Density functional theory calculations used to propose a refined mechanism for the isomerization of delta(5)-androstene-3,17-dione catalyzed by GST A3-3
Title: Isomerization of Δ5-androstene-3,17-dione into Δ4-androstene-3,17-dione catalyzed by human glutathione transferase A3-3: a computational study identifies a dual role for glutathione.
analysis of how isomerization of the steroid Delta(5)-androstene-3,17-dione by the glutathione transferase A3-3
Title: The isomerization of Δ5-androstene-3,17-dione by the human glutathione transferase A3-3 proceeds via a conjugated heteroannular diene intermediate.
Data indicate that steroidogenic factor 1 (SF-1) and glutathione S-transferase A (GSTA) family genes (hGSTA1-hGSTA4) are involved in steroidogenesis.
Title: Human glutathione S-transferase A (GSTA) family genes are regulated by steroidogenic factor 1 (SF-1) and are involved in steroidogenesis.
The 3D structures of human GSTs A2-2 and A3-3 in complex with Delta(4)-androstene-3-17-dione, were solved.
Title: Structural basis for featuring of steroid isomerase activity in alpha class glutathione transferases.
Observational study of gene-disease association. (HuGE Navigator)
Title: New genetic associations detected in a host response study to hepatitis B vaccine.
Significantly contributes to the double-bond isomerization necessary for steroid hormone biosynthesis in complex systems, thereby complementing the indispensable 3beta-hydroxysteroid oxidoreductase activity of 3beta-hydroxysteroid dehydrogenase.
Title: Targeting human glutathione transferase A3-3 attenuates progesterone production in human steroidogenic cells.
Analysis of the active centers of hGSTA3-3 reveals that residues in positions 12 and 208 may contribute to its disparate isomerase activity toward Delta(5)-AD.
Title: Crystal structure of human glutathione S-transferase A3-3 and mechanistic implications for its high steroid isomerase activity.

Submit: New GeneRIF Correction See all GeneRIFs (11)

Phenotypes

Go to the top of the page Help

Find tests for this gene in the NIH Genetic Testing Registry (GTR)

Review eQTL and phenotype association data in this region using PheGenI

Variation

Go to the top of the page Help

See variants in ClinVar

See studies and variants in dbVar

See Variation Viewer (GRCh37.p13)

See Variation Viewer (GRCh38)

Interactions

Go to the top of the page Help

Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

Go to the top of the page Help

Markers

SHGC-111737 (e-PCR)
- UniSTS:168721

RH71356 (e-PCR)
- UniSTS:76642

Gene Ontology Provided by GOA

Function	Evidence Code	Pubs
enables glutathione transferase activity	IBA Inferred from Biological aspect of Ancestor more info
enables glutathione transferase activity	ISS Inferred from Sequence or Structural Similarity more info

Process	Evidence Code	Pubs
involved_in glutathione metabolic process	IBA Inferred from Biological aspect of Ancestor more info
involved_in glutathione metabolic process	ISS Inferred from Sequence or Structural Similarity more info
involved_in lipid metabolic process	IEA Inferred from Electronic Annotation more info
involved_in xenobiotic metabolic process	IBA Inferred from Biological aspect of Ancestor more info

Component	Evidence Code	Pubs
is_active_in cytosol	IBA Inferred from Biological aspect of Ancestor more info
located_in cytosol	IDA Inferred from Direct Assay more info
located_in cytosol	TAS Traceable Author Statement more info
located_in extracellular exosome	HDA more info	PubMed

General protein information

Go to the top of the page Help

Preferred Names: glutathione S-transferase A3

Names: GST class-alpha member 3; S-(hydroxyalkyl)glutathione lyase A3; glutathione S-alkyltransferase A3; glutathione S-aralkyltransferase A3; glutathione S-aryltransferase A3; glutathione S-transferase A3-3

NP_000838.3

EC 2.5.1.18

NP_001350471.1

EC 2.5.1.18

XP_006715135.1

EC 2.5.1.18

XP_011512835.1

EC 2.5.1.18

XP_047274641.1

EC 2.5.1.18

XP_054211233.1

EC 2.5.1.18

XP_054211234.1

EC 2.5.1.18

XP_054211235.1

EC 2.5.1.18

NCBI Reference Sequences (RefSeq)

Go to the top of the page Help

NEW Try the new Transcript table

RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_000847.5 → NP_000838.3 glutathione S-transferase A3 isoform 1

See identical proteins and their annotated locations for NP_000838.3

Status: REVIEWED

Description

Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1).

Source sequence(s)

AI199811, AL121969, BC020619, DT932587

Consensus CDS

CCDS4947.1

UniProtKB/Swiss-Prot

O43468, Q068V6, Q16772, Q8WWA8, Q9H415

Related

ENSP00000211122.3, ENST00000211122.4

Conserved Domains (2) summary

cd03208
Location:86 → 220

GST_C_Alpha; C-terminal, alpha helical domain of Class Alpha Glutathione S-transferases

cd03077
Location:4 → 82

GST_N_Alpha; GST_N family, Class Alpha subfamily; GSTs are cytosolic dimeric proteins involved in cellular detoxification by catalyzing the conjugation of glutathione (GSH) with a wide range of endogenous and xenobiotic alkylating agents, including carcinogens, ...
NM_001363542.2 → NP_001350471.1 glutathione S-transferase A3 isoform 2

Status: REVIEWED

Description

Transcript Variant: This variant (2) differs in the 5' UTR and coding sequence compared to variant 1. The resulting isoform (2) is shorter at the N-terminus compared to isoform 1.

Source sequence(s)

AF020919, AL121969

Consensus CDS

CCDS87412.1

UniProtKB/TrEMBL

Q5JW85

Related

ENSP00000360007.1, ENST00000370968.5

Conserved Domains (2) summary

cd03208
Location:36 → 170

GST_C_Alpha; C-terminal, alpha helical domain of Class Alpha Glutathione S-transferases

cl00388
Location:1 → 32

Thioredoxin_like; Protein Disulfide Oxidoreductases and Other Proteins with a Thioredoxin fold

RefSeqs of Annotated Genomes: GCF_000001405.40-RS_2023_10

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCh38.p14 Primary Assembly

Genomic

NC_000006.12 Reference GRCh38.p14 Primary Assembly

Range

52896646..52909698 complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_006715072.5 → XP_006715135.1 glutathione S-transferase A3 isoform X1

See identical proteins and their annotated locations for XP_006715135.1

UniProtKB/TrEMBL

Q5JW85

Conserved Domains (2) summary

cd03208
Location:36 → 170

GST_C_Alpha; C-terminal, alpha helical domain of Class Alpha Glutathione S-transferases

cl00388
Location:1 → 32

Thioredoxin_like; Protein Disulfide Oxidoreductases and Other Proteins with a Thioredoxin fold
XM_047418685.1 → XP_047274641.1 glutathione S-transferase A3 isoform X1

UniProtKB/TrEMBL

Q5JW85
XM_011514533.3 → XP_011512835.1 glutathione S-transferase A3 isoform X2

Conserved Domains (1) summary

cd03208
Location:1 → 127

GST_C_Alpha; C-terminal, alpha helical domain of Class Alpha Glutathione S-transferases

RNA

XR_926166.3 RNA Sequence
XR_926167.3 RNA Sequence

Alternate T2T-CHM13v2.0

Genomic

NC_060930.1 Alternate T2T-CHM13v2.0

Range

52736167..52749210 complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_054355259.1 → XP_054211234.1 glutathione S-transferase A3 isoform X1

UniProtKB/TrEMBL

Q5JW85
XM_054355258.1 → XP_054211233.1 glutathione S-transferase A3 isoform X1

UniProtKB/TrEMBL

Q5JW85
XM_054355260.1 → XP_054211235.1 glutathione S-transferase A3 isoform X2