Wasl WASP like actin nucleation promoting factor [Rattus norvegicus (Norway rat)] - Gene

Summary

Official Symbol: Waslprovided by RGD
Official Full Name: WASP like actin nucleation promoting factorprovided by RGD
Primary source: RGD:735144
See related: Ensembl:ENSRNOG00000006975 AllianceGenome:RGD:735144
Gene type: protein coding
RefSeq status: PROVISIONAL
Organism: Rattus norvegicus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Rattus
Also known as: TRS4; N-WASP
Summary: Enables cytoskeletal protein binding activity and identical protein binding activity. Involved in several processes, including plasma membrane tubulation; positive regulation of cellular component organization; and postsynaptic actin cytoskeleton organization. Located in Golgi membrane; cell leading edge; and cytoplasmic vesicle. Is active in glutamatergic synapse and postsynapse. Orthologous to human WASL (WASP like actin nucleation promoting factor). [provided by Alliance of Genome Resources, Apr 2022]
Expression: Biased expression in Kidney (RPKM 186.2), Adrenal (RPKM 155.7) and 9 other tissues See more
Orthologs: human mouse all
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Genomic context

Location:: 4q22

Exon count:: 12

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCr8 (GCF_036323735.1)	4	NC_086022.1 (54049081..54097653, complement)
RS_2023_06	previous assembly	mRatBN7.2 (GCF_015227675.2)	4	NC_051339.1 (53083536..53132061, complement)
106	previous assembly	Rnor_6.0 (GCF_000001895.5)	4	NC_005103.4 (51677722..51726212, complement)

Chromosome 4 - NC_086022.1 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: A rat RNA-Seq transcriptomic BodyMap across 11 organs and 4 developmental stages
Description: 320 RNA samples isolated from 11 organs (adrenal gland, brain, heart, kidney, liver, lung, muscle, spleen, thymus, and testes or uterus) from both sexes of Fischer 344 rats across four developmental stages (2-, 6-, 21-, and 104-weeks-old)
BioProject: PRJNA238328
Publication: PMID 24510058
Analysis date: Mon Jun 6 17:44:12 2016

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Phosphorylation of PACSIN2 at S313 Regulates Podocyte Architecture in Coordination with N-WASP.
Title: Phosphorylation of PACSIN2 at S313 Regulates Podocyte Architecture in Coordination with N-WASP.
alphaII-spectrin controls calcium-regulated exocytosis in neuroendocrine chromaffin cells through neuronal Wiskott-Aldrich Syndrome protein interaction.
Title: αII-spectrin controls calcium-regulated exocytosis in neuroendocrine chromaffin cells through neuronal Wiskott-Aldrich Syndrome protein interaction.
Nck was the most potent activator of N-WASP-driven actin assembly.
Title: Allosteric N-WASP activation by an inter-SH3 domain linker in Nck.
biochemical reconstitution on supported lipid bilayers of protein clusters containing the adhesion receptor Nephrin and its cytoplasmic partners, Nck and N-WASP, is reported.
Title: Phase transitions of multivalent proteins can promote clustering of membrane receptors.
The Arp2/3 regulators PICK1, and N-WASP and WAVE2 function antagonistically to control the complexity of astrocyte branched morphology, and this mechanism underlies the morphological changes seen in astrocytes during their response to pathological insult.
Title: The antagonistic modulation of Arp2/3 activity by N-WASP, WAVE2 and PICK1 defines dynamic changes in astrocyte morphology.
N-WASP/IRSp53 interaction helps to regulate filopodial formation and cell adhesion in mammary epithelial cells.
Title: The type III TGFβ receptor regulates filopodia formation via a Cdc42-mediated IRSp53-N-WASP interaction in epithelial cells.
Dyrk1A negatively regulates actin filament assembly by phosphorylating N-WASP, which ultimately promotes the intramolecular interaction of its GTPase-binding and verprolin, cofilin and acidic domains
Title: Dyrk1A negatively regulates the actin cytoskeleton through threonine phosphorylation of N-WASP.
tumor cells in vivo that have a decreased activity of N-WASP also have a reduced ability to form invadopodia, migrate, invade, intravasate and disseminate to lung compared with tumor cells with parental N-WASP levels
Title: N-WASP-mediated invadopodium formation is involved in intravasation and lung metastasis of mammary tumors.
A functional interplay of a trimeric complex that transforms incoming signals via SK3-channel activation into the local rearrangement of the cytoskeleton in early steps of neuronal differentiation involving nWASP and Abi-1 actin binding proteins.
Title: An SK3 channel/nWASP/Abi-1 complex is involved in early neurogenesis.
amphiphysin interaction with N-WASP regulates actin assembly
Title: Dynamic interaction of amphiphysin with N-WASP regulates actin assembly.

Submit: New GeneRIF Correction See all GeneRIFs (25)

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

AI231276 (e-PCR)
- UniSTS:260753

RH128038 (e-PCR)
- UniSTS:211346

Gene Ontology Provided by RGD

Function	Evidence Code	Pubs
enables actin binding	IEA Inferred from Electronic Annotation more info
enables cytoskeletal protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables identical protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed

Process	Evidence Code	Pubs
acts_upstream_of_or_within actin cytoskeleton organization	ISO Inferred from Sequence Orthology more info
involved_in actin filament organization	IEA Inferred from Electronic Annotation more info
involved_in actin filament polymerization	IEA Inferred from Electronic Annotation more info
involved_in actin filament polymerization	ISO Inferred from Sequence Orthology more info
involved_in actin filament polymerization	ISS Inferred from Sequence or Structural Similarity more info
involved_in cell division	IEA Inferred from Electronic Annotation more info
NOT acts_upstream_of_or_within cell projection assembly	ISO Inferred from Sequence Orthology more info
involved_in dendritic spine morphogenesis	IEA Inferred from Electronic Annotation more info
involved_in dendritic spine morphogenesis	ISO Inferred from Sequence Orthology more info
involved_in dendritic spine morphogenesis	ISS Inferred from Sequence or Structural Similarity more info
involved_in membrane invagination	IDA Inferred from Direct Assay more info	PubMed
involved_in negative regulation of lymphocyte migration	IEA Inferred from Electronic Annotation more info
involved_in negative regulation of lymphocyte migration	ISO Inferred from Sequence Orthology more info
involved_in negative regulation of membrane tubulation	IEA Inferred from Electronic Annotation more info
involved_in negative regulation of membrane tubulation	ISO Inferred from Sequence Orthology more info
involved_in plasma membrane tubulation	IDA Inferred from Direct Assay more info	PubMed
involved_in positive regulation of chemotaxis	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of clathrin-dependent endocytosis	IDA Inferred from Direct Assay more info	PubMed
involved_in positive regulation of filopodium assembly	IDA Inferred from Direct Assay more info	PubMed
involved_in positive regulation of transcription by RNA polymerase II	IEA Inferred from Electronic Annotation more info
involved_in positive regulation of transcription by RNA polymerase II	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of transcription by RNA polymerase II	ISS Inferred from Sequence or Structural Similarity more info
involved_in postsynapse organization	EXP Inferred from Experiment more info	PubMed
involved_in postsynapse organization	IDA Inferred from Direct Assay more info	PubMed
involved_in postsynapse organization	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in postsynaptic actin cytoskeleton organization	EXP Inferred from Experiment more info	PubMed
involved_in postsynaptic actin cytoskeleton organization	IDA Inferred from Direct Assay more info	PubMed
involved_in postsynaptic actin cytoskeleton organization	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in protein-containing complex localization	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within protein-containing complex localization	ISO Inferred from Sequence Orthology more info
involved_in regulation of cell projection assembly	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of postsynapse organization	IEA Inferred from Electronic Annotation more info
involved_in regulation of postsynapse organization	ISO Inferred from Sequence Orthology more info
involved_in regulation of protein localization	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within regulation of protein localization	ISO Inferred from Sequence Orthology more info
involved_in response to bacterium	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within response to bacterium	ISO Inferred from Sequence Orthology more info
involved_in spindle localization	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within spindle localization	ISO Inferred from Sequence Orthology more info
involved_in vesicle budding from membrane	IDA Inferred from Direct Assay more info	PubMed
involved_in vesicle organization	IDA Inferred from Direct Assay more info	PubMed
involved_in vesicle transport along actin filament	IDA Inferred from Direct Assay more info	PubMed

Component	Evidence Code	Pubs
located_in Golgi membrane	IDA Inferred from Direct Assay more info	PubMed
located_in actin cap	IEA Inferred from Electronic Annotation more info
located_in actin cap	ISO Inferred from Sequence Orthology more info
located_in cell leading edge	IDA Inferred from Direct Assay more info	PubMed
located_in cytoplasm	IEA Inferred from Electronic Annotation more info
located_in cytoplasmic vesicle	IDA Inferred from Direct Assay more info	PubMed
located_in cytoskeleton	IEA Inferred from Electronic Annotation more info
located_in cytosol	IEA Inferred from Electronic Annotation more info
located_in cytosol	ISO Inferred from Sequence Orthology more info
located_in cytosol	TAS Traceable Author Statement more info
is_active_in glutamatergic synapse	EXP Inferred from Experiment more info	PubMed
is_active_in glutamatergic synapse	IDA Inferred from Direct Assay more info	PubMed
located_in glutamatergic synapse	IEA Inferred from Electronic Annotation more info
is_active_in glutamatergic synapse	IMP Inferred from Mutant Phenotype more info	PubMed
is_active_in glutamatergic synapse	ISO Inferred from Sequence Orthology more info
located_in lamellipodium	IEA Inferred from Electronic Annotation more info
located_in lamellipodium	ISO Inferred from Sequence Orthology more info
located_in nucleus	IEA Inferred from Electronic Annotation more info
located_in nucleus	ISO Inferred from Sequence Orthology more info
located_in nucleus	ISS Inferred from Sequence or Structural Similarity more info
is_active_in postsynapse	IDA Inferred from Direct Assay more info	PubMed
is_active_in postsynapse	IMP Inferred from Mutant Phenotype more info	PubMed

General protein information

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Preferred Names: actin nucleation-promoting factor WASL

Names: Wiskott-Aldrich syndrome-like; neural Wiskott-Aldrich syndrome protein

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_001110365.1 → NP_001103835.1 actin nucleation-promoting factor WASL

See identical proteins and their annotated locations for NP_001103835.1

Status: PROVISIONAL

Source sequence(s)

CH473959

UniProtKB/Swiss-Prot

O08816

UniProtKB/TrEMBL

A6IE88, F1LSG0

Related

ENSRNOP00000009261.6, ENSRNOT00000009261.7

Conserved Domains (4) summary

pfam00568
Location:28 → 135

WH1; WH1 domain

pfam00786
Location:199 → 256

PBD; P21-Rho-binding domain

cd22074
Location:398 → 424

WH2_N-WASP_r1; first tandem Wiskott Aldrich syndrome homology region 2 (WH2 motif) repeat found in human Neural Wiskott-Aldrich syndrome protein (N-WASP) and related domains

cd22075
Location:425 → 449

WH2_hN-WASP_r2_like; second tandem Wiskott Aldrich syndrome homology region 2 (WH2 motif) repeat found in human Neural Wiskott-Aldrich syndrome protein (N-WASP) and related domains

RefSeqs of Annotated Genomes: GCF_036323735.1-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCr8

Genomic

NC_086022.1 Reference GRCr8

Range

54049081..54097653 complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_039108349.2 → XP_038964277.1 actin nucleation-promoting factor WASL isoform X1

UniProtKB/Swiss-Prot

O08816

Conserved Domains (4) summary

pfam00568
Location:28 → 135

WH1; WH1 domain

pfam00786
Location:199 → 256

PBD; P21-Rho-binding domain

cd22074
Location:404 → 430

WH2_N-WASP_r1; first tandem Wiskott Aldrich syndrome homology region 2 (WH2 motif) repeat found in human Neural Wiskott-Aldrich syndrome protein (N-WASP) and related domains

cd22075
Location:431 → 455

WH2_hN-WASP_r2_like; second tandem Wiskott Aldrich syndrome homology region 2 (WH2 motif) repeat found in human Neural Wiskott-Aldrich syndrome protein (N-WASP) and related domains