Dna2 DNA replication helicase/nuclease 2 [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: Dna2provided by MGI
Official Full Name: DNA replication helicase/nuclease 2provided by MGI
Primary source: MGI:MGI:2443732
See related: Ensembl:ENSMUSG00000036875 AllianceGenome:MGI:2443732
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as: Dna2l; E130315B21Rik
Summary: Enables 5'-flap endonuclease activity. Involved in mitotic telomere maintenance via semi-conservative replication and nucleic acid phosphodiester bond hydrolysis. Located in chromosome, telomeric region. Human ortholog(s) of this gene implicated in Seckel syndrome 8; autosomal dominant progressive external ophthalmoplegia with mitochondrial DNA deletions 6; isolated growth hormone deficiency type IA; and mitochondrial myopathy. Orthologous to human DNA2 (DNA replication helicase/nuclease 2). [provided by Alliance of Genome Resources, Apr 2022]
Expression: Broad expression in liver E14 (RPKM 14.4), liver E14.5 (RPKM 13.2) and 18 other tissues See more
Orthologs: human all
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Genomic context

Location:: 10 B4; 10 32.51 cM

Exon count:: 21

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCm39 (GCF_000001635.27)	10	NC_000076.7 (62782762..62809967)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	10	NC_000076.6 (62946983..62974188)

Chromosome 10 - NC_000076.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Genomic Sequence:

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Expression

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See details

Project title: Mouse ENCODE transcriptome data
Description: RNA profiling data sets generated by the Mouse ENCODE project.
BioProject: PRJNA66167
Publication: PMID 25409824
Analysis date: n/a

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

The authors establish that a second Dna2-Rpa interaction is mutually exclusive with Rpa-DNA interactions and mediates the displacement of Rpa from ssDNA.
Title: Dna2 nuclease-helicase structure, mechanism and regulation by Rpa.
DNA2 helicase cleaves G-quadruplex DNA and is required for telomere integrity.DNA2 reduces replication stress at telomeres, thereby preserving genome stability and suppressing cancer development.
Title: Mammalian DNA2 helicase/nuclease cleaves G-quadruplex DNA and is required for telomere integrity.
Characterization of the endonuclease and ATP-dependent flap endo/exonuclease of Dna2.
Title: Characterization of the endonuclease and ATP-dependent flap endo/exonuclease of Dna2.

Submit: New GeneRIF Correction

Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Endonuclease-mediated (1)
Targeted (1) 1 citation

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

RH135983 (e-PCR)
- UniSTS:209979

C88009 (e-PCR)
- UniSTS:208548

Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables 4 iron, 4 sulfur cluster binding	IEA Inferred from Electronic Annotation more info
enables 5'-3' DNA helicase activity	ISO Inferred from Sequence Orthology more info
enables 5'-flap endonuclease activity	IBA Inferred from Biological aspect of Ancestor more info
enables 5'-flap endonuclease activity	IDA Inferred from Direct Assay more info	PubMed
enables 5'-flap endonuclease activity	ISO Inferred from Sequence Orthology more info
enables ATP binding	IEA Inferred from Electronic Annotation more info
enables DNA binding	ISO Inferred from Sequence Orthology more info
enables RNA binding	IBA Inferred from Biological aspect of Ancestor more info
enables catalytic activity	IEA Inferred from Electronic Annotation more info
enables endonuclease activity	IEA Inferred from Electronic Annotation more info
enables helicase activity	IEA Inferred from Electronic Annotation more info
enables hydrolase activity	IEA Inferred from Electronic Annotation more info
enables iron-sulfur cluster binding	IEA Inferred from Electronic Annotation more info
enables metal ion binding	IEA Inferred from Electronic Annotation more info
enables nuclease activity	ISO Inferred from Sequence Orthology more info
enables nucleotide binding	IEA Inferred from Electronic Annotation more info
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables single-stranded DNA helicase activity	ISO Inferred from Sequence Orthology more info
enables site-specific endodeoxyribonuclease activity, specific for altered base	ISO Inferred from Sequence Orthology more info

Process	Evidence Code	Pubs
acts_upstream_of_or_within DNA damage response	IEA Inferred from Electronic Annotation more info
involved_in DNA double-strand break processing	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within DNA repair	IEA Inferred from Electronic Annotation more info
involved_in DNA replication	ISO Inferred from Sequence Orthology more info
involved_in DNA replication checkpoint signaling	ISO Inferred from Sequence Orthology more info
involved_in DNA replication, Okazaki fragment processing	ISO Inferred from Sequence Orthology more info
involved_in DNA replication, removal of RNA primer	ISO Inferred from Sequence Orthology more info
involved_in base-excision repair	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within metabolic process	IEA Inferred from Electronic Annotation more info
involved_in mitochondrial DNA repair	ISO Inferred from Sequence Orthology more info
involved_in mitochondrial DNA replication	ISO Inferred from Sequence Orthology more info
involved_in mitotic telomere maintenance via semi-conservative replication	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in nucleic acid metabolic process	IDA Inferred from Direct Assay more info	PubMed
involved_in positive regulation of DNA replication	ISO Inferred from Sequence Orthology more info
involved_in replication fork reversal	IBA Inferred from Biological aspect of Ancestor more info
involved_in telomere maintenance	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
located_in chromosome, telomeric region	IDA Inferred from Direct Assay more info	PubMed
is_active_in cytoplasm	IBA Inferred from Biological aspect of Ancestor more info
part_of gamma DNA polymerase complex	ISO Inferred from Sequence Orthology more info
located_in mitochondrial nucleoid	ISO Inferred from Sequence Orthology more info
located_in mitochondrion	ISO Inferred from Sequence Orthology more info
located_in nucleoplasm	ISO Inferred from Sequence Orthology more info
located_in nucleus	ISO Inferred from Sequence Orthology more info

General protein information

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Preferred Names: DNA replication ATP-dependent helicase/nuclease DNA2

Names: DNA replication ATP-dependent helicase-like homolog; DNA replication helicase 2 homolog; DNA2-like helicase

NP_796346.2

EC 3.6.4.12

XP_036011717.1

EC 3.6.4.12

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_177372.3 → NP_796346.2 DNA replication ATP-dependent helicase/nuclease DNA2

See identical proteins and their annotated locations for NP_796346.2

Status: VALIDATED

Source sequence(s)

AK028381, AK053856, BM202919, BY768981, CO433698

Consensus CDS

CCDS35923.1

UniProtKB/Swiss-Prot

Q14BM9, Q6ZQJ5, Q8BSZ0, Q8R3J3

Related

ENSMUSP00000115750.2, ENSMUST00000131422.8

Conserved Domains (5) summary

TIGR00376
Location:472 → 1034

TIGR00376; DNA helicase, putative

pfam08696
Location:72 → 283

Dna2; DNA replication factor Dna2

pfam13087
Location:806 → 1020

AAA_12; AAA domain

cl00641
Location:256 → 398

Cas4_I-A_I-B_I-C_I-D_II-B; CRISPR/Cas system-associated protein Cas4

cl21455
Location:626 → 830

P-loop_NTPase; P-loop containing Nucleoside Triphosphate Hydrolases

RNA

NR_152862.1 RNA Sequence

Status: VALIDATED

Source sequence(s)

AA407737, AK028381, AK053856, BY768981, CO433698

RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCm39 C57BL/6J

Genomic

NC_000076.7 Reference GRCm39 C57BL/6J

Range

62782762..62809967

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_036155824.1 → XP_036011717.1 DNA replication ATP-dependent helicase/nuclease DNA2 isoform X1

Related

ENSMUSP00000090119.6, ENSMUST00000092462.12

Conserved Domains (3) summary

TIGR00376
Location:472 → 934

TIGR00376; DNA helicase, putative

cd18041
Location:626 → 829

DEXXQc_DNA2; DEXXQ-box helicase domain of DNA2

cd22318
Location:127 → 366

DNA2_N-like; Nuclease domain of the nuclease/helicase DNA2 and related nucleases