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    Marchf5 membrane associated ring-CH-type finger 5 [ Mus musculus (house mouse) ]

    Gene ID: 69104, updated on 12-May-2024

    Summary

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    Official Symbol
    Marchf5provided by MGI
    Official Full Name
    membrane associated ring-CH-type finger 5provided by MGI
    Primary source
    MGI:MGI:1915207
    See related
    Ensembl:ENSMUSG00000023307 AllianceGenome:MGI:1915207
    Gene type
    protein coding
    RefSeq status
    VALIDATED
    Organism
    Mus musculus
    Lineage
    Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
    Also known as
    MITOL; March5; Marchv; Rnf153; MARCH-V; 1810015H18Rik; 2310008I22Rik; 2700055A20Rik; 5730499H23Rik; E130202O05Rik
    Summary
    Predicted to enable GTPase binding activity and ubiquitin protein ligase activity. Predicted to be involved in several processes, including negative regulation of cell aging; positive regulation of mitochondrial fission; and protein ubiquitination. Predicted to be located in endoplasmic reticulum membrane and mitochondrial outer membrane. Orthologous to human MARCHF5 (membrane associated ring-CH-type finger 5). [provided by Alliance of Genome Resources, Apr 2022]
    Expression
    Ubiquitous expression in liver E14 (RPKM 15.2), CNS E14 (RPKM 13.7) and 28 other tissues See more
    Orthologs
    human all
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    Genomic context

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    See Marchf5 in Genome Data Viewer
    Location:
    19 C2; 19 32.2 cM
    Exon count:
    6
    Annotation release Status Assembly Chr Location
    RS_2024_02 current GRCm39 (GCF_000001635.27) 19 NC_000085.7 (37184939..37201856)
    108.20200622 previous assembly GRCm38.p6 (GCF_000001635.26) 19 NC_000085.6 (37207540..37224457)

    Chromosome 19 - NC_000085.7Genomic Context describing neighboring genes Neighboring gene STARR-seq mESC enhancer starr_46041 Neighboring gene B-TFIID TATA-box binding protein associated factor 1 Neighboring gene cytoplasmic polyadenylation element binding protein 3 Neighboring gene RIKEN cDNA A330032B11 gene Neighboring gene predicted gene, 25268 Neighboring gene galactose-3-O-sulfotransferase 1 pseudogene

    Genomic regions, transcripts, and products

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    Go to nucleotide: Graphics FASTA GenBank

    Expression

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    • Project title: Mouse ENCODE transcriptome data
    • Description: RNA profiling data sets generated by the Mouse ENCODE project.
    • BioProject: PRJNA66167
    • Publication: PMID 25409824
    • Analysis date: n/a

    Bibliography

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    Related articles in PubMed

    1. Mitochondrial E3 ubiquitin ligase MARCH5 is required for mouse oocyte meiotic maturation†. Zhou Q, et al. Biol Reprod, 2023 Mar 13. PMID 36503987
    2. Identification of highest neurotoxic amyloid-β plaque type showing reduced contact with astrocytes. Mitsubori M, et al. Biochem Biophys Res Commun, 2021 Apr 16. PMID 33667711
    3. Mitochondrial ubiquitin ligase alleviates Alzheimer's disease pathology via blocking the toxic amyloid-β oligomer generation. Takeda K, et al. Commun Biol, 2021 Feb 12. PMID 33580194, Free PMC Article
    4. Mitochondrial E3 ligase March5 maintains stemness of mouse ES cells via suppression of ERK signalling. Gu H, et al. Nat Commun, 2015 Jun 2. PMID 26033541, Free PMC Article
    5. MITOL dysfunction causes dwarfism with anterior pituitary hypoplasia. Matsuno K, et al. J Biochem, 2020 Sep 1. PMID 32302394

    See all (35) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. PD-1 signaling negatively regulates the common cytokine receptor gamma chain via MARCH5-mediated ubiquitination and degradation to suppress anti-tumor immunity.
      Title: PD-1 signaling negatively regulates the common cytokine receptor γ chain via MARCH5-mediated ubiquitination and degradation to suppress anti-tumor immunity.
    2. Irisin improves diabetic cardiomyopathy-induced cardiac remodeling by regulating GSDMD-mediated pyroptosis through MITOL/STING signaling.
      Title: Irisin improves diabetic cardiomyopathy-induced cardiac remodeling by regulating GSDMD-mediated pyroptosis through MITOL/STING signaling.
    3. MARCH5 promotes STING pathway activation by suppressing polymer formation of oxidized STING.
      Title: MARCH5 promotes STING pathway activation by suppressing polymer formation of oxidized STING.
    4. Mitochondrial E3 ubiquitin ligase MARCH5 is required for mouse oocyte meiotic maturationdagger.
      Title: Mitochondrial E3 ubiquitin ligase MARCH5 is required for mouse oocyte meiotic maturation†.
    5. Mitochondrial ubiquitin ligase alleviates Alzheimer's disease pathology via blocking the toxic amyloid-beta oligomer generation.
      Title: Mitochondrial ubiquitin ligase alleviates Alzheimer's disease pathology via blocking the toxic amyloid-β oligomer generation.
    6. A potent protective effect of baicalein on liver injury by regulating mitochondria-related apoptosis.
      Title: A potent protective effect of baicalein on liver injury by regulating mitochondria-related apoptosis.
    7. MITOL dysfunction causes dwarfism with anterior pituitary hypoplasia.
      Title: MITOL dysfunction causes dwarfism with anterior pituitary hypoplasia.
    8. Results showed that mitochondrial abnormalities in MITOL KO brain induced mild behavioral changes. MITOL KO brain also displayed enhanced oxidative stress, which may result from increased electron leakage from mitochondria. The reduced ER-mitochondria tethering and excessive mitochondrial fission may trigger neuroinflammation through oxidative stress.
      Title: MITOL deletion in the brain impairs mitochondrial structure and ER tethering leading to oxidative stress.
    9. MARCH5 is regulated by peroxisome proliferator-activated receptor-gamma (PPARgamma) during adipogenesis and is correlated with fat mass across a panel of genetically diverse mouse strains, in ob/ob mice, and in humans. Furthermore, manipulation of MARCH5 expression in vitro and in vivo alters mitochondrial function, affects cellular metabolism, and leads to differential regulation of several metabolic genes
      Title: The E3 ligase MARCH5 is a PPARγ target gene that regulates mitochondria and metabolism in adipocytes.
    10. These findings suggest that MITOL plays a protective role against apoptosis in cardiomyocytes.
      Title: The mitochondrial ubiquitin ligase plays an anti-apoptotic role in cardiomyocytes by regulating mitochondrial fission.
    Submit: New GeneRIF Correction See all GeneRIFs (15)

    Variation

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    Alleles

    Alleles of this type are documented at Mouse Genome Informatics  (MGI)
    • Endonuclease-mediated (4) 
    • Targeted (3)  1 citation

    Interactions

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    Products Interactant Other Gene Complex Source Pubs Description

    General gene information

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    Markers

    Homology

    Clone Names

    • MGC130121, MGC130122

    Gene Ontology Provided by MGI

    Function Evidence Code Pubs
    enables GTPase binding ISO
    Inferred from Sequence Orthology
    more info
    		  
    enables metal ion binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables transferase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables ubiquitin protein ligase activity IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    enables ubiquitin protein ligase activity ISO
    Inferred from Sequence Orthology
    more info
    		  
    enables zinc ion binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    Process Evidence Code Pubs
    involved_in positive regulation of mitochondrial fission ISO
    Inferred from Sequence Orthology
    more info
    		  
    involved_in protein autoubiquitination ISO
    Inferred from Sequence Orthology
    more info
    		  
    involved_in protein localization to mitochondrion ISO
    Inferred from Sequence Orthology
    more info
    		  
    involved_in protein polyubiquitination IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    involved_in protein polyubiquitination ISO
    Inferred from Sequence Orthology
    more info
    		  
    involved_in regulation of mitochondrial fission IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    involved_in regulation of mitochondrial fission ISO
    Inferred from Sequence Orthology
    more info
    		  
    Component Evidence Code Pubs
    located_in endoplasmic reticulum ISO
    Inferred from Sequence Orthology
    more info
    		  
    located_in endoplasmic reticulum membrane ISO
    Inferred from Sequence Orthology
    more info
    		  
    located_in membrane IEA
    Inferred from Electronic Annotation
    more info
    		  
    is_active_in mitochondrial outer membrane IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    located_in mitochondrial outer membrane ISO
    Inferred from Sequence Orthology
    more info
    		  
    located_in mitochondrion ISO
    Inferred from Sequence Orthology
    more info
    		  

    General protein information

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    Preferred Names
    E3 ubiquitin-protein ligase MARCHF5
    Names
    E3 ubiquitin-protein ligase MARCH5
    RING-type E3 ubiquitin transferase MARCH5
    RING-type E3 ubiquitin transferase MARCHF5
    membrane-associated RING finger protein 5
    membrane-associated RING-CH protein V
    membrane-associated ring finger (C3HC4) 5
    ring finger protein 153
    NP_001157808.1
    NP_001157809.1
    NP_001365726.1
    NP_001365727.1
    NP_081590.3

    NCBI Reference Sequences (RefSeq)

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    RefSeqs maintained independently of Annotated Genomes

    These reference sequences exist independently of genome builds. Explain

    These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

    mRNA and Protein(s)

    1. NM_001164336.1 → NP_001157808.1  E3 ubiquitin-protein ligase MARCHF5 isoform 2

      See identical proteins and their annotated locations for NP_001157808.1

      Status: VALIDATED

      Description
      Transcript Variant: This variant (2) differs in the 3' coding region and 3' UTR, compared to variant 1. The resulting isoform (2) has a distinct C-terminus and is shorter than isoform 1.
      Source sequence(s)
      AK007511, AK009364, BY070559, CX733941
      Consensus CDS
      CCDS50427.1
      UniProtKB/TrEMBL
      A0A0R4J1H0
      Related
      ENSMUSP00000108010.2, ENSMUST00000112391.8
      Conserved Domains (1) summary
      cd16701
      Location:12 → 72
      RING_CH-C4HC3_MARCH5; RING-CH finger, H2 subclass (C4HC3-type), found in membrane-associated RING-CH5 (MARCH5)

    2. NM_001164337.1 → NP_001157809.1  E3 ubiquitin-protein ligase MARCHF5 isoform 3

      See identical proteins and their annotated locations for NP_001157809.1

      Status: VALIDATED

      Description
      Transcript Variant: This variant (3) differs in the 3' coding region and 3' UTR, compared to variant 1. The resulting isoform (3) has a distinct C-terminus and is shorter than isoform 1.
      Source sequence(s)
      AC161238, AK007511, AK009364, AK021403, BY070559
      Conserved Domains (1) summary
      smart00744
      Location:14 → 69
      RINGv; The RING-variant domain is a C4HC3 zinc-finger like motif found in a number of cellular and viral proteins

    3. NM_001378797.1 → NP_001365726.1  E3 ubiquitin-protein ligase MARCHF5 isoform 4

      Status: VALIDATED

      Source sequence(s)
      AC161238
      Conserved Domains (1) summary
      cd16701
      Location:12 → 72
      RING_CH-C4HC3_MARCH5; RING-CH finger, H2 subclass (C4HC3-type), found in membrane-associated RING-CH5 (MARCH5)

    4. NM_001378798.1 → NP_001365727.1  E3 ubiquitin-protein ligase MARCHF5 isoform 5

      Status: VALIDATED

      Source sequence(s)
      AC161238

    5. NM_027314.3 → NP_081590.3  E3 ubiquitin-protein ligase MARCHF5 isoform 1

      See identical proteins and their annotated locations for NP_081590.3

      Status: VALIDATED

      Description
      Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1).
      Source sequence(s)
      AC161238, AK003478, AK009364, AK017741, BC132455, BY070559
      Consensus CDS
      CCDS29776.1
      UniProtKB/Swiss-Prot
      Q3KNM2, Q3KNM3, Q6P230, Q9CPS3, Q9CTI6
      UniProtKB/TrEMBL
      A2RTC8
      Related
      ENSMUSP00000024078.8, ENSMUST00000024078.15
      Conserved Domains (1) summary
      cd16701
      Location:12 → 72
      RING_CH-C4HC3_MARCH5; RING-CH finger, H2 subclass (C4HC3-type), found in membrane-associated RING-CH5 (MARCH5)

    RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference GRCm39 C57BL/6J

    Genomic

    1. NC_000085.7 Reference GRCm39 C57BL/6J

      Range
      37184939..37201856
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)
    Nucleotide Protein Strain
    Heading Accession and Version
    Protein Accession Links
    GenPept Link UniProtKB Link
    Q3KNM2.1 GenPept UniProtKB/Swiss-Prot:Q3KNM2

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