Myo15a myosin XVA [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: Myo15aprovided by MGI
Official Full Name: myosin XVAprovided by MGI
Primary source: MGI:MGI:1261811
See related: Ensembl:ENSMUSG00000042678 AllianceGenome:MGI:1261811
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as: sh2; sh-2; Myo15
Summary: Predicted to enable actin filament binding activity and microfilament motor activity. Acts upstream of or within inner ear morphogenesis; locomotory behavior; and sensory perception of sound. Located in stereocilium. Is expressed in several structures, including heart; inner ear; liver; metanephros; and skeletal muscle. Used to study autosomal recessive nonsyndromic deafness 3. Human ortholog(s) of this gene implicated in autosomal recessive nonsyndromic deafness 3 and sensorineural hearing loss. Orthologous to human MYO15A (myosin XVA). [provided by Alliance of Genome Resources, Apr 2022]
Expression: Biased expression in testis adult (RPKM 2.4), cerebellum adult (RPKM 0.5) and 4 other tissues See more
Orthologs: human all
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Genomic context

Location:: 11 B2; 11 37.81 cM

Exon count:: 67

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCm39 (GCF_000001635.27)	11	NC_000077.7 (60360165..60419195)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	11	NC_000077.6 (60469339..60528369)

Chromosome 11 - NC_000077.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: Mouse ENCODE transcriptome data
Description: RNA profiling data sets generated by the Mouse ENCODE project.
BioProject: PRJNA66167
Publication: PMID 25409824
Analysis date: n/a

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

The ATPase mechanism of myosin 15, the molecular motor mutated in DFNB3 human deafness.
Title: The ATPase mechanism of myosin 15, the molecular motor mutated in DFNB3 human deafness.
Myosin-XVa Controls Both Staircase Architecture and Diameter Gradation of Stereocilia Rows in the Auditory Hair Cell Bundles.
Title: Myosin-XVa Controls Both Staircase Architecture and Diameter Gradation of Stereocilia Rows in the Auditory Hair Cell Bundles.
Gpsm2 defines an approximately 200 nm nanodomain at the tips of stereocilia and this localization requires the presence of Galphai3, myosin 15 and whirlin.
Title: Defective Gpsm2/Gα(i3) signalling disrupts stereocilia development and growth cone actin dynamics in Chudley-McCullough syndrome.
Myosin 15 isoforms can navigate between functionally distinct classes of stereocilia, and are independently required to assemble and then maintain the intricate hair bundle architecture.
Title: The 133-kDa N-terminal domain enables myosin 15 to maintain mechanotransducing stereocilia and is essential for hearing.
myo15-subfragment 1 is a barbed-end-directed motor that moves actin filaments in a gliding assay using a power stroke of 7.9 nm. Myo15-S1 was rate limited by transit through strongly actin-bound states, similar to other processive myosin motors.
Title: Chaperone-enhanced purification of unconventional myosin 15, a molecular motor specialized for stereocilia protein trafficking.
Genetic association between myoXV and deafness in a murine model.
Title: Spontaneous mutant ICR kuru2 might be another shaker-2 deaf mouse.
It was concluded that MyoXVa, whirlin, and Eps8 are integral components of the stereocilia tip complex, where Eps8 is a central actin-regulatory element for elongation of the stereocilia actin core.
Title: Regulation of stereocilia length by myosin XVa and whirlin depends on the actin-regulatory protein Eps8.
The Ca(2+) sensitivity of the mechanotransduction channels and the fast adaptation require a structural environment that is dependent on myosin-XVa and is disrupted in Myo15(sh2/sh2) inner hair cells, but not in Myo15(sh2/sh2) outer hair cells.
Title: Fast adaptation and Ca2+ sensitivity of the mechanotransducer require myosin-XVa in inner but not outer cochlear hair cells.
The morphology of cochlear hair cell stereocilia in double mutants reflects a dominance of the more severe Myo15 (sh2/sh2) phenotype over the Whrn (wi/wi) phenotype.
Title: Whirler mutant hair cells have less severe pathology than shaker 2 or double mutants.
This comparison revealed that the absence of functional myosin-XVa does not disrupt adaptation of the mechanotransduction current during sustained bundle deflection.
Title: Auditory mechanotransduction in the absence of functional myosin-XVa.

Submit: New GeneRIF Correction See all GeneRIFs (12)

Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Chemically induced (ENU) (4) 1 citation
Endonuclease-mediated (3)
Radiation induced (1)
Spontaneous (2)
Targeted (3) 1 citation

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

AI255295 (e-PCR)
- UniSTS:160100

Myo15 (e-PCR), detects polymorphism
- UniSTS:532113

RH140016 (e-PCR)
- UniSTS:222098

RH124412 (e-PCR)
- UniSTS:161234

D11Mit26 (e-PCR), detects polymorphism
- UniSTS:126461

Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables ATP binding	IEA Inferred from Electronic Annotation more info
enables actin binding	IEA Inferred from Electronic Annotation more info
enables actin filament binding	IBA Inferred from Biological aspect of Ancestor more info
enables cytoskeletal motor activity	IEA Inferred from Electronic Annotation more info
enables microfilament motor activity	IBA Inferred from Biological aspect of Ancestor more info
enables nucleotide binding	IEA Inferred from Electronic Annotation more info
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed

Process	Evidence Code	Pubs
involved_in actin filament organization	IBA Inferred from Biological aspect of Ancestor more info
involved_in endocytosis	IBA Inferred from Biological aspect of Ancestor more info
acts_upstream_of_or_within inner ear morphogenesis	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within locomotory behavior	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in response to light stimulus	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within sensory perception of sound	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
is_active_in actin cytoskeleton	IBA Inferred from Biological aspect of Ancestor more info
is_active_in actin-based cell projection	IBA Inferred from Biological aspect of Ancestor more info
located_in cell projection	IEA Inferred from Electronic Annotation more info
is_active_in cytoplasm	IBA Inferred from Biological aspect of Ancestor more info
located_in cytoskeleton	IEA Inferred from Electronic Annotation more info
located_in cytosol	TAS Traceable Author Statement more info
is_active_in membrane	IBA Inferred from Biological aspect of Ancestor more info
part_of myosin complex	IEA Inferred from Electronic Annotation more info
located_in stereocilium	IDA Inferred from Direct Assay more info	PubMed
located_in stereocilium bundle	IDA Inferred from Direct Assay more info	PubMed

General protein information

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Preferred Names: unconventional myosin-XV

Names: shaker 2; unconventional myosin-15

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_001103171.1 → NP_001096641.1 unconventional myosin-XV isoform 3

Status: VALIDATED

Description

Transcript Variant: This variant (3) lacks an in-frame exon in the 5' coding region, compared to variant 1, resulting in a shorter protein (isoform 3), compared to isoform 1.

Source sequence(s)

AL596090, AL596386, AY331132

Consensus CDS

CCDS48811.1

UniProtKB/Swiss-Prot

Q9QZZ4

Related

ENSMUSP00000091686.3, ENSMUST00000094135.9

Conserved Domains (7) summary

smart00139
Location:3013 → 3167

MyTH4; Domain in Myosin and Kinesin Tails

smart00242
Location:1201 → 1883

MYSc; Myosin. Large ATPases

smart00295
Location:3177 → 3382

B41; Band 4.1 homologues

cd13201
Location:3378 → 3479

FERM_C_MyoXV; FERM domain C-lobe of Myosin XV (MyoXV/Myo15)

cd14473
Location:3272 → 3372

FERM_B-lobe; FERM domain B-lobe

cd01387
Location:1220 → 1871

MYSc_Myo15; class XV mammal-like myosin, motor domain

cl17036
Location:2834 → 2913

SH3; Src Homology 3 domain superfamily
NM_010862.2 → NP_034992.2 unconventional myosin-XV isoform 1

See identical proteins and their annotated locations for NP_034992.2

Status: VALIDATED

Description

Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1).

Source sequence(s)

AF144095, AL596090, AL596386

Consensus CDS

CCDS24792.1

UniProtKB/Swiss-Prot

A2A637, A2A638, O70395, Q5SX93, Q7TMR5, Q7TMR6, Q9QWL6, Q9QZZ4

Related

ENSMUSP00000071777.3, ENSMUST00000071880.9

Conserved Domains (8) summary

smart00139
Location:3031 → 3185

MyTH4; Domain in Myosin and Kinesin Tails

smart00242
Location:1201 → 1883

MYSc; Myosin. Large ATPases

smart00295
Location:3195 → 3400

B41; Band 4.1 homologues

cd13201
Location:3396 → 3497

FERM_C_MyoXV; FERM domain C-lobe of Myosin XV (MyoXV/Myo15)

cd14473
Location:3290 → 3390

FERM_B-lobe; FERM domain B-lobe

cd01387
Location:1220 → 1871

MYSc_Myo15; class XV mammal-like myosin, motor domain

pfam02050
Location:1870 → 1960

FliJ; Flagellar FliJ protein

cl17036
Location:2852 → 2931

SH3; Src Homology 3 domain superfamily
NM_182698.2 → NP_874357.2 unconventional myosin-XV isoform 2a

Status: VALIDATED

Description

Transcript Variant: This variant (2) lacks two in-frame exons in the 5' coding region, compared to variant 1, resulting in a protein (isoform 2a) with a shorter N-terminus, compared to isoform 1.

Source sequence(s)

AL596386, AY331133

Consensus CDS

CCDS24793.1

UniProtKB/Swiss-Prot

Q9QZZ4

Related

ENSMUSP00000080507.6, ENSMUST00000081823.12

Conserved Domains (7) summary

smart00139
Location:1826 → 1980

MyTH4; Domain in Myosin and Kinesin Tails

smart00242
Location:14 → 696

MYSc; Myosin. Large ATPases

smart00295
Location:1990 → 2195

B41; Band 4.1 homologues

cd13201
Location:2191 → 2292

FERM_C_MyoXV; FERM domain C-lobe of Myosin XV (MyoXV/Myo15)

cd14473
Location:2085 → 2185

FERM_B-lobe; FERM domain B-lobe

cd01387
Location:33 → 684

MYSc_Myo15; class XV mammal-like myosin, motor domain

cl17036
Location:1647 → 1726

SH3; Src Homology 3 domain superfamily

RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCm39 C57BL/6J

Genomic

NC_000077.7 Reference GRCm39 C57BL/6J

Range

60360165..60419195

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_017314331.2 → XP_017169820.1 unconventional myosin-XV isoform X13

UniProtKB/TrEMBL

F6VXK7

Related

ENSMUSP00000120839.2, ENSMUST00000126522.4

Conserved Domains (5) summary

smart00015
Location:632 → 653

IQ; Calmodulin-binding motif

smart00139
Location:772 → 918

MyTH4; Domain in Myosin and Kinesin Tails

PHA03247
Location:1139 → 1377

PHA03247; large tegument protein UL36; Provisional

cl22853
Location:53 → 594

Motor_domain; Myosin and Kinesin motor domain

cl17036
Location:1587 → 1666

SH3; Src Homology 3 domain superfamily
XM_017314330.2 → XP_017169819.1 unconventional myosin-XV isoform X12

UniProtKB/TrEMBL

F6VXK7

Conserved Domains (5) summary

smart00015
Location:632 → 653

IQ; Calmodulin-binding motif

smart00139
Location:772 → 918

MyTH4; Domain in Myosin and Kinesin Tails

PHA03247
Location:1139 → 1377

PHA03247; large tegument protein UL36; Provisional

cl22853
Location:53 → 594

Motor_domain; Myosin and Kinesin motor domain

cl17036
Location:1587 → 1666

SH3; Src Homology 3 domain superfamily
XM_017314329.2 → XP_017169818.1 unconventional myosin-XV isoform X11

UniProtKB/TrEMBL

F6VXK7

Conserved Domains (5) summary

smart00015
Location:632 → 653

IQ; Calmodulin-binding motif

smart00139
Location:1766 → 1920

MyTH4; Domain in Myosin and Kinesin Tails

PHA03247
Location:1139 → 1377

PHA03247; large tegument protein UL36; Provisional

cl22853
Location:53 → 594

Motor_domain; Myosin and Kinesin motor domain

cl17036
Location:1587 → 1666

SH3; Src Homology 3 domain superfamily
XM_017314324.3 → XP_017169813.1 unconventional myosin-XV isoform X6

Conserved Domains (7) summary

smart00015
Location:632 → 653

IQ; Calmodulin-binding motif

smart00139
Location:1739 → 1893

MyTH4; Domain in Myosin and Kinesin Tails

PHA03247
Location:1164 → 1350

PHA03247; large tegument protein UL36; Provisional

cd13201
Location:2104 → 2205

FERM_C_MyoXV; FERM domain C-lobe of Myosin XV (MyoXV/Myo15)

cd14473
Location:1998 → 2098

FERM_B-lobe; FERM domain B-lobe

cl22853
Location:53 → 594

Motor_domain; Myosin and Kinesin motor domain

cl17036
Location:1560 → 1639

SH3; Src Homology 3 domain superfamily
XM_017314320.3 → XP_017169809.1 unconventional myosin-XV isoform X2

Conserved Domains (7) summary

smart00015
Location:632 → 653

IQ; Calmodulin-binding motif

smart00139
Location:1766 → 1920

MyTH4; Domain in Myosin and Kinesin Tails

PHA03247
Location:1139 → 1377

PHA03247; large tegument protein UL36; Provisional

cd13201
Location:2131 → 2232

FERM_C_MyoXV; FERM domain C-lobe of Myosin XV (MyoXV/Myo15)

cd14473
Location:2025 → 2125

FERM_B-lobe; FERM domain B-lobe

cl22853
Location:53 → 594

Motor_domain; Myosin and Kinesin motor domain

cl17036
Location:1587 → 1666

SH3; Src Homology 3 domain superfamily
XM_017314319.3 → XP_017169808.1 unconventional myosin-XV isoform X1

Conserved Domains (7) summary

smart00015
Location:632 → 653

IQ; Calmodulin-binding motif

smart00139
Location:1766 → 1920

MyTH4; Domain in Myosin and Kinesin Tails

PHA03247
Location:1139 → 1377

PHA03247; large tegument protein UL36; Provisional

cd13201
Location:2131 → 2232

FERM_C_MyoXV; FERM domain C-lobe of Myosin XV (MyoXV/Myo15)

cd14473
Location:2025 → 2125

FERM_B-lobe; FERM domain B-lobe

cl22853
Location:53 → 594

Motor_domain; Myosin and Kinesin motor domain

cl17036
Location:1587 → 1666

SH3; Src Homology 3 domain superfamily
XM_017314328.3 → XP_017169817.1 unconventional myosin-XV isoform X10

Conserved Domains (7) summary

smart00015
Location:632 → 653

IQ; Calmodulin-binding motif

smart00139
Location:1704 → 1857

MyTH4; Domain in Myosin and Kinesin Tails

PHA03247
Location:1139 → 1377

PHA03247; large tegument protein UL36; Provisional

cd13201
Location:2068 → 2169

FERM_C_MyoXV; FERM domain C-lobe of Myosin XV (MyoXV/Myo15)

cd14473
Location:1962 → 2062

FERM_B-lobe; FERM domain B-lobe

cl22853
Location:53 → 594

Motor_domain; Myosin and Kinesin motor domain

cl17036
Location:1587 → 1666

SH3; Src Homology 3 domain superfamily
XM_017314321.3 → XP_017169810.1 unconventional myosin-XV isoform X3

Conserved Domains (7) summary

smart00015
Location:632 → 653

IQ; Calmodulin-binding motif

smart00139
Location:1765 → 1919

MyTH4; Domain in Myosin and Kinesin Tails

PHA03247
Location:1138 → 1376

PHA03247; large tegument protein UL36; Provisional

cd13201
Location:2130 → 2231

FERM_C_MyoXV; FERM domain C-lobe of Myosin XV (MyoXV/Myo15)

cd14473
Location:2024 → 2124

FERM_B-lobe; FERM domain B-lobe

cl22853
Location:53 → 594

Motor_domain; Myosin and Kinesin motor domain

cl17036
Location:1586 → 1665

SH3; Src Homology 3 domain superfamily
XM_017314327.3 → XP_017169816.1 unconventional myosin-XV isoform X9

Conserved Domains (8) summary

smart00015
Location:632 → 653

IQ; Calmodulin-binding motif

smart00139
Location:1723 → 1877

MyTH4; Domain in Myosin and Kinesin Tails

PHA03247
Location:1096 → 1334

PHA03247; large tegument protein UL36; Provisional

cd13201
Location:2088 → 2189

FERM_C_MyoXV; FERM domain C-lobe of Myosin XV (MyoXV/Myo15)

cd14473
Location:1982 → 2082

FERM_B-lobe; FERM domain B-lobe

cl22853
Location:53 → 594

Motor_domain; Myosin and Kinesin motor domain

cl02480
Location:772 → 875

MyTH4; MyTH4 domain

cl17036
Location:1544 → 1623

SH3; Src Homology 3 domain superfamily
XM_017314323.3 → XP_017169812.1 unconventional myosin-XV isoform X5

Conserved Domains (7) summary

smart00015
Location:632 → 653

IQ; Calmodulin-binding motif

smart00139
Location:1748 → 1902

MyTH4; Domain in Myosin and Kinesin Tails

PHA03247
Location:1121 → 1359

PHA03247; large tegument protein UL36; Provisional

cd13201
Location:2113 → 2214

FERM_C_MyoXV; FERM domain C-lobe of Myosin XV (MyoXV/Myo15)

cd14473
Location:2007 → 2107

FERM_B-lobe; FERM domain B-lobe

cl22853
Location:53 → 594

Motor_domain; Myosin and Kinesin motor domain

cl17036
Location:1569 → 1648

SH3; Src Homology 3 domain superfamily
XM_017314326.3 → XP_017169815.1 unconventional myosin-XV isoform X8

Conserved Domains (7) summary

smart00015
Location:594 → 615

IQ; Calmodulin-binding motif

smart00139
Location:1728 → 1882

MyTH4; Domain in Myosin and Kinesin Tails

PHA03247
Location:1101 → 1339

PHA03247; large tegument protein UL36; Provisional

cd13201
Location:2093 → 2194

FERM_C_MyoXV; FERM domain C-lobe of Myosin XV (MyoXV/Myo15)

cd14473
Location:1987 → 2087

FERM_B-lobe; FERM domain B-lobe

cl22853
Location:53 → 556

Motor_domain; Myosin and Kinesin motor domain

cl17036
Location:1549 → 1628

SH3; Src Homology 3 domain superfamily
XM_017314325.3 → XP_017169814.1 unconventional myosin-XV isoform X7

Conserved Domains (7) summary

smart00015
Location:594 → 615

IQ; Calmodulin-binding motif

smart00139
Location:1728 → 1882

MyTH4; Domain in Myosin and Kinesin Tails

PHA03247
Location:1101 → 1339

PHA03247; large tegument protein UL36; Provisional

cd13201
Location:2093 → 2194

FERM_C_MyoXV; FERM domain C-lobe of Myosin XV (MyoXV/Myo15)

cd14473
Location:1987 → 2087

FERM_B-lobe; FERM domain B-lobe

cl22853
Location:53 → 556

Motor_domain; Myosin and Kinesin motor domain

cl17036
Location:1549 → 1628

SH3; Src Homology 3 domain superfamily
XM_017314322.3 → XP_017169811.1 unconventional myosin-XV isoform X4

Conserved Domains (7) summary

smart00015
Location:632 → 653

IQ; Calmodulin-binding motif

smart00139
Location:1755 → 1909

MyTH4; Domain in Myosin and Kinesin Tails

PHA03247
Location:1139 → 1377

PHA03247; large tegument protein UL36; Provisional

cd13201
Location:2120 → 2221

FERM_C_MyoXV; FERM domain C-lobe of Myosin XV (MyoXV/Myo15)

cd14473
Location:2014 → 2114

FERM_B-lobe; FERM domain B-lobe

cl22853
Location:53 → 594

Motor_domain; Myosin and Kinesin motor domain

cl17036
Location:1576 → 1655

SH3; Src Homology 3 domain superfamily