kras v-Ki-ras2 Kirsten rat sarcoma viral oncogene homolog [Danio rerio (zebrafish)] - Gene

Summary

Official Symbol: krasprovided by ZNC
Official Full Name: v-Ki-ras2 Kirsten rat sarcoma viral oncogene homologprovided by ZNC
Primary source: ZFIN:ZDB-GENE-040808-67
See related: Ensembl:ENSDARG00000010844 AllianceGenome:ZFIN:ZDB-GENE-040808-67
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Danio rerio
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Actinopterygii; Neopterygii; Teleostei; Ostariophysi; Cypriniformes; Danionidae; Danioninae; Danio
Also known as: K-ras; xRAS2; fa04e08; fc14b12; fc23g10; fj89d12; zgc:85725; wu:fa04e08; wu:fc14b12; wu:fc23g10; wu:fj89d12
Summary: Predicted to enable GDP binding activity; GTP binding activity; and GTPase activity. Acts upstream of or within embryonic cranial skeleton morphogenesis; heart development; and hemopoiesis. Predicted to be located in membrane. Predicted to be active in plasma membrane. Is expressed in brain; heart; liver; pleuroperitoneal region; and trunk musculature. Human ortholog(s) of this gene implicated in several diseases, including Noonan syndrome (multiple); arteriovenous malformations of the brain; cardiofaciocutaneous syndrome (multiple); gastrointestinal system cancer (multiple); and hematologic cancer (multiple). Orthologous to human KRAS (KRAS proto-oncogene, GTPase). [provided by Alliance of Genome Resources, Apr 2022]
Orthologs: all
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Genomic context

Location:: chromosome: 25

Exon count:: 7

Annotation release	Status	Assembly	Chr	Location
106	current	GRCz11 (GCF_000002035.6)	25	NC_007136.7 (31093653..31118904, complement)
105	previous assembly	GRCz10 (GCF_000002035.5)	25	NC_007136.6 (30979385..30985758, complement)

Chromosome 25 - NC_007136.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Genomic Sequence:

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Expression

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See details

Project title: Sequencing the Zebrafish transcriptome from a range of tissues and developmental stages
Description: Sequencing the Zebrafish transcriptome from a range of tissues and developmental stages
BioProject: PRJEB1986
Analysis date: Fri Dec 8 19:48:10 2017

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Lymphatic disorders caused by mosaic, activating KRAS variants respond to MEK inhibition.
Title: Lymphatic disorders caused by mosaic, activating KRAS variants respond to MEK inhibition.
ahctf1 and kras mutations combine to amplify oncogenic stress and restrict liver overgrowth in a zebrafish model of hepatocellular carcinoma.
Title: ahctf1 and kras mutations combine to amplify oncogenic stress and restrict liver overgrowth in a zebrafish model of hepatocellular carcinoma.
KRas-transformed epithelia cells invade and partially dedifferentiate by basal cell extrusion.
Title: KRas-transformed epithelia cells invade and partially dedifferentiate by basal cell extrusion.
Stimulation of hepatocarcinogenesis by activated cholangiocytes via Il17a/f1 pathway in kras transgenic zebrafish model.
Title: Stimulation of hepatocarcinogenesis by activated cholangiocytes via Il17a/f1 pathway in kras transgenic zebrafish model.
Somatic Gain of KRAS Function in the Endothelium Is Sufficient to Cause Vascular Malformations That Require MEK but Not PI3K Signaling.
Title: Somatic Gain of KRAS Function in the Endothelium Is Sufficient to Cause Vascular Malformations That Require MEK but Not PI3K Signaling.
In this study using an inducible kras transgenic zebrafish models for Hepatocellular carcinoma, transcriptomic profiles of oncogenic hepatocytes from larvae, male and female adult fish following a brief induction of oncogenic kras were investigated. Male oncogenic hepatocytes have more severe features than female counterparts.
Title: Transcriptomic analyses of oncogenic hepatocytes reveal common and different molecular pathways of hepatocarcinogenesis in different developmental stages and genders in kras(G12V) transgenic zebrafish.
the lepr mutant background had a higher survival rate and significantly lower muscle-wasting level after tumor induction than the tumor fish in the wild-type background.
Title: Leptin induces muscle wasting in a zebrafish kras-driven hepatocellular carcinoma (HCC) model.
the difference of liver tumorigenesis between female and male fish during early tumorigenesis and long-term tumor progression in previously established inducible and reversible HCC model - the kras(V12) transgenic zebrafish, were investigated.
Title: Males develop faster and more severe hepatocellular carcinoma than females in kras(V12) transgenic zebrafish.
Activating Rho could be beneficial to suppress Kras mutant-induced liver malignancies.
Title: Crosstalk of Ras and Rho: activation of RhoA abates Kras-induced liver tumorigenesis in transgenic zebrafish models.
Kras(V12) transgenic system offers a novel model for understanding hepatocarcinogenesis and a high-throughput screening platform for anti-cancer drugs.
Title: An inducible kras(V12) transgenic zebrafish model for liver tumorigenesis and chemical drug screening.

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General gene information

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Markers

fj89d12.x1 (e-PCR)
- UniSTS:200251

fc23g10.x1 (e-PCR)
- UniSTS:203893

fc14b12.x1 (e-PCR)
- UniSTS:192672

fc14b12.x1 (e-PCR)
- UniSTS:192673

Gene Ontology Provided by ZFIN

Function	Evidence Code	Pubs
enables G protein activity	IEA Inferred from Electronic Annotation more info
enables GDP binding	IBA Inferred from Biological aspect of Ancestor more info
enables GTP binding	IBA Inferred from Biological aspect of Ancestor more info
enables GTP binding	IEA Inferred from Electronic Annotation more info
enables GTPase activity	IBA Inferred from Biological aspect of Ancestor more info
enables GTPase activity	IEA Inferred from Electronic Annotation more info
enables hydrolase activity	IEA Inferred from Electronic Annotation more info
enables nucleotide binding	IEA Inferred from Electronic Annotation more info

Process	Evidence Code	Pubs
involved_in Ras protein signal transduction	IBA Inferred from Biological aspect of Ancestor more info
acts_upstream_of_or_within embryonic cranial skeleton morphogenesis	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within heart development	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within hemopoiesis	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within signal transduction	IEA Inferred from Electronic Annotation more info

Component	Evidence Code	Pubs
located_in membrane	IEA Inferred from Electronic Annotation more info
is_active_in plasma membrane	IBA Inferred from Biological aspect of Ancestor more info

General protein information

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Preferred Names: GTPase KRas

Names: ras-like protein

NP_001003744.1

EC 3.6.5.2

XP_009296393.2

EC 3.6.5.2

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_001003744.2 → NP_001003744.1 GTPase KRas isoform 2

See identical proteins and their annotated locations for NP_001003744.1

Status: VALIDATED

Description

Transcript Variant: This variant (2) lacks an alternate coding exon compared to variant 1, that causes a frameshift. The resulting isoform (2) has a shorter and distinct C-terminus compared to isoform 1.

Source sequence(s)

FO904995, FQ976913

UniProtKB/TrEMBL

F6NPA7, Q1HGL0, Q6AZA4

Related

ENSDARP00000021124.7, ENSDART00000009126.10

Conserved Domains (2) summary

cd04138
Location:3 → 164

H_N_K_Ras_like; Ras GTPase family containing H-Ras,N-Ras and K-Ras4A/4B

smart00010
Location:16 → 166

small_GTPase; Small GTPase of the Ras superfamily; ill-defined subfamily
NM_001362494.1 → NP_001349423.1 GTPase KRas isoform 1

Status: VALIDATED

Description

Transcript Variant: This variant (1) represents the longest transcript and encodes the longer isoform (1).

Source sequence(s)

FO904995, FQ976913

UniProtKB/TrEMBL

A0A2R8Q8S1, A0A8M3AZT3

Conserved Domains (1) summary

cd04138
Location:3 → 164

H_N_K_Ras_like; Ras GTPase family containing H-Ras,N-Ras and K-Ras4A/4B

RNA

NR_155755.1 RNA Sequence

Status: VALIDATED

Description

Transcript Variant: This variant (3) lacks two alternate exons compared to variant 1. This variant is represented as non-coding because the use of the 5'-most expected translational start codon renders the transcript a candidate for nonsense-mediated mRNA decay (NMD).

Source sequence(s)

FO904995, FQ976913

RefSeqs of Annotated Genomes: Danio rerio Annotation Release 106 details...

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCz11 Primary Assembly

Genomic

NC_007136.7 Reference GRCz11 Primary Assembly

Range

31093653..31118904 complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_009298118.3 → XP_009296393.2 GTPase KRas isoform X1

Related

ENSDARP00000147703.1, ENSDART00000188286.1

Conserved Domains (1) summary

cd04138
Location:3 → 164

H_N_K_Ras_like; Ras GTPase family containing H-Ras,N-Ras and K-Ras4A/4B