Pgk1 phosphoglycerate kinase 1 [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: Pgk1provided by MGI
Official Full Name: phosphoglycerate kinase 1provided by MGI
Primary source: MGI:MGI:97555
See related: Ensembl:ENSMUSG00000062070 AllianceGenome:MGI:97555
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as: Pgk-1
Summary: The protein encoded by this gene is a glycolytic enzyme that catalyzes the conversion of 1,3-diphosphoglycerate to 3-phosphoglycerate. Additionally, this protein is secreted by tumor cells where it participates in angiogenesis by functioning to reduce disulfide bonds in the serine protease, plasmin, which consequently leads to the release of the tumor blood vessel inhibitor angiostatin. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. Many pseudogenes of this gene are found throughout the mouse genome. [provided by RefSeq, Jan 2014]
Expression: Ubiquitous expression in placenta adult (RPKM 206.0), liver E18 (RPKM 170.7) and 27 other tissues See more
Orthologs: human all
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Genomic context

Location:: X D; X 47.36 cM

Exon count:: 11

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCm39 (GCF_000001635.27)	X	NC_000086.8 (105230706..105247305)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	X	NC_000086.7 (106187100..106203699)

Chromosome X - NC_000086.8 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Genomic Sequence:

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Expression

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See details

Project title: Mouse ENCODE transcriptome data
Description: RNA profiling data sets generated by the Mouse ENCODE project.
BioProject: PRJNA66167
Publication: PMID 25409824
Analysis date: n/a

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Activation of Pgk1 Results in Reduced Protein Aggregation in Diverse Neurodegenerative Conditions.
Title: Activation of Pgk1 Results in Reduced Protein Aggregation in Diverse Neurodegenerative Conditions.
Git1-PGK1 interaction achieves self-protection against spinal cord ischemia-reperfusion injury by modulating Keap1/Nrf2 signaling.
Title: Git1-PGK1 interaction achieves self-protection against spinal cord ischemia-reperfusion injury by modulating Keap1/Nrf2 signaling.
Not only did supplementary Pgk1 enhance neurite outgrowth of motoneurons in defective cells, but injection of Pgk1 rescued denervation in muscle-specific NogoA-overexpression of zebrafish and an Amyotrophic Lateral Sclerosis mouse model, SOD1 G93A.
Title: Extracellular Pgk1 enhances neurite outgrowth of motoneurons through Nogo66/NgR-independent targeting of NogoA.
Pgk1 overexpression, or treatment with terazosin (an FDA-approved small molecule that binds and activates Pgk1), rescued motor axon phenotypes in SMA zebrafish. We conclude that global bioenergetics pathways can be therapeutically manipulated to ameliorate SMA motor neuron phenotypes in vivo.
Title: Bioenergetic status modulates motor neuron vulnerability and pathogenesis in a zebrafish model of spinal muscular atrophy.
Phosphoglycerate kinase is a moonlighting protein that functions as both a glycolytic enzyme and a disulfide reductase.
Title: Phosphoglycerate kinase acts in tumour angiogenesis as a disulphide reductase.
Purification and characterization of 3-phosphoglycerate kinase from Ehrlich ascites carcinoma cells
Title: Purification and characterization of 3-phosphoglycerate kinase from Ehrlich ascites carcinoma cells.
PGK domain movement and catalysis is regulated by a spring-loaded release mechanism
Title: A spring-loaded release mechanism regulates domain movement and catalysis in phosphoglycerate kinase.
Results indicate that COX-2 suppression by PGK-1 is independent of its catalytic activity.
Title: Nucleotide-binding domain of phosphoglycerate kinase 1 reduces tumor growth by suppressing COX-2 expression.
Together, these data suggest that PGK1 secreted by PCa regulates bone formation at the metastatic site by increasing osteoblastic activity, decreasing osteoclastic function, and expressing an osteoblastic phenotype by PCa cells.
Title: Expression of PGK1 by prostate cancer cells induces bone formation.
Findings indicate that overexpression of PGK-1 in LLC-1 reduces the COX-2 expression, and, in turn, affect PGE2, cell invasion, angiogenesis, and the immune functions, and finally inhibit the tumor progression.
Title: Phosphoglycerate kinase 1-overexpressing lung cancer cells reduce cyclooxygenase 2 expression and promote anti-tumor immunity in vivo.

Submit: New GeneRIF Correction See all GeneRIFs (12)

Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Endonuclease-mediated (1)
Targeted (3) 1 citation

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

Pgk1 (e-PCR), detects polymorphism
- UniSTS:144172

DXMit64 (e-PCR), detects polymorphism
- UniSTS:132169

DXMit64.2 (e-PCR), detects polymorphism
- UniSTS:132170

Pgk1 (e-PCR), detects polymorphism
- UniSTS:531394

Pgk1 (e-PCR), detects polymorphism
- UniSTS:531398

AA892797 (e-PCR)
- UniSTS:250075

Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables ADP binding	IBA Inferred from Biological aspect of Ancestor more info
enables ADP binding	ISO Inferred from Sequence Orthology more info
enables ATP binding	IBA Inferred from Biological aspect of Ancestor more info
enables ATP binding	ISO Inferred from Sequence Orthology more info
enables kinase activity	IEA Inferred from Electronic Annotation more info
enables nucleotide binding	IEA Inferred from Electronic Annotation more info
enables phosphoglycerate kinase activity	IBA Inferred from Biological aspect of Ancestor more info
enables phosphoglycerate kinase activity	IDA Inferred from Direct Assay more info	PubMed
enables phosphoglycerate kinase activity	ISO Inferred from Sequence Orthology more info
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables protein-disulfide reductase (NAD(P)H) activity	ISO Inferred from Sequence Orthology more info
enables transferase activity	IEA Inferred from Electronic Annotation more info

Process	Evidence Code	Pubs
involved_in canonical glycolysis	IGI Inferred from Genetic Interaction more info	PubMed
involved_in cellular response to hypoxia	ISO Inferred from Sequence Orthology more info
involved_in gluconeogenesis	IBA Inferred from Biological aspect of Ancestor more info
involved_in gluconeogenesis	IGI Inferred from Genetic Interaction more info	PubMed
involved_in glyceraldehyde-3-phosphate biosynthetic process	ISO Inferred from Sequence Orthology more info	PubMed
involved_in glycolytic process	IBA Inferred from Biological aspect of Ancestor more info
acts_upstream_of_or_within glycolytic process	IDA Inferred from Direct Assay more info	PubMed
involved_in glycolytic process	ISO Inferred from Sequence Orthology more info
involved_in negative regulation of angiogenesis	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within phosphorylation	IEA Inferred from Electronic Annotation more info
involved_in plasminogen activation	ISO Inferred from Sequence Orthology more info

Component	Evidence Code	Pubs
located_in cytoplasm	ISO Inferred from Sequence Orthology more info	PubMed
is_active_in cytosol	IBA Inferred from Biological aspect of Ancestor more info
is_active_in cytosol	IDA Inferred from Direct Assay more info	PubMed
located_in extracellular space	ISO Inferred from Sequence Orthology more info
located_in membrane raft	ISO Inferred from Sequence Orthology more info

General protein information

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Preferred Names: phosphoglycerate kinase 1

NP_032854.2

EC 2.7.2.3

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.