Cyp27a1 cytochrome P450, family 27, subfamily a, polypeptide 1 [Rattus norvegicus (Norway rat)] - Gene

Summary

Official Symbol: Cyp27a1provided by RGD
Official Full Name: cytochrome P450, family 27, subfamily a, polypeptide 1provided by RGD
Primary source: RGD:727915
See related: Ensembl:ENSRNOG00000017188 AllianceGenome:RGD:727915
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Rattus norvegicus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Rattus
Also known as: Cyp27; P450C27
Summary: Enables Hsp70 protein binding activity; cholesterol 26-hydroxylase activity; and vitamin D3 25-hydroxylase activity. Involved in cholesterol metabolic process. Located in mitochondrial inner membrane. Biomarker of extrahepatic cholestasis. Human ortholog(s) of this gene implicated in cerebrotendinous xanthomatosis. Orthologous to human CYP27A1 (cytochrome P450 family 27 subfamily A member 1). [provided by Alliance of Genome Resources, Apr 2022]
Expression: Biased expression in Liver (RPKM 1095.3), Adrenal (RPKM 195.3) and 4 other tissues See more
Orthologs: human mouse all
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Genomic context

Location:: 9q33

Exon count:: 10

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCr8 (GCF_036323735.1)	9	NC_086027.1 (83712402..83743222)
RS_2023_06	previous assembly	mRatBN7.2 (GCF_015227675.2)	9	NC_051344.1 (76264655..76294551)
106	previous assembly	Rnor_6.0 (GCF_000001895.5)	9	NC_005108.4 (81968285..81998213)

Chromosome 9 - NC_086027.1 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: A rat RNA-Seq transcriptomic BodyMap across 11 organs and 4 developmental stages
Description: 320 RNA samples isolated from 11 organs (adrenal gland, brain, heart, kidney, liver, lung, muscle, spleen, thymus, and testes or uterus) from both sexes of Fischer 344 rats across four developmental stages (2-, 6-, 21-, and 104-weeks-old)
BioProject: PRJNA238328
Publication: PMID 24510058
Analysis date: Mon Jun 6 17:44:12 2016

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Data indicate that inhibition of CYP27A1 activity or knockdown and deletion of the Cyp27a1 gene induced adipocyte differentiation.
Title: De novo synthesis of steroids and oxysterols in adipocytes.
Cyp27a1 is present in glial cells and is regulated in response to LXR activation, consistent with the role played by astrocytes in the control of cholesterol metabolism and homeostasis in the brain in physiological conditions
Title: Expression of sterol 27-hydroxylase in glial cells and its regulation by liver X receptor signaling.
Depleted uranium affects both vitamin D active form and receptor expression and consequently could modulate the expression of cyp24a1 and vitamin D target genes.
Title: In vivo effects of chronic contamination with depleted uranium on vitamin D3 metabolism in rat.

Submit: New GeneRIF Correction

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

RH130485 (e-PCR)
- UniSTS:213769

UniSTS:256607 (e-PCR)
- UniSTS:256607

Cyp27a1 (e-PCR), detects polymorphism
- UniSTS:256607

BM387198 (e-PCR)
- UniSTS:261332

PMC199420P1 (e-PCR)
- UniSTS:271865

Gene Ontology Provided by RGD

Function	Evidence Code	Pubs
enables 3-alpha,7-alpha,12-alpha-trihydroxycholestan-26-al 26-oxidoreductase activity	IEA Inferred from Electronic Annotation more info
enables Hsp70 protein binding	IDA Inferred from Direct Assay more info	PubMed
enables cholestanetetraol 26-dehydrogenase activity	IEA Inferred from Electronic Annotation more info
enables cholestanetriol 26-monooxygenase activity	IEA Inferred from Electronic Annotation more info
enables cholestanetriol 26-monooxygenase activity	ISO Inferred from Sequence Orthology more info
enables cholestanetriol 26-monooxygenase activity	ISS Inferred from Sequence or Structural Similarity more info
enables cholesterol 26-hydroxylase activity	IBA Inferred from Biological aspect of Ancestor more info
enables cholesterol 26-hydroxylase activity	IDA Inferred from Direct Assay more info	PubMed
enables cholesterol 26-hydroxylase activity	IEA Inferred from Electronic Annotation more info
enables cholesterol 26-hydroxylase activity	ISO Inferred from Sequence Orthology more info
enables cholesterol 7-alpha-monooxygenase activity	ISS Inferred from Sequence or Structural Similarity more info
enables cholesterol monooxygenase (side-chain-cleaving) activity	ISS Inferred from Sequence or Structural Similarity more info
enables heme binding	IEA Inferred from Electronic Annotation more info
enables heme binding	ISO Inferred from Sequence Orthology more info
enables heme binding	ISS Inferred from Sequence or Structural Similarity more info
enables iron ion binding	IEA Inferred from Electronic Annotation more info
enables vitamin D3 25-hydroxylase activity	IBA Inferred from Biological aspect of Ancestor more info
enables vitamin D3 25-hydroxylase activity	IDA Inferred from Direct Assay more info	PubMed
enables vitamin D3 25-hydroxylase activity	IEA Inferred from Electronic Annotation more info
enables vitamin D3 25-hydroxylase activity	ISO Inferred from Sequence Orthology more info

Process	Evidence Code	Pubs
involved_in C21-steroid hormone biosynthetic process	ISS Inferred from Sequence or Structural Similarity more info
involved_in bile acid biosynthetic process	IEA Inferred from Electronic Annotation more info
involved_in bile acid biosynthetic process	ISO Inferred from Sequence Orthology more info
involved_in bile acid biosynthetic process	ISS Inferred from Sequence or Structural Similarity more info
involved_in calcitriol biosynthetic process from calciol	IBA Inferred from Biological aspect of Ancestor more info
involved_in calcitriol biosynthetic process from calciol	IEA Inferred from Electronic Annotation more info
involved_in calcitriol biosynthetic process from calciol	ISO Inferred from Sequence Orthology more info
involved_in cholesterol catabolic process	IEA Inferred from Electronic Annotation more info
involved_in cholesterol catabolic process	ISO Inferred from Sequence Orthology more info
involved_in cholesterol catabolic process	ISS Inferred from Sequence or Structural Similarity more info
involved_in cholesterol metabolic process	IBA Inferred from Biological aspect of Ancestor more info
involved_in cholesterol metabolic process	IDA Inferred from Direct Assay more info	PubMed
involved_in steroid catabolic process	TAS Traceable Author Statement more info

Component	Evidence Code	Pubs
located_in mitochondrial inner membrane	IDA Inferred from Direct Assay more info	PubMed
is_active_in mitochondrion	IBA Inferred from Biological aspect of Ancestor more info

General protein information

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Preferred Names: sterol 26-hydroxylase, mitochondrial

Names: 5-beta-cholestane-3-alpha,7-alpha,12-alpha-triol 26-hydroxylase; 5-beta-cholestane-3-alpha,7-alpha,12-alpha-triol 27-hydroxylase; cytochrome P-450c27/25; cytochrome P450 27; sterol 27-hydroxylase; vitamin D(3) 25-hydroxylase

NP_849178.2

EC 1.14.15.15

XP_038939288.1

EC 1.14.15.15

XP_063123046.1

EC 1.14.15.15

XP_063123047.1

EC 1.14.15.15

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_178847.3 → NP_849178.2 sterol 26-hydroxylase, mitochondrial

See identical proteins and their annotated locations for NP_849178.2

Status: VALIDATED

Source sequence(s)

BC061848, CB734352

UniProtKB/Swiss-Prot

P17178, Q64615, Q64639

UniProtKB/TrEMBL

A0A0H2UHN7, A6JVV9

Related

ENSRNOP00000023152.4, ENSRNOT00000023152.6

Conserved Domains (1) summary

cl41757
Location:91 → 527

cytochrome_P450; cytochrome P450 (CYP) superfamily

RefSeqs of Annotated Genomes: GCF_036323735.1-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCr8

Genomic

NC_086027.1 Reference GRCr8

Range

83712402..83743222

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_039083360.2 → XP_038939288.1 sterol 26-hydroxylase, mitochondrial isoform X2

Conserved Domains (1) summary

cl41757
Location:1 → 431

cytochrome_P450; cytochrome P450 (CYP) superfamily
XM_063266976.1 → XP_063123046.1 sterol 26-hydroxylase, mitochondrial isoform X1
XM_063266977.1 → XP_063123047.1 sterol 26-hydroxylase, mitochondrial isoform X2