Wfikkn2 WAP, follistatin/kazal, immunoglobulin, kunitz and netrin domain containing 2 [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: Wfikkn2provided by MGI
Official Full Name: WAP, follistatin/kazal, immunoglobulin, kunitz and netrin domain containing 2provided by MGI
Primary source: MGI:MGI:2669209
See related: Ensembl:ENSMUSG00000044177 AllianceGenome:MGI:2669209
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as: Gasp1; WFIKKNRP; 2610304F08Rik
Summary: Predicted to enable receptor antagonist activity and transforming growth factor beta binding activity. Acts upstream of or within several processes, including negative regulation of DNA binding activity; roof of mouth development; and transforming growth factor beta receptor signaling pathway. Located in extracellular space. Is expressed in several structures, including central nervous system; genitourinary system; limb; liver; and lung. Orthologous to human WFIKKN2 (WAP, follistatin/kazal, immunoglobulin, kunitz and netrin domain containing 2). [provided by Alliance of Genome Resources, Apr 2022]
Expression: Biased expression in thymus adult (RPKM 7.3), limb E14.5 (RPKM 4.3) and 14 other tissues See more
Orthologs: human all
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Genomic context

Location:: 11 D; 11 58.9 cM

Exon count:: 9

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCm39 (GCF_000001635.27)	11	NC_000077.7 (94126777..94138613, complement)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	11	NC_000077.6 (94235951..94247793, complement)

Chromosome 11 - NC_000077.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

results indicate that both WFIKKN and follistatin utilize their FSDs to block the type II receptor but do so via different binding interactions.
Title: Crystal structure of the WFIKKN2 follistatin domain reveals insight into how it inhibits growth differentiation factor 8 (GDF8) and GDF11.
Taken together, this study provides evidence that GASP1 regulates CB1 receptor downregulation in vivo, and that postendocytic receptor trafficking has a key role in the development of tolerance to WIN55,212-2.
Title: Differential regulation of behavioral tolerance to WIN55,212-2 by GASP1.
Report of the identification of growth and differentiation factor-associated serum protein-1 (GASP-1) associated with endogenous myostatin in normal serum
Title: Regulation of myostatin in vivo by growth and differentiation factor-associated serum protein-1: a novel protein with protease inhibitor and follistatin domains.

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Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Targeted (3) 1 citation
Endonuclease-mediated (1)

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

MHAa88g3.seq (e-PCR)
- UniSTS:125440

AY100450 (e-PCR), detects polymorphism
- UniSTS:265049

Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables enzyme inhibitor activity	IEA Inferred from Electronic Annotation more info
enables metalloendopeptidase inhibitor activity	IEA Inferred from Electronic Annotation more info
enables peptidase inhibitor activity	IEA Inferred from Electronic Annotation more info
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables receptor antagonist activity	IBA Inferred from Biological aspect of Ancestor more info
enables receptor antagonist activity	ISO Inferred from Sequence Orthology more info
enables serine-type endopeptidase inhibitor activity	IEA Inferred from Electronic Annotation more info
enables transforming growth factor beta binding	IBA Inferred from Biological aspect of Ancestor more info
enables transforming growth factor beta binding	ISO Inferred from Sequence Orthology more info

Process	Evidence Code	Pubs
acts_upstream_of_or_within muscle cell development	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within negative regulation of DNA binding	IDA Inferred from Direct Assay more info	PubMed
acts_upstream_of_or_within negative regulation of peptidase activity	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within negative regulation of protein binding	IDA Inferred from Direct Assay more info	PubMed
involved_in negative regulation of transforming growth factor beta receptor signaling pathway	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within roof of mouth development	IGI Inferred from Genetic Interaction more info	PubMed
acts_upstream_of_or_within skeletal system development	IGI Inferred from Genetic Interaction more info	PubMed
involved_in transforming growth factor beta receptor signaling pathway	IBA Inferred from Biological aspect of Ancestor more info
acts_upstream_of_or_within transforming growth factor beta receptor signaling pathway	IDA Inferred from Direct Assay more info	PubMed

Component	Evidence Code	Pubs
located_in extracellular region	IDA Inferred from Direct Assay more info	PubMed
is_active_in extracellular space	IBA Inferred from Biological aspect of Ancestor more info
located_in extracellular space	IDA Inferred from Direct Assay more info	PubMed
located_in extracellular space	ISO Inferred from Sequence Orthology more info

General protein information

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Preferred Names: WAP, Kazal, immunoglobulin, Kunitz and NTR domain-containing protein 2

Names: GASP-1; growth and differentiation factor-associated serum protein 1; mGASP-1

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_181819.2 → NP_861540.2 WAP, Kazal, immunoglobulin, Kunitz and NTR domain-containing protein 2 precursor

See identical proteins and their annotated locations for NP_861540.2

Status: VALIDATED

Source sequence(s)

AL645846

Consensus CDS

CCDS25251.1

UniProtKB/Swiss-Prot

Q5SUS5, Q7TQN3

UniProtKB/TrEMBL

K4HPC4

Related

ENSMUSP00000053238.4, ENSMUST00000061469.4

Conserved Domains (6) summary

cd05765
Location:219 → 299

Ig_3; Subgroup of the immunoglobulin (Ig) superfamily

cd00104
Location:133 → 169

KAZAL_FS; Kazal type serine protease inhibitors and follistatin-like domains. Kazal inhibitors inhibit serine proteases, such as, trypsin, chyomotrypsin, avian ovomucoids, and elastases. The inhibitory domain has one reactive site peptide bond, which serves the ...

cd00109
Location:323 → 374

KU; BPTI/Kunitz family of serine protease inhibitors; Structure is a disulfide rich alpha+beta fold. BPTI (bovine pancreatic trypsin inhibitor) is an extensively studied model structure.

cd03575
Location:450 → 558

NTR_WFIKKN; NTR domain, WFIKKN subfamily; WFIKKN proteins contain a C-terminal NTR domain and are putative secreted proteins which may be multivalent protease inhibitors that act on serine proteases as well as metalloproteases. Human WFIKKN and a related protein ...

pfam00014
Location:380 → 431

Kunitz_BPTI; Kunitz/Bovine pancreatic trypsin inhibitor domain

pfam00095
Location:37 → 86

WAP; WAP-type (Whey Acidic Protein) 'four-disulfide core'

RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCm39 C57BL/6J

Genomic

NC_000077.7 Reference GRCm39 C57BL/6J

Range

94126777..94138613 complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_011249063.4 → XP_011247365.1 WAP, Kazal, immunoglobulin, Kunitz and NTR domain-containing protein 2 isoform X2

See identical proteins and their annotated locations for XP_011247365.1

UniProtKB/Swiss-Prot

Q5SUS5, Q7TQN3

UniProtKB/TrEMBL

K4HPC4

Conserved Domains (6) summary

cd05765
Location:219 → 299

Ig_3; Subgroup of the immunoglobulin (Ig) superfamily

cd00104
Location:133 → 169

KAZAL_FS; Kazal type serine protease inhibitors and follistatin-like domains. Kazal inhibitors inhibit serine proteases, such as, trypsin, chyomotrypsin, avian ovomucoids, and elastases. The inhibitory domain has one reactive site peptide bond, which serves the ...

cd00109
Location:323 → 374

KU; BPTI/Kunitz family of serine protease inhibitors; Structure is a disulfide rich alpha+beta fold. BPTI (bovine pancreatic trypsin inhibitor) is an extensively studied model structure.

cd03575
Location:450 → 558

NTR_WFIKKN; NTR domain, WFIKKN subfamily; WFIKKN proteins contain a C-terminal NTR domain and are putative secreted proteins which may be multivalent protease inhibitors that act on serine proteases as well as metalloproteases. Human WFIKKN and a related protein ...

pfam00014
Location:380 → 431

Kunitz_BPTI; Kunitz/Bovine pancreatic trypsin inhibitor domain

pfam00095
Location:37 → 86

WAP; WAP-type (Whey Acidic Protein) 'four-disulfide core'
XM_006533500.5 → XP_006533563.1 WAP, Kazal, immunoglobulin, Kunitz and NTR domain-containing protein 2 isoform X2

See identical proteins and their annotated locations for XP_006533563.1

UniProtKB/Swiss-Prot

Q5SUS5, Q7TQN3

UniProtKB/TrEMBL

K4HPC4

Conserved Domains (6) summary

cd05765
Location:219 → 299

Ig_3; Subgroup of the immunoglobulin (Ig) superfamily

cd00104
Location:133 → 169

KAZAL_FS; Kazal type serine protease inhibitors and follistatin-like domains. Kazal inhibitors inhibit serine proteases, such as, trypsin, chyomotrypsin, avian ovomucoids, and elastases. The inhibitory domain has one reactive site peptide bond, which serves the ...

cd00109
Location:323 → 374

KU; BPTI/Kunitz family of serine protease inhibitors; Structure is a disulfide rich alpha+beta fold. BPTI (bovine pancreatic trypsin inhibitor) is an extensively studied model structure.

cd03575
Location:450 → 558

NTR_WFIKKN; NTR domain, WFIKKN subfamily; WFIKKN proteins contain a C-terminal NTR domain and are putative secreted proteins which may be multivalent protease inhibitors that act on serine proteases as well as metalloproteases. Human WFIKKN and a related protein ...

pfam00014
Location:380 → 431

Kunitz_BPTI; Kunitz/Bovine pancreatic trypsin inhibitor domain

pfam00095
Location:37 → 86

WAP; WAP-type (Whey Acidic Protein) 'four-disulfide core'
XM_006533497.5 → XP_006533560.1 WAP, Kazal, immunoglobulin, Kunitz and NTR domain-containing protein 2 isoform X1

UniProtKB/TrEMBL

K4HPC4

Conserved Domains (6) summary

cd05765
Location:233 → 313

Ig_3; Subgroup of the immunoglobulin (Ig) superfamily

cd00104
Location:147 → 183

KAZAL_FS; Kazal type serine protease inhibitors and follistatin-like domains. Kazal inhibitors inhibit serine proteases, such as, trypsin, chyomotrypsin, avian ovomucoids, and elastases. The inhibitory domain has one reactive site peptide bond, which serves the ...

cd00109
Location:337 → 388

KU; BPTI/Kunitz family of serine protease inhibitors; Structure is a disulfide rich alpha+beta fold. BPTI (bovine pancreatic trypsin inhibitor) is an extensively studied model structure.

cd03575
Location:464 → 572

NTR_WFIKKN; NTR domain, WFIKKN subfamily; WFIKKN proteins contain a C-terminal NTR domain and are putative secreted proteins which may be multivalent protease inhibitors that act on serine proteases as well as metalloproteases. Human WFIKKN and a related protein ...

pfam00014
Location:394 → 445

Kunitz_BPTI; Kunitz/Bovine pancreatic trypsin inhibitor domain

pfam00095
Location:51 → 100

WAP; WAP-type (Whey Acidic Protein) 'four-disulfide core'
XM_006533501.5 → XP_006533564.1 WAP, Kazal, immunoglobulin, Kunitz and NTR domain-containing protein 2 isoform X2

See identical proteins and their annotated locations for XP_006533564.1

UniProtKB/Swiss-Prot

Q5SUS5, Q7TQN3

UniProtKB/TrEMBL

K4HPC4

Conserved Domains (6) summary

cd05765
Location:219 → 299

Ig_3; Subgroup of the immunoglobulin (Ig) superfamily

cd00104
Location:133 → 169

KAZAL_FS; Kazal type serine protease inhibitors and follistatin-like domains. Kazal inhibitors inhibit serine proteases, such as, trypsin, chyomotrypsin, avian ovomucoids, and elastases. The inhibitory domain has one reactive site peptide bond, which serves the ...

cd00109
Location:323 → 374

KU; BPTI/Kunitz family of serine protease inhibitors; Structure is a disulfide rich alpha+beta fold. BPTI (bovine pancreatic trypsin inhibitor) is an extensively studied model structure.

cd03575
Location:450 → 558

NTR_WFIKKN; NTR domain, WFIKKN subfamily; WFIKKN proteins contain a C-terminal NTR domain and are putative secreted proteins which may be multivalent protease inhibitors that act on serine proteases as well as metalloproteases. Human WFIKKN and a related protein ...

pfam00014
Location:380 → 431

Kunitz_BPTI; Kunitz/Bovine pancreatic trypsin inhibitor domain

pfam00095
Location:37 → 86

WAP; WAP-type (Whey Acidic Protein) 'four-disulfide core'
XM_006533498.5 → XP_006533561.1 WAP, Kazal, immunoglobulin, Kunitz and NTR domain-containing protein 2 isoform X2

See identical proteins and their annotated locations for XP_006533561.1

UniProtKB/Swiss-Prot

Q5SUS5, Q7TQN3

UniProtKB/TrEMBL

K4HPC4

Conserved Domains (6) summary

cd05765
Location:219 → 299

Ig_3; Subgroup of the immunoglobulin (Ig) superfamily

cd00104
Location:133 → 169

KAZAL_FS; Kazal type serine protease inhibitors and follistatin-like domains. Kazal inhibitors inhibit serine proteases, such as, trypsin, chyomotrypsin, avian ovomucoids, and elastases. The inhibitory domain has one reactive site peptide bond, which serves the ...

cd00109
Location:323 → 374

KU; BPTI/Kunitz family of serine protease inhibitors; Structure is a disulfide rich alpha+beta fold. BPTI (bovine pancreatic trypsin inhibitor) is an extensively studied model structure.

cd03575
Location:450 → 558

NTR_WFIKKN; NTR domain, WFIKKN subfamily; WFIKKN proteins contain a C-terminal NTR domain and are putative secreted proteins which may be multivalent protease inhibitors that act on serine proteases as well as metalloproteases. Human WFIKKN and a related protein ...

pfam00014
Location:380 → 431

Kunitz_BPTI; Kunitz/Bovine pancreatic trypsin inhibitor domain

pfam00095
Location:37 → 86

WAP; WAP-type (Whey Acidic Protein) 'four-disulfide core'
XM_006533502.4 → XP_006533565.1 WAP, Kazal, immunoglobulin, Kunitz and NTR domain-containing protein 2 isoform X2

See identical proteins and their annotated locations for XP_006533565.1

UniProtKB/Swiss-Prot

Q5SUS5, Q7TQN3

UniProtKB/TrEMBL

K4HPC4

Conserved Domains (6) summary

cd05765
Location:219 → 299

Ig_3; Subgroup of the immunoglobulin (Ig) superfamily

cd00104
Location:133 → 169

KAZAL_FS; Kazal type serine protease inhibitors and follistatin-like domains. Kazal inhibitors inhibit serine proteases, such as, trypsin, chyomotrypsin, avian ovomucoids, and elastases. The inhibitory domain has one reactive site peptide bond, which serves the ...

cd00109
Location:323 → 374

KU; BPTI/Kunitz family of serine protease inhibitors; Structure is a disulfide rich alpha+beta fold. BPTI (bovine pancreatic trypsin inhibitor) is an extensively studied model structure.

cd03575
Location:450 → 558

NTR_WFIKKN; NTR domain, WFIKKN subfamily; WFIKKN proteins contain a C-terminal NTR domain and are putative secreted proteins which may be multivalent protease inhibitors that act on serine proteases as well as metalloproteases. Human WFIKKN and a related protein ...

pfam00014
Location:380 → 431

Kunitz_BPTI; Kunitz/Bovine pancreatic trypsin inhibitor domain

pfam00095
Location:37 → 86

WAP; WAP-type (Whey Acidic Protein) 'four-disulfide core'
XM_006533499.5 → XP_006533562.1 WAP, Kazal, immunoglobulin, Kunitz and NTR domain-containing protein 2 isoform X2

See identical proteins and their annotated locations for XP_006533562.1

UniProtKB/Swiss-Prot

Q5SUS5, Q7TQN3

UniProtKB/TrEMBL

K4HPC4

Conserved Domains (6) summary

cd05765
Location:219 → 299

Ig_3; Subgroup of the immunoglobulin (Ig) superfamily

cd00104
Location:133 → 169

KAZAL_FS; Kazal type serine protease inhibitors and follistatin-like domains. Kazal inhibitors inhibit serine proteases, such as, trypsin, chyomotrypsin, avian ovomucoids, and elastases. The inhibitory domain has one reactive site peptide bond, which serves the ...

cd00109
Location:323 → 374

KU; BPTI/Kunitz family of serine protease inhibitors; Structure is a disulfide rich alpha+beta fold. BPTI (bovine pancreatic trypsin inhibitor) is an extensively studied model structure.

cd03575
Location:450 → 558

NTR_WFIKKN; NTR domain, WFIKKN subfamily; WFIKKN proteins contain a C-terminal NTR domain and are putative secreted proteins which may be multivalent protease inhibitors that act on serine proteases as well as metalloproteases. Human WFIKKN and a related protein ...

pfam00014
Location:380 → 431

Kunitz_BPTI; Kunitz/Bovine pancreatic trypsin inhibitor domain

pfam00095
Location:37 → 86

WAP; WAP-type (Whey Acidic Protein) 'four-disulfide core'