Gpsm2 G-protein signalling modulator 2 (AGS3-like, C. elegans) [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: Gpsm2provided by MGI
Official Full Name: G-protein signalling modulator 2 (AGS3-like, C. elegans)provided by MGI
Primary source: MGI:MGI:1923373
See related: Ensembl:ENSMUSG00000027883 AllianceGenome:MGI:1923373
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as: LGN; Pins; 6230410J09Rik
Summary: Enables G-protein alpha-subunit binding activity and GDP-dissociation inhibitor activity. Acts upstream of or within establishment of mitotic spindle orientation and lung epithelial cell differentiation. Located in apical part of cell and lateral cell cortex. Is expressed in several structures, including gonad; lung; nervous system; sensory organ; and spleen. Human ortholog(s) of this gene implicated in sensorineural hearing loss. Orthologous to human GPSM2 (G protein signaling modulator 2). [provided by Alliance of Genome Resources, Apr 2022]
Expression: Broad expression in CNS E11.5 (RPKM 14.3), limb E14.5 (RPKM 14.1) and 25 other tissues See more
Orthologs: human all
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Genomic context

Location:: 3 F3; 3 47.53 cM

Exon count:: 15

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCm39 (GCF_000001635.27)	3	NC_000069.7 (108585954..108629637, complement)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	3	NC_000069.6 (108678270..108722331, complement)

Chromosome 3 - NC_000069.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Genomic Sequence:

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Expression

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See details

Project title: Mouse ENCODE transcriptome data
Description: RNA profiling data sets generated by the Mouse ENCODE project.
BioProject: PRJNA66167
Publication: PMID 25409824
Analysis date: n/a

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

AGS3 antagonizes LGN to balance oriented cell divisions and cell fate choices in mammalian epidermis.
Title: AGS3 antagonizes LGN to balance oriented cell divisions and cell fate choices in mammalian epidermis.
RGS12 polarizes the GPSM2-GNAI complex to organize and elongate stereocilia in sensory hair cells.
Title: RGS12 polarizes the GPSM2-GNAI complex to organize and elongate stereocilia in sensory hair cells.
Legumain-deficient macrophages promote senescence of tumor cells by sustaining JAK1/STAT1 activation.
Title: Legumain-deficient macrophages promote senescence of tumor cells by sustaining JAK1/STAT1 activation.
GPSM2-GNAI confers on the first row its unique tallest identity and participates in generating differential row identity across the hair bundle.
Title: GPSM2-GNAI Specifies the Tallest Stereocilia and Defines Hair Bundle Row Identity.
Mutation of the mouse orthologue of GPSM2 affects actin-rich stereocilia elongation in auditory and vestibular hair cells, causing deafness and balance defects. Gpsm2 defines an approximately 200 nm nanodomain at the tips of stereocilia and this localization requires the presence of Galphai3, myosin 15 and whirlin. Loss of Gpsm2 leads to decreased outgrowth and a disruption of actin dynamics in neuronal growth cones.
Title: Defective Gpsm2/Gα(i3) signalling disrupts stereocilia development and growth cone actin dynamics in Chudley-McCullough syndrome.
LGN in complex with the asymmetric domain of Inscuteable, which reveals a tetrameric arrangement of intertwined molecules.
Title: Insc:LGN tetramers promote asymmetric divisions of mammary stem cells.
LGN and Galphai participate in a long-inferred signal that originates outside the bundle to model its staircase-like architecture, a property that is essential for direction sensitivity to mechanical deflection and hearing.
Title: A link between planar polarity and staircase-like bundle architecture in hair cells.
Kinocilium is essential for proper localization of Lgn, as well as Gai and aPKC, suggesting that cilium function plays a role in positioning of apical proteins critical for hearing.
Title: The GPSM2/LGN GoLoco motifs are essential for hearing.
these results show a context-dependent function for LGN.
Title: The LGN protein promotes planar proliferative divisions in the neocortex but apicobasal asymmetric terminal divisions in the retina.
support for the hitherto untested model that Par3-mInsc and Galphai3 act cooperatively to polarize LGN and promote perpendicular divisions
Title: Par3-mInsc and Gαi3 cooperate to promote oriented epidermal cell divisions through LGN.

Submit: New GeneRIF Correction See all GeneRIFs (16)

Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Endonuclease-mediated (1)
Targeted (5) 1 citation

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables G-protein alpha-subunit binding	IBA Inferred from Biological aspect of Ancestor more info
enables G-protein alpha-subunit binding	IPI Inferred from Physical Interaction more info	PubMed
enables GDP-dissociation inhibitor activity	IBA Inferred from Biological aspect of Ancestor more info
enables GDP-dissociation inhibitor activity	IDA Inferred from Direct Assay more info	PubMed
enables GTPase regulator activity	IEA Inferred from Electronic Annotation more info
enables dynein complex binding	ISO Inferred from Sequence Orthology more info
enables identical protein binding	ISO Inferred from Sequence Orthology more info
enables nucleotide binding	IEA Inferred from Electronic Annotation more info
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables protein domain specific binding	ISO Inferred from Sequence Orthology more info

Process	Evidence Code	Pubs
acts_upstream_of_or_within cell cycle	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within cell division	IEA Inferred from Electronic Annotation more info
involved_in establishment of mitotic spindle orientation	IBA Inferred from Biological aspect of Ancestor more info
acts_upstream_of_or_within establishment of mitotic spindle orientation	IMP Inferred from Mutant Phenotype more info
involved_in establishment of mitotic spindle orientation	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within lung epithelial cell differentiation	IMP Inferred from Mutant Phenotype more info
involved_in maintenance of centrosome location	ISO Inferred from Sequence Orthology more info
involved_in mitotic spindle organization	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of protein localization to cell cortex	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of spindle assembly	ISO Inferred from Sequence Orthology more info
involved_in regulation of mitotic spindle organization	ISO Inferred from Sequence Orthology more info

Component	Evidence Code	Pubs
located_in apical part of cell	IDA Inferred from Direct Assay more info
is_active_in cell cortex	IBA Inferred from Biological aspect of Ancestor more info
colocalizes_with cell cortex	ISO Inferred from Sequence Orthology more info
located_in cell cortex	ISO Inferred from Sequence Orthology more info
located_in cell cortex region	ISO Inferred from Sequence Orthology more info
located_in centrosome	ISO Inferred from Sequence Orthology more info
located_in cytoplasm	ISO Inferred from Sequence Orthology more info
located_in cytoskeleton	IEA Inferred from Electronic Annotation more info
located_in cytosol	ISO Inferred from Sequence Orthology more info
located_in lateral cell cortex	IDA Inferred from Direct Assay more info	PubMed
located_in lateral cell cortex	ISO Inferred from Sequence Orthology more info
located_in membrane	IEA Inferred from Electronic Annotation more info
colocalizes_with mitotic spindle pole	ISO Inferred from Sequence Orthology more info
located_in mitotic spindle pole	ISO Inferred from Sequence Orthology more info
located_in plasma membrane	IEA Inferred from Electronic Annotation more info
part_of protein-containing complex	ISO Inferred from Sequence Orthology more info

General protein information

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Preferred Names: G-protein-signaling modulator 2

Names: pins homolog (Drosophia)

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_029522.2 → NP_083798.2 G-protein-signaling modulator 2

See identical proteins and their annotated locations for NP_083798.2

Status: VALIDATED

Source sequence(s)

AK143552, AY081187

Consensus CDS

CCDS51048.1

UniProtKB/Swiss-Prot

Q8BLX3, Q8VDU0

UniProtKB/TrEMBL

Q3UPG3

Related

ENSMUSP00000029482.9, ENSMUST00000029482.16

Conserved Domains (6) summary

smart00390
Location:490 → 512

GoLoco; LGN motif, putative GEFs specific for G-alpha GTPases

sd00006
Location:243 → 270

TPR; TPR repeat [structural motif]

pfam00515
Location:243 → 274

TPR_1; Tetratricopeptide repeat

pfam02188
Location:629 → 650

GoLoco; GoLoco motif

pfam13176
Location:284 → 318

TPR_7; Tetratricopeptide repeat

pfam13424
Location:243 → 314

TPR_12; Tetratricopeptide repeat

RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCm39 C57BL/6J

Genomic

NC_000069.7 Reference GRCm39 C57BL/6J

Range

108585954..108629637 complement

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_006502256.5 → XP_006502319.1 G-protein-signaling modulator 2 isoform X1

Conserved Domains (5) summary

smart00390
Location:471 → 493

GoLoco; LGN motif, putative GEFs specific for G-alpha GTPases

COG0457
Location:8 → 267

TPR; Tetratricopeptide (TPR) repeat [General function prediction only]

sd00006
Location:224 → 251

TPR; TPR repeat [structural motif]

pfam13424
Location:224 → 295

TPR_12; Tetratricopeptide repeat

pfam02188
Location:611 → 631

GoLoco; GoLoco motif