Cpn1 carboxypeptidase N, polypeptide 1 [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: Cpn1provided by MGI
Official Full Name: carboxypeptidase N, polypeptide 1provided by MGI
Primary source: MGI:MGI:2135874
See related: Ensembl:ENSMUSG00000025196 AllianceGenome:MGI:2135874
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as: CPN; 0610011F20Rik
Summary: Enables carboxypeptidase activity. Predicted to be involved in bradykinin catabolic process; protein processing; and response to glucocorticoid. Predicted to be located in extracellular region. Predicted to be active in extracellular space. Is expressed in several structures, including gonad; gut; liver; neural tube; and yolk sac. Human ortholog(s) of this gene implicated in carboxypeptidase N deficiency. Orthologous to human CPN1 (carboxypeptidase N subunit 1). [provided by Alliance of Genome Resources, Apr 2022]
Expression: Biased expression in liver adult (RPKM 130.6), liver E18 (RPKM 106.8) and 6 other tissues See more
Orthologs: human all
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Genomic context

Location:: 19 C3; 19 36.68 cM

Exon count:: 9

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCm39 (GCF_000001635.27)	19	NC_000085.7 (43944746..43974990, complement)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	19	NC_000085.6 (43956307..43986551, complement)

Chromosome 19 - NC_000085.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Genomic Sequence:

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Expression

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See details

Project title: Mouse ENCODE transcriptome data
Description: RNA profiling data sets generated by the Mouse ENCODE project.
BioProject: PRJNA66167
Publication: PMID 25409824
Analysis date: n/a

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Both plasma basic carboxypeptidases, carboxypeptidase B2 and carboxypeptidase N, regulate vascular leakage activity in mice.
Title: Both plasma basic carboxypeptidases, carboxypeptidase B2 and carboxypeptidase N, regulate vascular leakage activity in mice.
plays a role in experimental autoimmune encephalomyelitis development and progression
Title: Carboxypeptidase N-deficient mice present with polymorphic disease phenotypes on induction of experimental autoimmune encephalomyelitis.
These results indicate that carboxypeptidases contribute to the pathogenesis of experimental cerebral malaria.
Title: Deletion of carboxypeptidase N delays onset of experimental cerebral malaria.
we report the generation of a mouse with complete CPN deficiency by targeted disruption of the CPN1 gene
Title: Targeted disruption of the gene encoding the murine small subunit of carboxypeptidase N (CPN1) causes susceptibility to C5a anaphylatoxin-mediated shock.
carboxypeptidase N regulates the biologic activity of SDF-1alpha by reducing the chemokine-specific activity
Title: Identification of carboxypeptidase N as an enzyme responsible for C-terminal cleavage of stromal cell-derived factor-1alpha in the circulation.

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Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Targeted (3) 1 citation

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

Cpn1 (e-PCR), detects polymorphism
- UniSTS:516131

Cpn1 (e-PCR), detects polymorphism
- UniSTS:507971

NoName (e-PCR)
- UniSTS:237952

Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables carboxypeptidase activity	IDA Inferred from Direct Assay more info	PubMed
enables carboxypeptidase activity	ISO Inferred from Sequence Orthology more info
enables hydrolase activity	IEA Inferred from Electronic Annotation more info
enables metal ion binding	IEA Inferred from Electronic Annotation more info
enables metallocarboxypeptidase activity	IBA Inferred from Biological aspect of Ancestor more info
enables metallopeptidase activity	IEA Inferred from Electronic Annotation more info
enables peptidase activity	IEA Inferred from Electronic Annotation more info
enables zinc ion binding	IEA Inferred from Electronic Annotation more info

Process	Evidence Code	Pubs
involved_in bradykinin catabolic process	ISO Inferred from Sequence Orthology more info
involved_in peptide metabolic process	IBA Inferred from Biological aspect of Ancestor more info
involved_in protein catabolic process	ISO Inferred from Sequence Orthology more info
involved_in protein processing	IBA Inferred from Biological aspect of Ancestor more info
acts_upstream_of_or_within proteolysis	IEA Inferred from Electronic Annotation more info
involved_in proteolysis	IEA Inferred from Electronic Annotation more info
involved_in response to glucocorticoid	ISO Inferred from Sequence Orthology more info

Component	Evidence Code	Pubs
located_in extracellular region	IEA Inferred from Electronic Annotation more info
is_active_in extracellular space	IBA Inferred from Biological aspect of Ancestor more info
located_in extracellular space	ISO Inferred from Sequence Orthology more info

General protein information

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Preferred Names: carboxypeptidase N catalytic chain

Names: anaphylatoxin inactivator; carboxypeptidase N small subunit; carboxypeptidase N, polypeptide 1, 50kD; kininase I

NP_109628.1

EC 3.4.17.3

XP_011245709.1

EC 3.4.17.3

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_030703.2 → NP_109628.1 carboxypeptidase N catalytic chain precursor

See identical proteins and their annotated locations for NP_109628.1

Status: VALIDATED

Source sequence(s)

AF326477

Consensus CDS

CCDS29840.1

UniProtKB/Swiss-Prot

Q91WM9, Q9JJN5

Related

ENSMUSP00000026210.5, ENSMUST00000026210.5

Conserved Domains (2) summary

cd11308
Location:341 → 415

Peptidase_M14NE-CP-C_like; Peptidase associated domain: C-terminal domain of M14 N/E carboxypeptidase; putative folding, regulation, or interaction domain

cl11393
Location:25 → 337

Peptidase_M14_like; M14 family of metallocarboxypeptidases and related proteins

RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCm39 C57BL/6J

Genomic

NC_000085.7 Reference GRCm39 C57BL/6J

Range

43944746..43974990 complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_011247407.1 → XP_011245709.1 carboxypeptidase N catalytic chain isoform X1

Conserved Domains (2) summary

cd11308
Location:347 → 421

Peptidase_M14NE-CP-C_like; Peptidase associated domain: C-terminal domain of M14 N/E carboxypeptidase; putative folding, regulation, or interaction domain

cl11393
Location:25 → 343

Peptidase_M14_like; M14 family of metallocarboxypeptidases and related proteins