UBE2F ubiquitin conjugating enzyme E2 F (putative) [Homo sapiens (human)] - Gene

Summary

Official Symbol: UBE2Fprovided by HGNC
Official Full Name: ubiquitin conjugating enzyme E2 F (putative)provided by HGNC
Primary source: HGNC:HGNC:12480
See related: Ensembl:ENSG00000184182 MIM:617700; AllianceGenome:HGNC:12480
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Homo sapiens
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo
Also known as: NCE2
Summary: Enables NEDD8 conjugating enzyme activity. Involved in protein neddylation. Predicted to be located in cytosol. Predicted to be active in nucleus. [provided by Alliance of Genome Resources, Apr 2022]
Expression: Ubiquitous expression in bone marrow (RPKM 11.8), thyroid (RPKM 10.6) and 25 other tissues See more
Orthologs: mouse all
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Genomic context

Location:: 2q37.3

Exon count:: 12

Annotation release	Status	Assembly	Chr	Location
RS_2023_10	current	GRCh38.p14 (GCF_000001405.40)	2	NC_000002.12 (237967014..238042782)
RS_2023_10	current	T2T-CHM13v2.0 (GCF_009914755.1)	2	NC_060926.1 (238457016..238532785)
105.20220307	previous assembly	GRCh37.p13 (GCF_000001405.25)	2	NC_000002.11 (238875656..238951423)

Chromosome 2 - NC_000002.12 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: HPA RNA-seq normal tissues
Description: RNA-seq was performed of tissue samples from 95 human individuals representing 27 different tissues in order to determine tissue-specificity of all protein-coding genes
BioProject: PRJEB4337
Publication: PMID 24309898
Analysis date: Wed Apr 4 07:08:55 2018

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

NEDD8-conjugating enzyme E2 UBE2F confers radiation resistance by protecting lung cancer cells from apoptosis.
Title: NEDD8-conjugating enzyme E2 UBE2F confers radiation resistance by protecting lung cancer cells from apoptosis.
Induction of NEDD8-conjugating enzyme E2 UBE2F by platinum protects lung cancer cells from apoptosis and confers to platinum-insensitivity.
Title: Induction of NEDD8-conjugating enzyme E2 UBE2F by platinum protects lung cancer cells from apoptosis and confers to platinum-insensitivity.
Study establishes a negative regulatory axis between two neddylation E2s with UBE2M ubiquitylating UBE2F, and two CRLs with CRL3 inactivating CRL5.
Title: UBE2M Is a Stress-Inducible Dual E2 for Neddylation and Ubiquitylation that Promotes Targeted Degradation of UBE2F.
By ubiquitylating and degrading NOXA through activating CRL5, UBE2F selectively promotes lung cancer cell survival and could, therefore, serve as a novel cancer target
Title: Neddylation E2 UBE2F Promotes the Survival of Lung Cancer Cells by Activating CRL5 to Degrade NOXA via the K11 Linkage.
Distinct preferences of UBC12 and UBE2F peptides for inhibiting different DCNLs, including the oncogenic DCNL1.
Title: Structural conservation of distinctive N-terminal acetylation-dependent interactions across a family of mammalian NEDD8 ligation enzymes.

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Phenotypes

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Find tests for this gene in the NIH Genetic Testing Registry (GTR)

Review eQTL and phenotype association data in this region using PheGenI

Variation

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See variants in ClinVar

See studies and variants in dbVar

See Variation Viewer (GRCh37.p13)

See Variation Viewer (GRCh38)

HIV-1 interactions

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Protein interactions

Protein	Gene	Interaction	Pubs
Vif	vif	The conjugation of NEDD8 to Cullin-5 by UBE2F is required for HIV-1 Vif-mediated A3G degradation	PubMed
	vif	The active site proximal loop extension (residues 124-129) in UBE2F is critical for HIV-1 Vif-mediated A3G degradation	PubMed
	vif	UBE2F and RBX2 are required for activation of the polyubiquitin synthesis activity of Vif/CBF-beta/CUL5, leading to HIV-1 Vif-mediated degradation of A3G in cells	PubMed

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

G35018 (e-PCR)
- UniSTS:70683

RH68499 (e-PCR)
- UniSTS:55675

Readthrough UBE2F-SCLY

Readthrough gene: UBE2F-SCLY, Included gene: SCLY

Gene Ontology Provided by GOA

Function	Evidence Code	Pubs
enables ATP binding	IEA Inferred from Electronic Annotation more info
enables NEDD8 conjugating enzyme activity	IDA Inferred from Direct Assay more info	PubMed
enables NEDD8 ligase activity	IEA Inferred from Electronic Annotation more info
enables NEDD8 transferase activity	IBA Inferred from Biological aspect of Ancestor more info
enables NEDD8 transferase activity	TAS Traceable Author Statement more info
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed

Process	Evidence Code	Pubs
involved_in post-translational protein modification	TAS Traceable Author Statement more info
involved_in protein neddylation	IBA Inferred from Biological aspect of Ancestor more info
involved_in protein neddylation	IDA Inferred from Direct Assay more info	PubMed
involved_in protein neddylation	IEA Inferred from Electronic Annotation more info

Component	Evidence Code	Pubs
located_in cytosol	TAS Traceable Author Statement more info
is_active_in nucleus	IBA Inferred from Biological aspect of Ancestor more info

General protein information

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Preferred Names: NEDD8-conjugating enzyme UBE2F

Names: NEDD8 carrier protein UBE2F; NEDD8 protein ligase UBE2F; NEDD8-conjugating enzyme 2; RING-type E3 NEDD8 transferase UBE2F; ubiquitin-conjugating enzyme E2F (putative)

NP_001265234.1

EC 2.3.2.32

NP_001265235.1

EC 2.3.2.32

NP_001265236.1

EC 2.3.2.32

NP_001265237.1

EC 2.3.2.32

NP_542409.1

EC 2.3.2.32

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_001278305.2 → NP_001265234.1 NEDD8-conjugating enzyme UBE2F isoform 1

See identical proteins and their annotated locations for NP_001265234.1

Status: VALIDATED

Description

Transcript Variant: This variant (2) differs in the 5' UTR, compared to variant 1. Both variants 1 and 2 encode the same isoform (1).

Source sequence(s)

AC016776, AK294107, CN370973, CN370974, DA140546, DB118122

Consensus CDS

CCDS2523.1

UniProtKB/Swiss-Prot

A8K1Z8, B4DDT9, B4DFI1, B4DMK3, B4DZU2, B8ZZG2, C9J212, H9KVB9, Q969M7

UniProtKB/TrEMBL

C9J9P8

Related

ENSP00000478474.1, ENST00000612130.4

Conserved Domains (1) summary

cl00154
Location:30 → 184

UBCc; Ubiquitin-conjugating enzyme E2, catalytic (UBCc) domain. This is part of the ubiquitin-mediated protein degradation pathway in which a thiol-ester linkage forms between a conserved cysteine and the C-terminus of ubiquitin and complexes with ubiquitin ...
NM_001278306.2 → NP_001265235.1 NEDD8-conjugating enzyme UBE2F isoform 2

See identical proteins and their annotated locations for NP_001265235.1

Status: VALIDATED

Description

Transcript Variant: This variant (3) differs in the 5' UTR, lacks a portion of the 5' coding region, and initiates translation at an alternate upstream start codon, compared to variant 1. The encoded isoform (2) is shorter than isoform 1 and has a distinct N-terminus.

Source sequence(s)

AK294107, BM470609, BX421077, CN370973, CN370974

Consensus CDS

CCDS63175.1

UniProtKB/Swiss-Prot

Q969M7

Related

ENSP00000386680.1, ENST00000409953.5

Conserved Domains (1) summary

COG5078
Location:16 → 160

COG5078; Ubiquitin-protein ligase [Posttranslational modification, protein turnover, chaperones]
NM_001278307.2 → NP_001265236.1 NEDD8-conjugating enzyme UBE2F isoform 3

See identical proteins and their annotated locations for NP_001265236.1

Status: VALIDATED

Description

Transcript Variant: This variant (4) lacks two alternate exons in the 5' coding region, but maintains the reading frame, compared to variant 1. The encoded isoform (3) is shorter than isoform 1.

Source sequence(s)

AK297502, CN370973, CN370974

Consensus CDS

CCDS63177.1

UniProtKB/TrEMBL

C9JF62

Related

ENSP00000399183.1, ENST00000414443.5

Conserved Domains (1) summary

cl00154
Location:40 → 152

UBCc; Ubiquitin-conjugating enzyme E2, catalytic (UBCc) domain. This is part of the ubiquitin-mediated protein degradation pathway in which a thiol-ester linkage forms between a conserved cysteine and the C-terminus of ubiquitin and complexes with ubiquitin ...
NM_001278308.2 → NP_001265237.1 NEDD8-conjugating enzyme UBE2F isoform 4

See identical proteins and their annotated locations for NP_001265237.1

Status: VALIDATED

Description

Transcript Variant: This variant (5) lacks an alternate exon in the 3' coding region, but maintains the reading frame, compared to variant 1. The encoded isoform (4) is shorter than isoform 1.

Source sequence(s)

AK294107, BQ959565, BX421077, CN370973, CN370974

Consensus CDS

CCDS63176.1

UniProtKB/TrEMBL

C9J9P8

Related

ENSP00000387299.1, ENST00000409633.5

Conserved Domains (1) summary

cd00195
Location:35 → 149

UBCc; Ubiquitin-conjugating enzyme E2, catalytic (UBCc) domain. This is part of the ubiquitin-mediated protein degradation pathway in which a thiol-ester linkage forms between a conserved cysteine and the C-terminus of ubiquitin and complexes with ubiquitin ...
NM_080678.3 → NP_542409.1 NEDD8-conjugating enzyme UBE2F isoform 1

See identical proteins and their annotated locations for NP_542409.1

Status: VALIDATED

Description

Transcript Variant: This variant (1) encodes the longest isoform (1). Both variants 1 and 2 encode the same isoform (1).

Source sequence(s)

AK290063, AK294107, BC010549, BX421077, CN370973

Consensus CDS

CCDS2523.1

UniProtKB/Swiss-Prot

A8K1Z8, B4DDT9, B4DFI1, B4DMK3, B4DZU2, B8ZZG2, C9J212, H9KVB9, Q969M7

UniProtKB/TrEMBL

C9J9P8

Related

ENSP00000272930.4, ENST00000272930.9

Conserved Domains (1) summary

cl00154
Location:30 → 184

UBCc; Ubiquitin-conjugating enzyme E2, catalytic (UBCc) domain. This is part of the ubiquitin-mediated protein degradation pathway in which a thiol-ester linkage forms between a conserved cysteine and the C-terminus of ubiquitin and complexes with ubiquitin ...

RNA

NR_103498.2 RNA Sequence

Status: VALIDATED

Description

Transcript Variant: This variant (6) lacks an alternate internal exon, compared to variant 1. This variant is represented as non-coding because the use of the 5'-most expected translational start codon, as used in variant 1, renders the transcript a candidate for nonsense-mediated mRNA decay (NMD).

Source sequence(s)

AK294107, BX421077, CN370973, CN370974
NR_103499.2 RNA Sequence

Status: VALIDATED

Description

Transcript Variant: This variant (7) lacks two alternate internal exons, compared to variant 1. This variant is represented as non-coding because the use of the 5'-most expected translational start codon, as used in variant 1, renders the transcript a candidate for nonsense-mediated mRNA decay (NMD).

Source sequence(s)

AK303094, BQ959565, BX421077, CN370973, CN370974
NR_103500.2 RNA Sequence

Status: VALIDATED

Description

Transcript Variant: This variant (8) contains an alternate internal exon, compared to variant 1. This variant is represented as non-coding because the use of the 5'-most expected translational start codon, as used in variant 1, renders the transcript a candidate for nonsense-mediated mRNA decay (NMD).

Source sequence(s)

AK294107, BM920749, BX421077, CN370973, CN370974