NT5E 5'-nucleotidase ecto [Homo sapiens (human)] - Gene

Summary

Official Symbol: NT5Eprovided by HGNC
Official Full Name: 5'-nucleotidase ectoprovided by HGNC
Primary source: HGNC:HGNC:8021
See related: Ensembl:ENSG00000135318 MIM:129190; AllianceGenome:HGNC:8021
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Homo sapiens
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo
Also known as: NT; eN; NT5; NTE; eNT; CD73; E5NT; CALJA
Summary: The protein encoded by this gene is a plasma membrane protein that catalyzes the conversion of extracellular nucleotides to membrane-permeable nucleosides. The encoded protein is used as a determinant of lymphocyte differentiation. Defects in this gene can lead to the calcification of joints and arteries. Two transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Mar 2011]
Expression: Ubiquitous expression in endometrium (RPKM 30.0), ovary (RPKM 19.6) and 22 other tissues See more
Orthologs: mouse all
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Genomic context

Location:: 6q14.3

Exon count:: 9

Annotation release	Status	Assembly	Chr	Location
RS_2023_10	current	GRCh38.p14 (GCF_000001405.40)	6	NC_000006.12 (85450083..85495784)
RS_2023_10	current	T2T-CHM13v2.0 (GCF_009914755.1)	6	NC_060930.1 (86666882..86712587)
105.20220307	previous assembly	GRCh37.p13 (GCF_000001405.25)	6	NC_000006.11 (86159801..86205502)

Chromosome 6 - NC_000006.12 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: HPA RNA-seq normal tissues
Description: RNA-seq was performed of tissue samples from 95 human individuals representing 27 different tissues in order to determine tissue-specificity of all protein-coding genes
BioProject: PRJEB4337
Publication: PMID 24309898
Analysis date: Wed Apr 4 07:08:55 2018

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

CD73 as a T cell dysfunction marker predicting cardiovascular and infection events in patients undergoing hemodialysis.
Title: CD73 as a T cell dysfunction marker predicting cardiovascular and infection events in patients undergoing hemodialysis.
Altered CD39 and CD73 Expression in Rheumatoid Arthritis: Implications for Disease Activity and Treatment Response.
Title: Altered CD39 and CD73 Expression in Rheumatoid Arthritis: Implications for Disease Activity and Treatment Response.
Human plasmacytoid dendritic cells express the functional purinergic halo (CD39/CD73).
Title: Human plasmacytoid dendritic cells express the functional purinergic halo (CD39/CD73).
CD73 contributes to the pathogenesis of fusion-negative rhabdomyosarcoma through the purinergic signaling pathway.
Title: CD73 contributes to the pathogenesis of fusion-negative rhabdomyosarcoma through the purinergic signaling pathway.
The hypoxia-regulated ectonucleotidase CD73 is a host determinant of HIV latency.
Title: The hypoxia-regulated ectonucleotidase CD73 is a host determinant of HIV latency.
Effects of abnormal expression of CD73 on malignant phenotype of nasopharyngeal carcinoma.
Title: Effects of abnormal expression of CD73 on malignant phenotype of nasopharyngeal carcinoma.
CD73 controls Myosin II-driven invasion, metastasis, and immunosuppression in amoeboid pancreatic cancer cells.
Title: CD73 controls Myosin II-driven invasion, metastasis, and immunosuppression in amoeboid pancreatic cancer cells.
Integrative analyses identify CD73 as a prognostic biomarker and immunotherapeutic target in intrahepatic cholangiocarcinoma.
Title: Integrative analyses identify CD73 as a prognostic biomarker and immunotherapeutic target in intrahepatic cholangiocarcinoma.
5'-Ectonucleotidase CD73/NT5E supports EGFR-mediated invasion of HPV-negative head and neck carcinoma cells.
Title: 5'-Ectonucleotidase CD73/NT5E supports EGFR-mediated invasion of HPV-negative head and neck carcinoma cells.
The CD73 is induced by TGF-beta1 triggered by nutrient deprivation and highly expressed in dedifferentiated human melanoma.
Title: The CD73 is induced by TGF-β1 triggered by nutrient deprivation and highly expressed in dedifferentiated human melanoma.

Submit: New GeneRIF Correction See all GeneRIFs (229)

Phenotypes

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Find tests for this gene in the NIH Genetic Testing Registry (GTR)

Review eQTL and phenotype association data in this region using PheGenI

Associated conditions

Description	Tests
Hereditary arterial and articular multiple calcification syndrome MedGen: C1859372 OMIM: 211800 GeneReviews: Not available	Compare labs

EBI GWAS Catalog

Description
An atlas of genetic influences on human blood metabolites. EBI GWAS Catalog EBI GWAS CatalogPubMed
Human metabolic individuality in biomedical and pharmaceutical research. EBI GWAS Catalog EBI GWAS CatalogPubMed

Variation

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See variants in ClinVar

See studies and variants in dbVar

See Variation Viewer (GRCh37.p13)

See Variation Viewer (GRCh38)

HIV-1 interactions

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Protein interactions

Protein	Gene	Interaction	Pubs
Vif	vif	HIV-1 Vif upregulates the expression of 5'-nucleotidase, ecto (NT5E, CD73) in Vif-expression T cells	PubMed

Go to the HIV-1, Human Interaction Database

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

RH99003 (e-PCR)
- UniSTS:88425

RH46710 (e-PCR)
- UniSTS:21108

SHGC-81763 (e-PCR)
- UniSTS:184472

SHGC-80786 (e-PCR)
- UniSTS:102310

STS-X55740 (e-PCR)
- UniSTS:73678

WI-20546 (e-PCR)
- UniSTS:46296

D6S1847 (e-PCR)
- UniSTS:38875

Gene Ontology Provided by GOA

Function	Evidence Code	Pubs
enables 5'-deoxynucleotidase activity	IDA Inferred from Direct Assay more info	PubMed
enables 5'-nucleotidase activity	IBA Inferred from Biological aspect of Ancestor more info
enables 5'-nucleotidase activity	IDA Inferred from Direct Assay more info	PubMed
enables 5'-nucleotidase activity	IMP Inferred from Mutant Phenotype more info	PubMed
enables GMP 5'-nucleotidase activity	IDA Inferred from Direct Assay more info	PubMed
enables IMP 5'-nucleotidase activity	IDA Inferred from Direct Assay more info	PubMed
enables XMP 5'-nucleosidase activity	IEA Inferred from Electronic Annotation more info
enables identical protein binding	IDA Inferred from Direct Assay more info	PubMed
enables nucleotide binding	IEA Inferred from Electronic Annotation more info
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables thymidylate 5'-phosphatase activity	IDA Inferred from Direct Assay more info	PubMed
enables zinc ion binding	IDA Inferred from Direct Assay more info	PubMed

Process	Evidence Code	Pubs
involved_in ADP catabolic process	IEA Inferred from Electronic Annotation more info
involved_in AMP catabolic process	IBA Inferred from Biological aspect of Ancestor more info
involved_in ATP metabolic process	IDA Inferred from Direct Assay more info	PubMed
involved_in DNA metabolic process	TAS Traceable Author Statement more info	PubMed
involved_in adenosine biosynthetic process	IEA Inferred from Electronic Annotation more info
involved_in calcium ion homeostasis	IEA Inferred from Electronic Annotation more info
involved_in inhibition of non-skeletal tissue mineralization	IEA Inferred from Electronic Annotation more info
involved_in leukocyte cell-cell adhesion	IDA Inferred from Direct Assay more info	PubMed
involved_in negative regulation of inflammatory response	IEA Inferred from Electronic Annotation more info
involved_in response to ATP	IDA Inferred from Direct Assay more info	PubMed
involved_in response to inorganic substance	IDA Inferred from Direct Assay more info	PubMed

Component	Evidence Code	Pubs
located_in cell surface	HDA more info	PubMed
located_in cytosol	IDA Inferred from Direct Assay more info
located_in external side of plasma membrane	IEA Inferred from Electronic Annotation more info
located_in extracellular exosome	HDA more info	PubMed
located_in membrane	IDA Inferred from Direct Assay more info	PubMed
located_in nucleoplasm	IDA Inferred from Direct Assay more info
is_active_in plasma membrane	IBA Inferred from Biological aspect of Ancestor more info
is_active_in plasma membrane	IDA Inferred from Direct Assay more info	PubMed
located_in plasma membrane	IDA Inferred from Direct Assay more info
located_in plasma membrane	TAS Traceable Author Statement more info

General protein information

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Preferred Names: 5'-nucleotidase

Names: 5'-NT; 5'-deoxynucleotidase; IMP-specific 5'-nucleotidase; Purine 5-Prime-Nucleotidase; ecto-5'-nucleotidase; thymidylate 5'-phosphatase

NP_001191742.1

EC 3.1.3.35

EC 3.1.3.5

EC 3.1.3.89

EC 3.1.3.91

EC 3.1.3.99

NP_002517.1

EC 3.1.3.35

EC 3.1.3.5

EC 3.1.3.89

EC 3.1.3.91

EC 3.1.3.99

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

Genomic

NG_028214.1 RefSeqGene

Range

5500..51201

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001204813.2 → NP_001191742.1 5'-nucleotidase isoform 2 preproprotein

See identical proteins and their annotated locations for NP_001191742.1

Status: REVIEWED

Description

Transcript Variant: This variant (2) lacks an alternate in-frame exon compared to variant 1. The resulting isoform (2) has the same N- and C-termini but is shorter compared to isoform 1.

Source sequence(s)

AK075008, BC065937, BQ019358, DA574510, X55740

Consensus CDS

CCDS56439.1

UniProtKB/TrEMBL

B2RBH2

Related

ENSP00000358665.3, ENST00000369651.7

Conserved Domains (3) summary

COG0737
Location:11 → 482

UshA; 2',3'-cyclic-nucleotide 2'-phosphodiesterase/5'- or 3'-nucleotidase, 5'-nucleotidase family [Nucleotide transport and metabolism, Defense mechanisms]

cd07409
Location:29 → 310

MPP_CD73_N; CD73 ecto-5'-nucleotidase and related proteins, N-terminal metallophosphatase domain

pfam02872
Location:339 → 457

5_nucleotid_C; 5'-nucleotidase, C-terminal domain
NM_002526.4 → NP_002517.1 5'-nucleotidase isoform 1 preproprotein

See identical proteins and their annotated locations for NP_002517.1

Status: REVIEWED

Description

Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1).

Source sequence(s)

AK075008, BC065937, BQ019358, DA574510, X55740

Consensus CDS

CCDS5002.1

UniProtKB/Swiss-Prot

B3KQI8, O75520, P21589, Q5W116

UniProtKB/TrEMBL

B2RBH2

Related

ENSP00000257770.3, ENST00000257770.8

Conserved Domains (3) summary

COG0737
Location:11 → 532

UshA; 2',3'-cyclic-nucleotide 2'-phosphodiesterase/5'- or 3'-nucleotidase, 5'-nucleotidase family [Nucleotide transport and metabolism, Defense mechanisms]

cd07409
Location:29 → 310

MPP_CD73_N; CD73 ecto-5'-nucleotidase and related proteins, N-terminal metallophosphatase domain

pfam02872
Location:339 → 507

5_nucleotid_C; 5'-nucleotidase, C-terminal domain