| Submitter Method Handle: | DDI | | Submitter Method ID: | WGS | | Submitted method description: | |
| | Prior to library preparation, frozen DNA stock was diluted to a working solution of 50 ng/µl as recommended by Illumina (CA, USA) and was quantified by agarose gel analysis. DNA sample was sheared | | | to generate uniform size segments averaging around 400 bps by using Covaris E220 instrument (Covaris, Woburn, MA, USA) with the parameters of Duty Cycle 10%; Intensity 4; Cycles per Burst 200; and | | | Time 55 seconds. Sheared DNA was used to prepare sequencing libraries using TruSeq DNA sample preparation and cBot Paired End (PE) cluster generation kits as per manufacturer?s protocols (Illumina, | | | CA, USA). Libraries were loaded on a HiSeq 2000 flow cell for paired-end sequencing using the TruSeq SBS 200 cycles chemistry. |
This method was used in the following submission:
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