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Method Detail
Submitter Method Handle: DDI
Submitter Method ID: WGS
Submitted method description:
Prior to library preparation, frozen DNA stock was diluted to a working solution of 50 ng/Ál as recommended by Illumina (CA, USA) and was quantified by agarose gel analysis. DNA sample was sheared
to generate uniform size segments averaging around 400 bps by using Covaris E220 instrument (Covaris, Woburn, MA, USA) with the parameters of Duty Cycle 10%; Intensity 4; Cycles per Burst 200; and
Time 55 seconds. Sheared DNA was used to prepare sequencing libraries using TruSeq DNA sample preparation and cBot Paired End (PE) cluster generation kits as per manufacturer?s protocols (Illumina,
CA, USA). Libraries were loaded on a HiSeq 2000 flow cell for paired-end sequencing using the TruSeq SBS 200 cycles chemistry.

This method was used in the following submission:

Submitter Handle Batch Type Submitter batch id Release build id
DDI Genotype WGS - SNPs 146
DDI Genotype WGS - INDELS 146
DDI Assay WGS - SNPs 142
DDI Assay WGS - indels 142