GSM3442856: Wild type intestinal spheroid cells; Mus musculus; ChIP-S... - SRA

SRX4921592: GSM3442856: Wild type intestinal spheroid cells; Mus musculus; ChIP-Seq
6 ILLUMINA (Illumina HiSeq 2500) runs: 22.2M spots, 4.5G bases, 1.5Gb downloads

Submitted by: NCBI (GEO)

Study: Essential role of Arid1a in intestinal stem cell maintenance and homeostasis through Sox9 regulation (ChIP-Seq)

PRJNA498053 • SRP166448 • All experiments • All runs

show Abstracthide Abstract

Arid1a, a subunit of the SWI/SNF chromatin remodeling complex, have been reported in multiple human cancers, however, its functional role in intestinal homeostasis remains unclear. To investigate the role of Arid1a in murine intestine, we have employed ChIP experiments in intestinal spheroid cells generated from crypt cells of wild type mice. ChIP assay revealed that Arid1a binds directly to the Sox9 promoter to support its expression. Overall design: We used intestinal spheroid cells generated from crypt cells of wild type mice.

Sample: Wild type intestinal spheroid cells

SAMN10279369 • SRS3966919 • All experiments • All runs

Organism: Mus musculus

Library:

Instrument: Illumina HiSeq 2500

Strategy: ChIP-Seq

Source: GENOMIC

Selection: ChIP

Layout: PAIRED

Construction protocol: Lysates were clarified from sonicated nuclei and histone-DNA complexes were isolated with antibody. Sequencing libraries were prepared using the TruSeq ChIP Sample Prep Kit (Illumina) following the manufacturer's instructions. The libraries were sequenced on the HiSeq 2500 sequencing platform using HiSeq SBS Kit v4 reagents with 2 x 101 cycles.

Experiment attributes:

GEO Accession: GSM3442856

Links:

NCBI link: NCBI Entrez (gds)

Runs: 6 runs, 22.2M spots, 4.5G bases, 1.5Gb

Run	# of Spots	# of Bases	Size	Published
SRR8094735	4,000,000	808M	278.3Mb	2018-10-25
SRR8094736	4,000,000	808M	290.5Mb	2018-10-25
SRR8094738	4,000,000	808M	280.5Mb	2018-10-25
SRR8094739	4,000,000	808M	263.8Mb	2018-10-25
SRR8094740	4,000,000	808M	267.4Mb	2018-10-25
SRR8094741	2,162,313	436.8M	149.7Mb	2018-10-25

ID:: 6622952

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