|
Status |
Public on May 03, 2016 |
Title |
Adults B-cell ALL Peripheral blood, sample 136 |
Sample type |
genomic |
|
|
Channel 1 |
Source name |
Peripheral blood cells (test)
|
Organism |
Homo sapiens |
Characteristics |
cell type: Peripheral blood cells gender: Female disease state: B-cell acute lymphoblastic leukemia age group: Adults ≥18 years genetic subtype: BCR-ABL1 treatment: LAL-070PH
|
Extracted molecule |
genomic DNA |
Extraction protocol |
The genomic DNA was extracted from frozen bone marrow or fixed peripheral blood cell samples with QIAmp DNA Mini Kit (Qiagen, 16 Valencia, CA, USA) following the manufacturer’s instructions
|
Label |
Cy3
|
Label protocol |
Samples were denatured and labeled in parallel with Cy3 for the test group and Cy5 for the control group, each through a random priming method, using Klenow fragments (NimbleGen Dual-Color DNA Labeling Kit, Roche NimbleGen, Inc., Madison, WI, USA)
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|
|
Channel 2 |
Source name |
control
|
Organism |
Homo sapiens |
Characteristics |
gender: Female disease state: Not applicable age group: Not applicable treatment: Not applicable reference composition: Human Genomic DNA: Female, Promega, Madison, WI, USA
|
Extracted molecule |
genomic DNA |
Extraction protocol |
The genomic DNA was extracted from frozen bone marrow or fixed peripheral blood cell samples with QIAmp DNA Mini Kit (Qiagen, 16 Valencia, CA, USA) following the manufacturer’s instructions
|
Label |
Cy5
|
Label protocol |
Samples were denatured and labeled in parallel with Cy3 for the test group and Cy5 for the control group, each through a random priming method, using Klenow fragments (NimbleGen Dual-Color DNA Labeling Kit, Roche NimbleGen, Inc., Madison, WI, USA)
|
|
|
|
Hybridization protocol |
Hybridization was performed according to the instructions in the NimbleGen Array User Guide
|
Scan protocol |
Scanning was performed according to the instructions in the NimbleGen Array User Guide
|
Data processing |
Arrays were processed using Nimblegen's standard protocol and segmented using the CGHweb tool.
|
|
|
Submission date |
Dec 03, 2015 |
Last update date |
May 03, 2016 |
Contact name |
Jesús Maria Hernández-Rivas |
E-mail(s) |
jmhr@usal.es
|
Organization name |
Hospital Universitario de Salamanca, Universidad de Salamanca-CSIC
|
Department |
Hematology
|
Street address |
Paseo San Vicente, 88-182
|
City |
Salamanca |
State/province |
Salamanca |
ZIP/Postal code |
37007 |
Country |
Spain |
|
|
Platform ID |
GPL16707 |
Series (1) |
GSE75671 |
Genome-Wide DNA Copy Number Analysis of Acute Lymphoblastic Leukemia Identifies New Genetic Markers Associated with Clinical Outcome |
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